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We engineered an ovarian somatic organoid model by culturing a stroma\u2010enriched fraction of mouse ovaries in scaffold\u2010free agarose micromolds. Self\u2010organized ovarian somatic organoids maintained diverse cell populations, produced extracellular matrix, and secreted hormones. Organoids generated from reproductively old mice exhibited reduced aggregation and growth compared to young counterparts, as well as differences in cellular composition. Interestingly, matrix fibroblasts from old mice demonstrated upregulation of pathways associated with the actin cytoskeleton and downregulation of cell adhesion pathways, indicative of increased cellular stiffness that may impair organoid aggregation. Cellular morphology, which is regulated by the cytoskeleton, significantly changed with age and in response to actin modulation. Moreover, actin modulation altered organoid aggregation efficiency. Overall, ovarian somatic organoids have advanced knowledge of cellular contributions to ovarian aging.<\/jats:p>","DOI":"10.1111\/acel.70333","type":"journal-article","created":{"date-parts":[[2025,12,29]],"date-time":"2025-12-29T13:15:33Z","timestamp":1767014133000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Self\u2010Organizing Ovarian Somatic Organoids Preserve Cellular Heterogeneity and Reveal Cellular Contributions to Ovarian Aging"],"prefix":"10.1111","volume":"25","author":[{"given":"Shweta S.","family":"Dipali","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynecology, Feinberg School of Medicine Northwestern University  Chicago Illinois USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4203-1687","authenticated-orcid":false,"given":"Aubrey","family":"Converse","sequence":"additional","affiliation":[{"name":"Department of 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