{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T05:05:31Z","timestamp":1790744731553,"version":"4.1.0"},"reference-count":57,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T00:00:00Z","timestamp":1790640000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T00:00:00Z","timestamp":1790640000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82271596"],"award-info":[{"award-number":["82271596"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82501916"],"award-info":[{"award-number":["82501916"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Aging Cell"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Aging is accompanied by progressive immune remodeling and functional decline across multiple organs. However, the role of neutrophils and their heterogeneous states in these processes remains incompletely understood. Here we integrated single\u2010cell RNA sequencing datasets from multiple organs of young and aged mice to characterize aging\u2010associated neutrophil remodeling. We identified organ\u2010dependent changes in neutrophil abundance and state composition, together with neutrophil subsets enriched for inflammatory, oxidative stress, complement, and IL\u20106\u2010related transcriptional programs. In\u00a0vitro, exposure to conditioned media from neutrophils isolated from aged mice or IL\u20106\u2010stimulated neutrophil\u2010like cells increased inflammatory and senescence\u2010associated responses in vascular smooth muscle and hepatocyte\u2010derived cells. Bone marrow single\u2010cell analysis further revealed aging\u2010associated shifts in progenitor composition and transcriptional states, including an old\u2010enriched HPC11 population characterized by enhanced myeloid priming. Silico perturbation analysis nominated FGF didate regulators of HPC subtype\u2010specific transcriptional programs. Together, our study provides a multi\u2010organ single\u2010cell resource for defining neutrophil remodeling during aging and highlights candidate neutrophil states and potential signaling axes associated with age\u2010related inflammatory tissue environments.<\/jats:p>","DOI":"10.1111\/acel.70735","type":"journal-article","created":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T04:52:56Z","timestamp":1790743976000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Single\u2010Cell Mapping Identifies Aging\u2010Associated Neutrophil Remodeling Across Multiple Organs"],"prefix":"10.1111","volume":"25","author":[{"given":"Yuman","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Geriatrics, Tongji Hospital, Tongji Medical College Huazhong University of Science and Technology  Wuhan China"},{"name":"Key Laboratory of Vascular Aging, Ministry of Education, Tongji Hospital, Tongji Medical College Huazhong University of Science and Technology  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-5393-6325","authenticated-orcid":false,"given":"Teding","family":"Chang","sequence":"additional","affiliation":[{"name":"Department of Traumatic Surgery, Tongji Trauma Center, Tongji Hospital, Tongji Medical College Huazhong University of Science and Technology  Wuhan China"},{"name":"Department of Surgery University of Pittsburgh  Pittsburgh Pennsylvania USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-8430-5780","authenticated-orcid":false,"given":"Yangguang","family":"Fu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Vascular Aging, Ministry of Education, Tongji Hospital, Tongji Medical College Huazhong University of Science and Technology  Wuhan China"},{"name":"Experimental Medicine Center Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0138-6916","authenticated-orcid":false,"given":"Guangyu","family":"Liang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Vascular Aging, Ministry of Education, Tongji Hospital, Tongji Medical College Huazhong University of Science and Technology  Wuhan China"},{"name":"Experimental Medicine Center Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huicheng","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2010Head and Neck Surgery, Tongji Hospital, Tongji Medical College Huazhong University of Science and Technology  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7730-9266","authenticated-orcid":false,"given":"Le","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Geriatrics, Tongji Hospital, Tongji Medical College Huazhong University of Science and Technology  Wuhan China"},{"name":"Key Laboratory of Vascular Aging, Ministry of Education, Tongji Hospital, Tongji Medical College Huazhong University of Science and Technology  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9672-3016","authenticated-orcid":false,"given":"Jinhua","family":"Yan","sequence":"additional","affiliation":[{"name":"Department of Geriatrics, Tongji Hospital, Tongji Medical College Huazhong University of Science and Technology  Wuhan China"},{"name":"Key Laboratory of Vascular Aging, Ministry of Education, Tongji Hospital, Tongji Medical College Huazhong University of Science and Technology  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6184-4862","authenticated-orcid":false,"given":"Man","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Geriatrics, Tongji Hospital, Tongji Medical College Huazhong University of Science and Technology  Wuhan China"},{"name":"Key Laboratory of Vascular Aging, Ministry of Education, Tongji Hospital, Tongji Medical College Huazhong University of Science and Technology  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4667-4572","authenticated-orcid":false,"given":"Anding","family":"Liu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Vascular Aging, Ministry of Education, Tongji Hospital, Tongji Medical College Huazhong University of Science and Technology  Wuhan China"},{"name":"Experimental Medicine Center Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9044-5032","authenticated-orcid":false,"given":"Cuntai","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Geriatrics, Tongji Hospital, Tongji Medical College Huazhong University of Science and Technology  Wuhan China"},{"name":"Key Laboratory of Vascular Aging, Ministry of Education, Tongji Hospital, Tongji Medical College Huazhong University of Science and Technology  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,29]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2019.01.002"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.4463"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood\u20102007\u201004\u2010087213"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586\u2010021\u201003795\u20107"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/aps.2013.92"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood\u20102016\u201009\u2010687822"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2021.04.025"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1210\/endrev\/bnad008"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41592\u2010019\u20100667\u20105"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010021\u201027150\u20106"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood\u20102010\u201005\u2010283564"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586\u2010019\u20100969\u2010x"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2020.01259"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010023\u201043900\u20100"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.21037\/tcr\u201021\u201045"},{"issue":"6","key":"e_1_2_10_17_1","doi-asserted-by":"crossref","first-page":"2099","DOI":"10.1182\/blood.V91.6.2099","article-title":"Interleukin\u20106 Production by Human Neutrophils After Fc\u2010Receptor Cross\u2010Linking or Exposure to Granulocyte Colony\u2010Stimulating Factor","volume":"91","author":"Ericson S. G.","year":"1998","journal-title":"Blood"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.181.3.2189"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41423\u2010020\u201000608\u20101"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40164\u2010015\u20100024\u2010z"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/s1097\u20102765(00)00071\u2010x"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41590\u2010023\u201001505\u20101"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cyto.2011.01.007"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/s43587\u2010024\u201000694\u20100"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrclinonc.2018.8"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2025.03.011"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.15252\/embj.2020106048"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI15555"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2023.03.005"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2122716119"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41392\u2010023\u201001451\u20102"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm3434"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni.2641"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.patter.2022.100434"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri.2017.105"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12979\u2010016\u20100076\u2010x"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20242154"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018\u2010010\u20100433\u20104"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1002\/JLB.2RU1021\u2010573R"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586\u2010018\u20100590\u20104"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.abl4896"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41419\u2010022\u201005377\u20105"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41569\u2010020\u20100431\u20107"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.120.317200"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.1111\/sji.12413"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.1111\/imr.13153"},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2020.02063"},{"key":"e_1_2_10_48_1","doi-asserted-by":"publisher","DOI":"10.1161\/ATVBAHA.117.309580"},{"key":"e_1_2_10_49_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41368\u2010024\u201000332\u2010w"},{"key":"e_1_2_10_50_1","doi-asserted-by":"publisher","DOI":"10.1002\/jlb.67.1.40"},{"key":"e_1_2_10_51_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41577\u2010022\u201000787\u20100"},{"key":"e_1_2_10_52_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41590\u2010020\u20100736\u2010z"},{"key":"e_1_2_10_53_1","doi-asserted-by":"publisher","DOI":"10.1172\/jci.insight.187072"},{"key":"e_1_2_10_54_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41392\u2010024\u201002049\u2010y"},{"key":"e_1_2_10_55_1","doi-asserted-by":"publisher","DOI":"10.1038\/s44161\u2010024\u201000561\u20106"},{"key":"e_1_2_10_56_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms7061"},{"key":"e_1_2_10_57_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep19674"},{"key":"e_1_2_10_58_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00011\u2010023\u201001753\u20109"}],"container-title":["Aging Cell"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/acel.70735","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/acel.70735","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/acel.70735","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T04:53:03Z","timestamp":1790743983000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/acel.70735"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,29]]},"references-count":57,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2026,10]]}},"alternative-id":["10.1111\/acel.70735"],"URL":"https:\/\/doi.org\/10.1111\/acel.70735","archive":["Portico"],"relation":{},"ISSN":["1474-9718","1474-9726"],"issn-type":[{"value":"1474-9718","type":"print"},{"value":"1474-9726","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,29]]},"assertion":[{"value":"2026-01-05","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-15","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-29","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70735"}}