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However, tissue\u2010specific mechanisms of aging, specifically the contributions of the urothelium, remain elusive. Here, we introduce mouse bladder epithelium\u2010derived organoids (mBEDOs) as a scalable platform to model urothelial aging. mBEDOs from aged mice recapitulate key features of age\u2010associated cellular reprogramming, including oxidative stress, senescence, and DNA damage. We demonstrate the utility of mBEDOs for modeling Uropathogenic\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Escherichia coli<\/jats:italic>\n                    <\/jats:styled-content>\n                    (UPEC) infection, generating assembloids between mBEDOs and macrophages to model epithelial\u2010immune interactions, and genetic perturbation. Using the mBEDO platform, we also identify urothelium\u2010specific changes in purine, amino acid, and glycerophospholipid metabolism, which may contribute to age\u2010associated cellular perturbations. Lastly, supplementation with depleted metabolites, nicotinamide and\n                    <jats:sc>d<\/jats:sc>\n                    \u2010mannose, reduces DNA damage and oxidative stress and restores mitochondrial integrity in aged mBEDOs. These findings establish mBEDOs as an effective platform for investigating molecular and cellular underpinnings of urothelial aging and exploring metabolism\u2010based interventions for age\u2010associated bladder dysfunction.\n                  <\/jats:p>","DOI":"10.1111\/acel.70391","type":"journal-article","created":{"date-parts":[[2026,1,24]],"date-time":"2026-01-24T02:36:21Z","timestamp":1769222181000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["A Scalable Organoid Model of Urothelial Aging for Metabolic Interrogation, Infection Modeling, and Reversal of Age\u2010Associated Changes"],"prefix":"10.1111","volume":"25","author":[{"given":"Adwaita R.","family":"Parab","sequence":"first","affiliation":[{"name":"Department of Medicine, Section of Infectious Diseases Baylor College of Medicine  Houston Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9795-5799","authenticated-orcid":false,"given":"Arnold M.","family":"Salazar","sequence":"additional","affiliation":[{"name":"Department of Medicine, Section of Infectious Diseases Baylor College of Medicine  Houston Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven J.","family":"Bark","sequence":"additional","affiliation":[{"name":"Department of Medicine, Section of Infectious Diseases Baylor College of Medicine  Houston Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Margarita","family":"Divenko","sequence":"additional","affiliation":[{"name":"Department of Medicine, Section of Gastroenterology Baylor College of Medicine  Houston Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vasanta","family":"Putluri","sequence":"additional","affiliation":[{"name":"Advanced Technology Cores Baylor College of Medicine  Houston Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D'Feau J.","family":"Lieu","sequence":"additional","affiliation":[{"name":"Department of Medicine, Section of Infectious Diseases Baylor College of Medicine  Houston Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aadya S.","family":"Singh","sequence":"additional","affiliation":[{"name":"Department of Medicine, Section of Infectious Diseases Baylor College of Medicine  Houston Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nagireddy","family":"Putluri","sequence":"additional","affiliation":[{"name":"Advanced Technology Cores Baylor College of Medicine  Houston Texas USA"},{"name":"Department of Molecular and Cellular Biology Baylor College of Medicine  Houston Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3917-8677","authenticated-orcid":false,"given":"Indira 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