{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T04:49:11Z","timestamp":1791434951593,"version":"4.3.3"},"reference-count":44,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T00:00:00Z","timestamp":1791331200000},"content-version":"vor","delay-in-days":6,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Aging Cell"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Time\u2010restricted feeding (TRF) restricts food intake to daily windows, yet its efficacy against vascular aging and underlying mechanisms remain unclear. Here, we demonstrate that TRF attenuates angiotension II \u2010induced accelerated vascular aging by reshaping gut microbiota, stabilizing microbial diversity, and elevating the Firmicutes\/Bacteroidetes ratio. TRF preserved intestinal barrier integrity, upregulated tight junction proteins (Occludin, ZO\u20101, MUC2), reduced serum lipopolysaccharide, and suppressed systemic inflammation. These benefits translated into alleviated hypertension, aortic stiffness (reflected by pulse wave velocity), and pathological cardiac remodeling. Fecal microbiota transplantation recapitulated these effects, whereas antibiotic\u2010induced microbiota depletion abolished TRF protection; importantly, exogenous acetate supplementation fully restored the phenotypes, establishing acetic acid as the critical downstream mediator. Mechanistically, acetic acid bound to GPR43, inhibited HDAC3, increased H4K8ac acetylation, blocked NLRP3\u2010mediated pyroptosis, and reduced senescence markers (p53, p21, p16) in human umbilical vein endothelial cells, aorta, and heart. 16S rRNA sequencing and short \u2010 chain fatty acids metabolomic profiling further identified\n                    <jats:italic>Limosilactobacillus<\/jats:italic>\n                    as the primary acetic acid\u2010producing bacterium enriched by TRF. Together, we uncover a\n                    <jats:italic>Limosilactobacillus<\/jats:italic>\n                    \u2010acetic acid\u2010GPR43\/HDAC3\/H4K8ac\/NLRP3 axis, offering mechanistic insights into TRF as a non\u2010pharmacological strategy against age\u2010related cardiovascular diseases.\n                  <\/jats:p>","DOI":"10.1111\/acel.70728","type":"journal-article","created":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T03:51:33Z","timestamp":1791431493000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Time\u2010Restricted Feeding Delays Cardiovascular Aging by Increasing Acetic Acid Through Remodeling Gut Microbiota"],"prefix":"10.1111","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6184-4862","authenticated-orcid":false,"given":"Man","family":"Liu","sequence":"first","affiliation":[{"name":"Key Laboratory of Vascular Ageing, Ministry of Education, Department of Geriatrics, Tongji Hospital of Tongji Medical College Huazhong University of Science and Technology  Wuhan People's Republic of China"},{"name":"Department of Geriatrics, Tongji Hospital of Tongji Medical College Huazhong University of Science and Technology  Wuhan People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mandi","family":"Luo","sequence":"additional","affiliation":[{"name":"Key Laboratory of Vascular Ageing, Ministry of Education, Department of Geriatrics, Tongji Hospital of Tongji Medical College Huazhong University of Science and Technology  Wuhan People's Republic of China"},{"name":"Department of Geriatrics, Tongji Hospital of Tongji Medical College Huazhong University of Science and Technology  Wuhan People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yangyang","family":"Huang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Vascular Ageing, Ministry of Education, Department of Geriatrics, Tongji Hospital of Tongji Medical College Huazhong University of Science and Technology  Wuhan People's Republic of China"},{"name":"Department of Geriatrics, Tongji Hospital of Tongji Medical College Huazhong University of Science and Technology  Wuhan People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tianyi","family":"Ji","sequence":"additional","affiliation":[{"name":"Key Laboratory of Vascular Ageing, Ministry of Education, Department of Geriatrics, Tongji Hospital of Tongji Medical College Huazhong University of Science and Technology  Wuhan People's Republic of China"},{"name":"Department of Geriatrics, Tongji Hospital of Tongji Medical College Huazhong University of Science and Technology  Wuhan People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuman","family":"Wang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Vascular Ageing, Ministry of Education, Department of Geriatrics, Tongji Hospital of Tongji Medical College Huazhong University of Science and Technology  Wuhan People's Republic of China"},{"name":"Department of Geriatrics, Tongji Hospital of Tongji Medical College Huazhong University of Science and Technology  Wuhan People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qian","family":"Wang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Vascular Ageing, Ministry of Education, Department of Geriatrics, Tongji Hospital of Tongji Medical College Huazhong University of Science and Technology  Wuhan People's Republic of China"},{"name":"Department of Geriatrics, Tongji Hospital of Tongji Medical College Huazhong University of Science and Technology  Wuhan People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jie","family":"Huang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Vascular Ageing, Ministry of Education, Department of Geriatrics, Tongji Hospital of Tongji Medical College Huazhong University of Science and Technology  Wuhan People's Republic of China"},{"name":"Department of Geriatrics, Tongji Hospital of Tongji Medical College Huazhong University of Science and Technology  Wuhan People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5600-3548","authenticated-orcid":false,"given":"Tao","family":"Jiang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Vascular Ageing, Ministry of Education, Department of Geriatrics, Tongji Hospital of Tongji Medical College Huazhong University of Science and Technology  Wuhan People's Republic of China"},{"name":"Department of Geriatrics, Tongji Hospital of Tongji Medical College Huazhong University of Science and Technology  Wuhan People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9044-5032","authenticated-orcid":false,"given":"Cuntai","family":"Zhang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Vascular Ageing, Ministry of Education, Department of Geriatrics, Tongji Hospital of Tongji Medical College Huazhong University of Science and Technology  Wuhan People's Republic of China"},{"name":"Department of Geriatrics, Tongji Hospital of Tongji Medical College Huazhong University of Science and Technology  Wuhan People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tao","family":"Mi","sequence":"additional","affiliation":[{"name":"Key Laboratory of Vascular Ageing, Ministry of Education, Department of Geriatrics, Tongji Hospital of Tongji Medical College Huazhong University of Science and Technology  Wuhan People's Republic of China"},{"name":"Department of Geriatrics, Tongji Hospital of Tongji Medical College Huazhong University of Science and Technology  Wuhan People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mian","family":"Cheng","sequence":"additional","affiliation":[{"name":"Key Laboratory of Vascular Ageing, Ministry of Education, Department of Geriatrics, Tongji Hospital of Tongji Medical College Huazhong University of Science and Technology  Wuhan People's Republic of China"},{"name":"Department of Geriatrics, Tongji Hospital of Tongji Medical College Huazhong University of Science and Technology  Wuhan People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,7]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2022.04.016"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010018\u201036941\u20109"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2020.06.018"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2022.12.006"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1080\/19490976.2021.1994835"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1080\/19490976.2024.2390164"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/nutd.2014.23"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2015.02.020"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1256682"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.03399\u201020"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2023.106036"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1161\/circulationaha.106.653980"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jproteome.8b00536"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1080\/19490976.2025.2486515"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.arr.2022.101706"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1161\/cir.0000000000001256"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.cir.0000048892.83521.58"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.abe7365"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1039\/d0fo02890h"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0106184"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2022.11.001"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1080\/003655202317316105"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1093\/gerona\/glae214"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2024.1321395"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms23063303"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41577\u2010024\u201001014\u20108"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471\u20102180\u20109\u2010123"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11906\u2010023\u201001285\u2010x"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11357\u2010020\u201000156\u20106"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.arr.2008.12.002"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.1080\/19490976.2015.1134082"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41569\u2010018\u20100007\u2010y"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.1152\/physiolgenomics.00089.2016"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41579\u2010024\u201001068\u20104"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2018.04.010"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jacc.2019.03.024"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41569\u2010018\u20100108\u20107"},{"key":"e_1_2_11_39_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586\u2010021\u201003934\u20100"},{"key":"e_1_2_11_40_1","doi-asserted-by":"publisher","DOI":"10.1161\/circresaha.107.168369"},{"key":"e_1_2_11_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jacc.2019.11.061"},{"key":"e_1_2_11_42_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbmr.2701"},{"key":"e_1_2_11_43_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10620\u2010012\u20102259\u20104"},{"key":"e_1_2_11_44_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2024.05.014"},{"key":"e_1_2_11_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2019.11.004"}],"container-title":["Aging Cell"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/acel.70728","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/acel.70728","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/acel.70728","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T03:51:34Z","timestamp":1791431494000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/acel.70728"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10]]},"references-count":44,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2026,10]]}},"alternative-id":["10.1111\/acel.70728"],"URL":"https:\/\/doi.org\/10.1111\/acel.70728","archive":["Portico"],"relation":{},"ISSN":["1474-9718","1474-9726"],"issn-type":[{"value":"1474-9718","type":"print"},{"value":"1474-9726","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,10]]},"assertion":[{"value":"2025-11-13","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-12","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-10-07","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70728"}}