{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T05:55:32Z","timestamp":1790574932446,"version":"4.1.0"},"reference-count":45,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T00:00:00Z","timestamp":1787184000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T00:00:00Z","timestamp":1787184000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001700","name":"Ministry of Education, Culture, Sports, Science and Technology","doi-asserted-by":"publisher","award":["JPMXP1323015486"],"award-info":[{"award-number":["JPMXP1323015486"]}],"id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Aging Cell"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>The clinical outcomes of trabeculectomy, a standard glaucoma filtration surgery (GFS), have dramatically improved with the introduction of mitomycin C (MMC), an antimetabolite widely used to inhibit postoperative scarring. MMC exerts anti\u2010scarring effects primarily by promoting apoptosis and suppressing fibroblast proliferation at the surgical site. However, despite these beneficial effects, MMC also induces the production of inflammatory cytokines, and its full mechanism of action remains incompletely understood. Moreover, a subset of patients continues to experience poor surgical outcomes even after MMC application, highlighting the need for complementary therapeutic strategies. Comprehensive transcriptomic analysis of postoperative GFS tissues revealed that MMC promoted the expression of genes associated with cellular senescence and inflammatory responses. Supporting this, in\u00a0vitro experiments suggested that the transcriptional changes were associated with MMC\u2010induced senescence\u2010associated secretory phenotype (SASP)\u2010like changes in the fibroblasts. Single\u2010cell RNA sequencing (scRNA\u2010Seq) analyses of postoperative GFS tissues also revealed that fibroblasts after GFS with MMC secrete SASP factors. Furthermore, Cell\u2013Cell communication analysis identified CCL7 as one of the key molecules involved in inflammatory cell migration and the promotion of fibrosis. Importantly, we demonstrated that fibroblasts exhibiting SASP\u2010like features can be selectively eliminated by a specific senolytic agent, suggesting a novel and promising therapeutic approach that may improve long\u2010term surgical outcomes after GFS.<\/jats:p>","DOI":"10.1111\/acel.70680","type":"journal-article","created":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T06:00:30Z","timestamp":1787292030000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Surviving Fibroblasts After Treatment With Mitomycin C Exhibit\n                    <scp>SASP<\/scp>\n                    \u2010Like Phenotype After Glaucoma Filtration Surgery"],"prefix":"10.1111","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-2050-8085","authenticated-orcid":false,"given":"Akitoshi","family":"Kimura","sequence":"first","affiliation":[{"name":"Department of Ophthalmology, Faculty of Life Sciences Kumamoto University  Kumamoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4867-1925","authenticated-orcid":false,"given":"Tomokazu","family":"Fujimoto","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Faculty of Life Sciences Kumamoto University  Kumamoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Satoshi","family":"Iraha","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Faculty of Life Sciences Kumamoto University  Kumamoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kei\u2010Ichiro","family":"Yasunaga","sequence":"additional","affiliation":[{"name":"Liaison Laboratory Research Promotion Center, Institute of Molecular Embryology and Genetics Kumamoto University  Kumamoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miyuki","family":"Inoue\u2010Mochita","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Faculty of Life Sciences Kumamoto University  Kumamoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naofumi","family":"Funagura","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Faculty of Life Sciences Kumamoto University  Kumamoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shunsuke","family":"Tanigawa","sequence":"additional","affiliation":[{"name":"Department of Kidney Development, Institute of Molecular Embryology and Genetics Kumamoto University  Kumamoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4728-0113","authenticated-orcid":false,"given":"Daisuke","family":"Kurotaki","sequence":"additional","affiliation":[{"name":"Laboratory of Chromatin Organization in Immune Cell Development, International Research Center for Medical Sciences Kumamoto University  Kumamoto Japan"},{"name":"Center for Metabolic Regulation of Healthy Aging, Graduate School of Medical Sciences Kumamoto University  Kumamoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomoaki","family":"Koga","sequence":"additional","affiliation":[{"name":"Department of Medical Cell Biology, Institute of Molecular Embryology and Genetics Kumamoto University  Kumamoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fumika","family":"Watanabe\u2010Kitamura","sequence":"additional","affiliation":[{"name":"Inomata Eye Clinic  Kumamoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Utako","family":"Tsutsumi\u2010Kuroda","sequence":"additional","affiliation":[{"name":"Inada Eye Clinic  Kumamoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2196-8673","authenticated-orcid":false,"given":"Mitsuyoshi","family":"Nakao","sequence":"additional","affiliation":[{"name":"Department of Medical Cell Biology, Institute of Molecular Embryology and Genetics Kumamoto University  Kumamoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Toshihiro","family":"Inoue","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Faculty of Life Sciences Kumamoto University  Kumamoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,20]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41591\u2010022\u201001923\u2010y"},{"key":"e_1_2_11_3_1","first-page":"172","article-title":"Enhanced Intraocular Pressure Controlling Effectiveness of Trabeculectomy by Local Application of Mitomycin\u2010C","volume":"9","author":"Chen C. 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