{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T19:46:12Z","timestamp":1792611972244,"version":"4.5.0"},"reference-count":46,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100000069","name":"National Institute of Arthritis and Musculoskeletal and Skin Diseases","doi-asserted-by":"publisher","award":["R01 AR078764"],"award-info":[{"award-number":["R01 AR078764"]}],"id":[{"id":"10.13039\/100000069","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Aging Cell"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Aging exacerbates the incidence of discogenic chronic low back pain by excessive inflammation and immune cell presence. Aging reduces intervertebral disc (IVD) Wnt signaling, which is associated with IVD cell differentiation, IVD cell proliferation and anti\u2010inflammatory signaling. However, it is unclear how in vivo Wnt signaling\u2010absent IVD cells, which model aged IVD cells, regulate chemotactic signaling and immune cell recruitment. We hypothesized that Wnt signaling\u2010absent IVD cells attenuate chemotactic transcription and immune cell recruitment to naive IVD and in response to IVD injury. First, because aging reduces Wnt signaling in all IVD cells, we deleted\n                    <jats:italic>\u03b2\u2010catenin<\/jats:italic>\n                    using the AcanCreERT2.\n                    <jats:italic>\u03b2\u2010catenin<\/jats:italic>\n                    deletion reduced monocyte chemoattractant protein\u20101 (MCP1\/CCL2)\n                    <jats:italic>Ccl2<\/jats:italic>\n                    in male and female IVDs by 58% and 79%, respectively (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u20095\u20136\/sex). Using positron emission tomography\/computed tomography (PET\/CT) imaging of myeloid cells in 5\u2010month\u2010old mice (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u20094 IVD\/sex\/grp), deletion of\n                    <jats:italic>\u03b2\u2010catenin<\/jats:italic>\n                    reduced myeloid\u2010derived cell burden in naive IVD by 79% and attenuated IVD injury\u2010induced recruitment by 48%. Next, because nucleus pulposus (NP) cells begin losing Wnt signaling early in life, we deleted\n                    <jats:italic>\u03b2\u2010catenin<\/jats:italic>\n                    using the ShhCreERT2. Compared to RNA\u2010sequencing of WT IVD post injury (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u20093\/grp), deletion of\n                    <jats:italic>\u03b2\u2010catenin<\/jats:italic>\n                    in NP cells impaired the activation of immune cell pathways, such as MCP1\/CCL2. Together, the chemotactic transcriptomic response and myeloid cell accumulation due to IVD injury were impaired by the absence of IVD Wnt signaling transcription cofactor\n                    <jats:italic>\u03b2\u2010catenin<\/jats:italic>\n                    . These data suggest that low\u2010grade IVD inflammation and chronic IVD immune cell accumulation in aged IVD cells are regulated by IVD Wnt signaling.\n                  <\/jats:p>","DOI":"10.1111\/acel.70740","type":"journal-article","created":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T06:53:45Z","timestamp":1790924025000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["<i>\u03b2\u2010Catenin<\/i>\n                    \u2010Deficient Intervertebral Disc Cells Reduce Chemotactic Transcription and Myeloid Cell Recruitment to Injured Discs of Mice"],"prefix":"10.1111","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0380-1850","authenticated-orcid":false,"given":"Tori M.","family":"Kroon","sequence":"first","affiliation":[{"name":"Department of Orthopedics Icahn School of Medicine at Mount Sinai  New York New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9173-2666","authenticated-orcid":false,"given":"Jazz","family":"Munitz","sequence":"additional","affiliation":[{"name":"Biomedical Engineering and Imaging Institute Icahn School of Medicine at Mount Sinai  New York New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Ranzenigo","sequence":"additional","affiliation":[{"name":"Biomedical Engineering and Imaging Institute Icahn School of Medicine at Mount Sinai  New York New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"Umali","sequence":"additional","affiliation":[{"name":"Biomedical Engineering and Imaging Institute Icahn School of Medicine at Mount Sinai  New York New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-3844-2281","authenticated-orcid":false,"given":"William","family":"Wang","sequence":"additional","affiliation":[{"name":"Biomedical Engineering and Imaging Institute Icahn School of Medicine at Mount Sinai  New York New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-5863-089X","authenticated-orcid":false,"given":"Jonathan J.","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Orthopedics Icahn School of Medicine at Mount Sinai  New York New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0401-8262","authenticated-orcid":false,"given":"Abraham J. P.","family":"Teunissen","sequence":"additional","affiliation":[{"name":"Biomedical Engineering and Imaging Institute Icahn School of Medicine at Mount Sinai  New York New York USA"},{"name":"Icahn School of Medicine at Mount Sinai Cardiovascular Research Institute  New York New York USA"},{"name":"Icahn School of Medicine at Mount Sinai Icahn Genomics Institute  New York New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8446-845X","authenticated-orcid":false,"given":"Nilsson","family":"Holguin","sequence":"additional","affiliation":[{"name":"Department of Orthopedics Icahn School of Medicine at Mount Sinai  New York New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,1]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.brs.0000231761.73859.2c"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.2147\/COPD.S276792"},{"issue":"3","key":"e_1_2_9_4_1","first-page":"403","article-title":"Abnormal Magnetic\u2010Resonance Scans of the Lumbar Spine in Asymptomatic Subjects. 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S.Broz L.Jing et\u00a0al.2024.\u201cThe Progression of Neurovascular Features and Chemokine Signatures of the Intervertebral Disc With Degeneration.\u201dbioRxiv.https:\/\/doi.org\/10.1101\/2024.07.12.603182.","DOI":"10.1101\/2024.07.12.603182"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jot.2016.05.012"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13018\u2010025\u201006250\u20109"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.critrevonc.2025.104971"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1097\/00007632\u2010199904150\u201000005"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0098444"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.prp.2021.153366"}],"container-title":["Aging Cell"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/acel.70740","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/acel.70740","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/acel.70740","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T19:20:00Z","timestamp":1792610400000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/acel.70740"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10]]},"references-count":46,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2026,10]]}},"alternative-id":["10.1111\/acel.70740"],"URL":"https:\/\/doi.org\/10.1111\/acel.70740","archive":["Portico"],"relation":{},"ISSN":["1474-9718","1474-9726"],"issn-type":[{"value":"1474-9718","type":"print"},{"value":"1474-9726","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,10]]},"assertion":[{"value":"2026-03-06","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-17","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-10-01","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70740"}}