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We examined the cellular composition and transcriptional profiles using single\u2010nuclei RNA sequencing in IMAT from 6 older women with KOA and knee pain and 5 older women without KOA or knee pain from the Study of Muscle, Mobility and Aging (SOMMA). From the resulting 21,436 nuclei, we identified 6 major cell types with unique transcriptional profiles, including progenitor cells, adipocytes, macrophages and other immune cells (T\/B\/NK cells), endothelial cells and smooth muscle cells\/pericytes. Sub\u2010clustering of the immune cell population revealed the presence of mast cells and B\u2010cells with greater abundances in the KOA group. The adipocyte population was the most transcriptional diverse population between the KOA group and the group without KOA. 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