{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T13:27:06Z","timestamp":1790774826577,"version":"4.1.0"},"reference-count":52,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T00:00:00Z","timestamp":1785369600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T00:00:00Z","timestamp":1785369600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82574088"],"award-info":[{"award-number":["82574088"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004735","name":"Natural Science Foundation of Hunan Province","doi-asserted-by":"publisher","award":["2025JJ50654"],"award-info":[{"award-number":["2025JJ50654"]}],"id":[{"id":"10.13039\/501100004735","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Aging Cell"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Age\u2010related sarcopenia is characterized by a progressive decline in skeletal muscle mass and function, with satellite cell dysfunction representing a central pathogenic mechanism. Diosgenin, a steroidal saponin derived from plants of the\n                    <jats:italic>Dioscorea<\/jats:italic>\n                    genus, has demonstrated potential anti\u2010aging properties; however, its role in sarcopenia remains unclear. In this study, naturally aged C57BL\/6J mice and a D\u2010galactose (D\u2010gal)\u2013induced senescent C2C12 cell model were employed to systematically investigate the effects of diosgenin on muscle function, satellite cell dynamics, and the sirtuin 1 (SIRT1)\/peroxisome proliferator\u2010activated receptor gamma coactivator\u20101 alpha (PGC\u20101\u03b1) signaling pathway. Diosgenin treatment significantly improved forelimb grip strength and exercise endurance, increased the gastrocnemius muscle index, and enlarged muscle fiber cross\u2010sectional area in aged mice. Mechanistically, diosgenin upregulated the expression of myokines meteorin\u2010like protein (METRNL) and insulin\u2010like growth factor 1 (IGF\u20101) at both mRNA and protein levels, increased the number of proliferative satellite cells positive for paired box\u00a07 (Pax7) and Ki67, and enhanced the expression of myogenic markers, including myogenic factor 5 (Myf5), Pax7, and myosin heavy chain II (MyHC II). These effects were mediated by direct activation of SIRT1, leading to deacetylation of PGC\u20101\u03b1. Notably, pharmacological inhibition of SIRT1 with EX527 markedly abrogated the diosgenin\u2010induced effects. Molecular docking and cellular thermal shift assays further confirmed the direct interaction between diosgenin and SIRT1. Collectively, these findings demonstrate that diosgenin alleviates age\u2010related sarcopenia by activating the SIRT1\/PGC\u20101\u03b1 signaling pathway to promote satellite cell proliferation and myogenic differentiation, highlighting its potential as a promising therapeutic candidate for sarcopenia.\n                  <\/jats:p>","DOI":"10.1111\/acel.70651","type":"journal-article","created":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T04:26:24Z","timestamp":1785471984000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Diosgenin Alleviates Age\u2010Related Sarcopenia by Promoting Satellite Cell Proliferation and Myogenic Differentiation via Activation of the\n                    <scp>SIRT1<\/scp>\n                    \/\n                    <scp>PGC<\/scp>\n                    \u20101\u03b1 Signaling Pathway"],"prefix":"10.1111","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8403-7342","authenticated-orcid":false,"given":"Xin","family":"Zeng","sequence":"first","affiliation":[{"name":"Department of Nutrition Science and Food Hygiene, Xiangya School of Public Health Central South University  Changsha China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Han","family":"Ding","sequence":"additional","affiliation":[{"name":"Department of Nutrition Science and Food Hygiene, Xiangya School of Public Health Central South University  Changsha China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ziye","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Epidemiology and Health Statistics, Xiangya School of Public Health Central South University  Changsha China"},{"name":"Hunan Provincial Key Laboratory of Clinical Epidemiology, Xiangya School of Public Health Central South University  Changsha China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qian","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Life Science and Medicine Zhejiang Science and Technology University  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peiyao","family":"Guan","sequence":"additional","affiliation":[{"name":"Department of Nutrition Science and Food Hygiene, Xiangya School of Public Health Central South University  Changsha China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1689-7257","authenticated-orcid":false,"given":"Tingting","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Epidemiology and Health Statistics, Xiangya School of Public Health Central South University  Changsha China"},{"name":"Hunan Provincial Key Laboratory of Clinical Epidemiology, Xiangya School of Public Health Central South University  Changsha 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China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4578-5118","authenticated-orcid":false,"given":"Hong","family":"Qin","sequence":"additional","affiliation":[{"name":"Department of Nutrition Science and Food Hygiene, Xiangya School of Public Health Central South University  Changsha 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