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However, the underlying mechanisms remain unclear. Here, we investigated the role of the myosin co\u2010chaperone, uncoordinated mutant number\u201045 myosin chaperone B (UNC45B), in regulating muscle mass and force. UNC45B expression decreased in mouse gastrocnemius muscle with age, particularly at 24\u2009months old, and adeno\u2010associated virus vector\u2010mediated knockdown of\n                    <jats:italic>Unc45b<\/jats:italic>\n                    in 3\u2010month\u2010old mouse triceps surae muscle first reduced plantar flexor torque and then decreased gastrocnemius muscle mass. In addition,\n                    <jats:italic>Unc45b<\/jats:italic>\n                    knockdown in the triceps surae muscle resulted in lower bone mineral density. While maximum Ca\n                    <jats:sup>2+<\/jats:sup>\n                    \u2010activated force in mechanically skinned fibers was not affected by\n                    <jats:italic>Unc45b<\/jats:italic>\n                    knockdown,\n                    <jats:italic>Unc45b<\/jats:italic>\n                    knockdown decreased the ratio of depolarization\u2010induced force to the maximum Ca\n                    <jats:sup>2+<\/jats:sup>\n                    \u2010activated force. We established tamoxifen\u2010inducible skeletal muscle\u2010specific\n                    <jats:italic>Unc45b<\/jats:italic>\n                    knockout (\n                    <jats:italic>Unc45b<\/jats:italic>\n                    imKO) mice to investigate whether the muscle atrophy and weakness due to the loss of\n                    <jats:italic>Unc45b<\/jats:italic>\n                    impacts metabolism and behavior. We found that\n                    <jats:italic>Unc45b<\/jats:italic>\n                    imKO reduced muscle mass and force at a whole\u2010body level, but did not influence systemic glucose tolerance, insulin sensitivity, or the respiratory exchange ratio. However,\n                    <jats:italic>Unc45b<\/jats:italic>\n                    imKO mice reduced the amount of deeper non\u2010rapid eye movement sleep, locomotor activity, and body temperature during the sleep phase. We conclude that UNC45B is essential for maintaining fast\u2010twitch muscle mass and muscle force. In addition,\n                    <jats:italic>Unc45b<\/jats:italic>\n                    deficiency\u2010mediated muscle loss is also associated with bone fragility, decreased body temperature, and impaired sleep quality.\n                  <\/jats:p>","DOI":"10.1111\/acel.70502","type":"journal-article","created":{"date-parts":[[2026,4,21]],"date-time":"2026-04-21T05:37:38Z","timestamp":1776749858000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["<scp>UNC45B<\/scp>\n                    Reduction With Aging: A Myofiber\u2010Intrinsic Promoting Factor for Sarcopenia"],"prefix":"10.1111","volume":"25","author":[{"given":"Taiga","family":"Mishima","sequence":"first","affiliation":[{"name":"Cellular and Molecular Biotechnology Research Institute National Institute of Advanced Industrial Science and Technology (AIST)  Tsukuba Japan"},{"name":"Department of Life Science and Applied Chemistry Nagoya Institute of 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