{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T17:06:55Z","timestamp":1789924015174,"version":"4.0.1"},"reference-count":74,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,5,3]],"date-time":"2026-05-03T00:00:00Z","timestamp":1777766400000},"content-version":"vor","delay-in-days":2,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T00:00:00Z","timestamp":1777593600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Aging Cell"],"published-print":{"date-parts":[[2026,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Vitamin K2 is a fat\u2010soluble vitamin that has been reported to exhibit significant anti\u2010stress activity. Anti\u2010stress properties are considered to be closely associated with lifespan extension. Therefore, we investigated the effects of vitamin K2 on the lifespan and stress resistance of\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Caenorhabditis elegans<\/jats:italic>\n                    <\/jats:styled-content>\n                    , as well as the underlying mechanisms. In the present study, we found that the effects of Vitamin K2 on\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>C.\u2009elegans<\/jats:italic>\n                    <\/jats:styled-content>\n                    are concentration\u2010dependent. High concentrations (10\u2009\u03bcM) of Vitamin K2 are toxic to\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>C.\u2009elegans<\/jats:italic>\n                    <\/jats:styled-content>\n                    , whereas lower concentrations (5\u2009\u03bcM) are beneficial. Treatment with 5\u2009\u03bcM Vitamin K2 can extend the lifespan of\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>C.\u2009elegans<\/jats:italic>\n                    <\/jats:styled-content>\n                    , enhance its physiological functions, protect the intestinal barrier, and reduce the accumulation of lipofuscin associated with aging. Furthermore, Vitamin K2 enhanced the stress resistance of\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>C.\u2009elegans<\/jats:italic>\n                    <\/jats:styled-content>\n                    by maintaining mitochondrial morphology, alleviating mitochondrial stress, reducing ROS levels, and improving mitochondrial membrane potential and ATP production. Vitamin K2 activates the JNK\u20101\/SIR\u20102.1\/DAF\u201016 signaling pathway and upregulates the expression of downstream target genes such as\n                    <jats:italic>ctl\u20101<\/jats:italic>\n                    ,\n                    <jats:italic>ctl\u20102<\/jats:italic>\n                    ,\n                    <jats:italic>sod\u20101<\/jats:italic>\n                    ,\n                    <jats:italic>sod\u20103<\/jats:italic>\n                    , and\n                    <jats:italic>hsp\u201016.2<\/jats:italic>\n                    . We conclude that appropriate doses of Vitamin K2 protect\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>C.\u2009elegans<\/jats:italic>\n                    <\/jats:styled-content>\n                    from senescence by activating the JNK\u20101\/SIR\u20102.1\/DAF\u201016\u2010mediated anti\u2010mitochondrial oxidative stress pathway. These findings suggest that Vitamin K2 may have beneficial effects on lifespan and mitochondrial health in\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>C.\u2009elegans<\/jats:italic>\n                    <\/jats:styled-content>\n                    , providing a basis for further investigation into its potential relevance for aging and age\u2010related diseases in more complex model systems.\n                  <\/jats:p>","DOI":"10.1111\/acel.70530","type":"journal-article","created":{"date-parts":[[2026,5,3]],"date-time":"2026-05-03T21:02:37Z","timestamp":1777842157000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Vitamin K2 Extends Lifespan by Alleviating Mitochondrial Stress via the\n                    <scp>JNK<\/scp>\n                    \u20101\/\n                    <scp>SIR<\/scp>\n                    \u20102.1\/\n                    <scp>DAF<\/scp>\n                    \u201016 Signaling Axis in\n                    <i>Caenorhabditis elegans<\/i>"],"prefix":"10.1111","volume":"25","author":[{"given":"Song\u2010Yu","family":"Guo","sequence":"first","affiliation":[{"name":"Department of Physiology, College of Basic Medical Sciences, Liaoning Provincial Key Laboratory of Cerebral Diseases Dalian Medical University  Dalian Liaoning China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu\u2010Qi","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Physiology, College of Basic Medical Sciences, Liaoning Provincial Key Laboratory of Cerebral Diseases Dalian Medical University  Dalian Liaoning China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hua","family":"Piao","sequence":"additional","affiliation":[{"name":"Department of Physiology, College of Basic Medical Sciences, Liaoning Provincial Key Laboratory of Cerebral Diseases Dalian Medical University  Dalian 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