{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T19:46:25Z","timestamp":1792611985785,"version":"4.5.0"},"reference-count":67,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T00:00:00Z","timestamp":1790640000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T00:00:00Z","timestamp":1790640000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82030098"],"award-info":[{"award-number":["82030098"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82574083"],"award-info":[{"award-number":["82574083"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Aging Cell"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Age\u2010related megakaryocyte (MK) dysfunction disrupts platelet production and may increase thrombotic risk, but whether coenzyme Q10 (CoQ10) restores MK homeostasis through autophagy remains unclear. Male C57BL\/6J mice aged 1, 9, 15, 19, 21, or 23\u2009months received CoQ10 for 12\u2009weeks. Bone marrow MK proliferation, CD41\/CD61 expression, DNA polyploidy, autophagy\u2010associated proteins, and platelet phenotypes were evaluated. H\n                    <jats:sub>2<\/jats:sub>\n                    O\n                    <jats:sub>2<\/jats:sub>\n                    \u2010induced senescent MEG\u201001 cells were used for pharmacological modulation with rapamycin and LY294002, bafilomycin A1\u2010based autophagic flux assays, and COPS3 knockdown. CoQ10 enhanced MK proliferation, maturation, and polyploidization in aged mice. Changes in the LC3\u2010II\/LC3\u2010I ratio and p62 in bone marrow MKs were consistent with enhanced autophagy. Proteomic analysis identified increased COPS3 abundance in MKs from CoQ10\u2010treated 24\u2010month\u2010old mice. In senescent MEG\u201001 cells, responses to rapamycin and LY294002 supported the involvement of autophagy\u2010related signaling in the effects of CoQ10. CoQ10 increased COPS3 expression even under autophagy\u2010inhibitory conditions and produced greater bafilomycin A1\u2010sensitive LC3\u2010II accumulation, supporting increased autophagic flux. COPS3 knockdown attenuated CoQ10\u2010induced changes in autophagy\u2010associated markers and partially diminished its effects on MK maturation and polyploidization, indicating that COPS3 contributes to, but may not solely mediate, these responses. CoQ10 did not alter platelet counts in naturally aged mice but was associated with reduced platelet aggregation and activation. Together, these findings suggest that CoQ10 ameliorates age\u2010related MK dysfunction and platelet hyperreactivity, in part through COPS3\u2010associated autophagy regulation. These findings provide a mechanistic basis for further investigation of CoQ10 as a nutritional strategy for age\u2010related platelet dysfunction.\n                  <\/jats:p>","DOI":"10.1111\/acel.70726","type":"journal-article","created":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T12:15:02Z","timestamp":1790684102000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Dietary\n                    <scp>CoQ10<\/scp>\n                    Ameliorates Age\u2010Related Megakaryocyte Dysfunction via\n                    <scp>COPS3<\/scp>\n                    \u2010Mediated Activation of Autophagy"],"prefix":"10.1111","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6039-2267","authenticated-orcid":false,"given":"Yixuan","family":"Xu","sequence":"first","affiliation":[{"name":"School of Public Health (Shenzhen), Shenzhen Campus of Sun Yat\u2010Sen University, Sun Yat\u2010Sen University  Shenzhen 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