{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T06:46:28Z","timestamp":1792046788352,"version":"4.4.0"},"reference-count":30,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T00:00:00Z","timestamp":1791936000000},"content-version":"vor","delay-in-days":13,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["AG063944"],"award-info":[{"award-number":["AG063944"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["AG088465"],"award-info":[{"award-number":["AG088465"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["AG093185"],"award-info":[{"award-number":["AG093185"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["GM155433"],"award-info":[{"award-number":["GM155433"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["AR081367"],"award-info":[{"award-number":["AR081367"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["GM156679"],"award-info":[{"award-number":["GM156679"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000965","name":"American Federation for Aging Research","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000965","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Aging Cell"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Aged skeletal muscle has a reduced ability to adapt to hypertrophic stimuli concomitant with dysregulation across several molecular layers. Whether altered RNA splicing associates with impaired adaptive potential is not well studied. We hypothesized that aging disrupts the splicing response to involuntary muscle mechanical loading. Using deep short\u2010read Illumina RNA\u2010sequencing (SRS, 60 million read pairs) and third generation Oxford Nanopore long\u2010read RNA\u2010sequencing (LRS) combined with a murine model of acute muscle mechanical overload (MOV), we illustrate how aged muscle has a reduced or possibly delayed potential for isoform switching in response to stress. SRS identified hundreds of genes with isoform switching events in adult MOV relative to sham but only 37 in aged MOV. LRS identified 19 genes (including\n                    <jats:italic>Cacnb1, Naca, Psap<\/jats:italic>\n                    ) with true isoform switching in adult MOV and none in aged MOV. A subset of genes showed significant isoform switching without corresponding changes in overall gene expression, suggesting post\u2010transcriptional regulation in response to MOV. These data highlight the importance of analyzing the transcriptome at the isoform level to uncover transcript diversity and \u201cunseen genes\u201d that may have unique functions and mechanisms influencing hypertrophic adaptations.\n                  <\/jats:p>","DOI":"10.1111\/acel.70761","type":"journal-article","created":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T06:18:27Z","timestamp":1792045107000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A Hypertrophic Stimulus Drives Age\u2010Dependent Transcript Isoform Switching With and Without Differential Gene Expression in Skeletal Muscle"],"prefix":"10.1111","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4797-5265","authenticated-orcid":false,"given":"Pieter J.","family":"Koopmans","sequence":"first","affiliation":[{"name":"Cell and Molecular Biology Graduate Program University of Arkansas  Fayetteville Arkansas USA"},{"name":"Molecular Muscle Mass Regulation (M3R) Laboratory, Department of Health, Human Performance, &amp; Recreation University of Arkansas  Fayetteville Arkansas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abbey L.","family":"Stokes","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering University of Arkansas  Fayetteville Arkansas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8423-8854","authenticated-orcid":false,"given":"Christopher E.","family":"Nelson","sequence":"additional","affiliation":[{"name":"Cell and Molecular Biology Graduate Program University of Arkansas  Fayetteville Arkansas USA"},{"name":"Department of Biomedical Engineering University of Arkansas  Fayetteville Arkansas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3210-1629","authenticated-orcid":false,"given":"Yuan","family":"Wen","sequence":"additional","affiliation":[{"name":"Department of Physiology, College of Medicine University of Kentucky  Lexington Kentucky USA"},{"name":"Department of Internal Medicine, College of Medicine University of Kentucky  Lexington Kentucky USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4025-3945","authenticated-orcid":false,"given":"Ahmed","family":"Ismaeel","sequence":"additional","affiliation":[{"name":"Department of Anatomy, Physiology, and Pharmacology, College of Veterinary Medicine Auburn University  Auburn Alabama USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2783-7137","authenticated-orcid":false,"given":"Kevin A.","family":"Murach","sequence":"additional","affiliation":[{"name":"Cell and Molecular Biology Graduate Program University of Arkansas  Fayetteville Arkansas USA"},{"name":"Molecular Muscle Mass Regulation (M3R) Laboratory, Department of Health, Human Performance, &amp; Recreation University of Arkansas  Fayetteville Arkansas USA"},{"name":"NextGen Precision Health Initiative University of Missouri  Columbia Missouri USA"},{"name":"Department of Nutrition and Exercise Physiology University of Missouri  Columbia Missouri USA"},{"name":"Department of Medical Pharmacology and Physiology University of Missouri  Columbia Missouri USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,14]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1101\/gr.133744.111"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2018.08.083"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejcb.2011.10.004"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.RA117.000265"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2015.06.002"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/acel.12646"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1080\/10985549.2026.2627235"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11357\u2010022\u201000542\u20102"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/s43587\u2010022\u201000189\u2010w"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.35.22782"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010024\u201055607\u2010x"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.3390\/jal3020013"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/s44319\u2010025\u201000413\u20109"},{"key":"e_1_2_12_15_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood\u20102013\u201008\u2010521252"},{"key":"e_1_2_12_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/advs.202521633"},{"key":"e_1_2_12_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbc.2022.102515"},{"key":"e_1_2_12_18_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12863\u2010026\u201001440\u20108"},{"key":"e_1_2_12_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010025\u201060049\u20100"},{"key":"e_1_2_12_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/cdd.2014.173"},{"key":"e_1_2_12_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00109\u2010026\u201002643\u20103"},{"key":"e_1_2_12_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010020\u201015171\u20106"},{"key":"e_1_2_12_23_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201403021"},{"key":"e_1_2_12_24_1","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.aaw1131"},{"key":"e_1_2_12_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010021\u201022168\u20102"},{"key":"e_1_2_12_26_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.49874"},{"key":"e_1_2_12_27_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells14151210"},{"key":"e_1_2_12_28_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btz247"},{"key":"e_1_2_12_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2006.09.008"},{"key":"e_1_2_12_30_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.1318305"},{"key":"e_1_2_12_31_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.10.14.1763"}],"container-title":["Aging 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