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However, the cellular and molecular mechanisms underlying gingival aging remain unclear, hindering the development of targeted therapies. In this study, we performed the first single\u2010cell transcriptomic analysis of aging human gingiva, identifying primary cilia as potential regulators of gingival fibroblast senescence. We demonstrated that aged gingival tissues exhibit increased fibroblast senescence and enhanced ciliogenesis compared to young tissues. Suppression of ciliogenesis significantly reduced senescence markers and alleviated DNA damage in aged fibroblasts accompanied by increased AKT activation. In addition, FOXO1 inactivation and enhanced expression of DNA repair\u2010related genes were found after suppression of ciliogenesis in aged gingival fibroblasts. Importantly, inhibition of AKT partially reversed the anti\u2010senescent phenotypes induced by ciliogenesis suppression. Functionally, adeno\u2010associated virus\u2010mediated suppression of ciliogenesis in aged mice mitigated gingival fibroblast senescence, reduced inflammation, and diminished tissue fibrosis. These findings highlight that primary cilia may contribute to the regulation of gingival fibroblast senescence and identify ciliary dynamics and AKT signaling downstream of cilia as potential therapeutic targets for managing aging\u2010related gingival diseases, providing a potential strategy to improve periodontal health in the elderly.<\/jats:p>","DOI":"10.1111\/acel.70627","type":"journal-article","created":{"date-parts":[[2026,7,11]],"date-time":"2026-07-11T03:32:28Z","timestamp":1783740748000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Suppression of Ciliogenesis Alleviates Cellular Senescence via\n                    <scp>AKT<\/scp>\n                    Signaling in Gingival Aging"],"prefix":"10.1111","volume":"25","author":[{"given":"Wenjun","family":"Shao","sequence":"first","affiliation":[{"name":"State Key Laboratory of Oral &amp; Maxillofacial Reconstruction and Regeneration, Key Laboratory of Oral Biomedicine Ministry of Education, Hubei Key Laboratory of Stomatology, School &amp; Hospital of Stomatology Wuhan University  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huihui","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Nephrology, Wuhan Children's Hospital (Wuhan Maternal and Child Healthcare Center), Tongji Medical College Huazhong University of Science &amp; Technology  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chenghu","family":"Yin","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Oral &amp; Maxillofacial Reconstruction and Regeneration, Key Laboratory of Oral Biomedicine Ministry of Education, Hubei Key Laboratory of Stomatology, School &amp; Hospital of Stomatology Wuhan University  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yunjie","family":"Zhang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Algal 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