{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T03:33:46Z","timestamp":1791516826301,"version":"4.3.4"},"reference-count":51,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2026,7,3]],"date-time":"2026-07-03T00:00:00Z","timestamp":1783036800000},"content-version":"vor","delay-in-days":2,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001654","name":"German Academic Exchange Service London","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001654","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Aging Cell"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>The intestine is one of the first organs to show signs of aging, including cellular changes, microbiota shifts, and reduced regenerative capacity. The different components of the gut\u2014such as the epithelium (which is directly exposed to a diverse array of host\u2013microbe interactions), the microbiota itself, and the underlying enteric nervous system\u2014likely contribute to aging in distinct ways. Understanding their individual and interactive roles is key to elucidating the mechanisms of intestinal aging. To better understand the contribution of individual components to intestinal aging, we analyzed gut tissue characteristics and compared these parameters with the composition and gene expression levels of colonic organoids by using two mouse strains: the aging\u2010resistant SAMR1 line and the SAMP8 line, which exhibit an accelerated aging phenotype. Here, we demonstrate that colonic organoids derived from these mice retain the age\u2010related characteristics of the colonic tissue, including changes in morphology and cellular composition. Furthermore, introducing the enteric nervous system into organoid culture revealed that the age of the epithelium exerts a more pronounced influence on the aging phenotype than the age of the innervating tissue. Interestingly, successfully delivering fecal extracts to organoids revealed that gut microbiota metabolites from aged animals resulted in an aging phenotype of the gut epithelium in\u00a0vitro. In summary, our findings indicate the impact of aging on the gut epithelium and its interplay with the nervous system and microbiota. This may in future provide new strategies for slowing the aging process in the gut by manipulating the gut commensals.<\/jats:p>","DOI":"10.1111\/acel.70608","type":"journal-article","created":{"date-parts":[[2026,7,3]],"date-time":"2026-07-03T07:15:49Z","timestamp":1783062949000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Interplay of the\n                    <scp>ENS<\/scp>\n                    and Microbiota With Murine Gut Epithelium\u2010Derived Organoids in Aging"],"prefix":"10.1111","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4676-3225","authenticated-orcid":false,"given":"Tinh Thi","family":"Nguyen","sequence":"first","affiliation":[{"name":"Department of Psychiatry and Psychotherapy University Medical Center Mainz  Mainz Germany"},{"name":"Chromatin Regulation Group Institute of Molecular Biology  Mainz Germany"},{"name":"Faculty of Computer Sciences and Microsystems Technology Kaiserslautern University of Applied Sciences  Kaiserslautern Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dennis","family":"Schapelhouman","sequence":"additional","affiliation":[{"name":"Department of Psychiatry and Psychotherapy University Medical Center Mainz  Mainz Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katharina","family":"Fischer","sequence":"additional","affiliation":[{"name":"Department of Psychiatry and Psychotherapy University Medical Center Mainz  Mainz Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jia\u2010Xuan","family":"Chen","sequence":"additional","affiliation":[{"name":"Institute of Molecular Biology  Mainz Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mario","family":"Dejung","sequence":"additional","affiliation":[{"name":"Institute of Molecular Biology  Mainz 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