{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,13]],"date-time":"2026-09-13T05:02:06Z","timestamp":1789275726420,"version":"build-2803163510"},"reference-count":50,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2025,9,30]],"date-time":"2025-09-30T00:00:00Z","timestamp":1759190400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,9,30]],"date-time":"2025-09-30T00:00:00Z","timestamp":1759190400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Aging Cell"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Genomic instability is a hallmark of aging and cancer. A key contributor to genomic instability includes alternative DNA structures, such as cruciform\u2010forming inverted repeats (IRs). Short IRs (&lt;\u2009100\u2009bps) are abundant in the human genome, mutagenic, and enriched at mutation hotspots in human cancer genomes. Using an innovative mutation\u2010reporter mouse model, we showed that short IRs are mutagenic in\u00a0vivo. Further, we found that aging exacerbates IR\u2010induced genomic instability, as evidenced by increased mutation frequencies and altered spectra in the spleen and brain of mice harboring either a short IR or control B\u2010DNA sequence at 2 and 24\u2009months of age. These findings establish a link between aging and enhanced mutagenesis at short IRs, providing a unique in\u00a0vivo platform to investigate age\u2010related mechanisms of DNA structure\u2010mediated genomic instability.<\/jats:p>","DOI":"10.1111\/acel.70250","type":"journal-article","created":{"date-parts":[[2025,9,30]],"date-time":"2025-09-30T10:26:39Z","timestamp":1759227999000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Aging Increases Short, Inverted Repeat\u2010Mediated Genomic Instability In\u00a0Vivo"],"prefix":"10.1111","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5410-6968","authenticated-orcid":false,"given":"Pooja","family":"Mandke","sequence":"first","affiliation":[{"name":"Dell Pediatric Research Institute, Division of Pharmacology and Toxicology College of Pharmacy, the University of Texas at Austin  Austin Texas 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