{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,5]],"date-time":"2025-10-05T16:52:38Z","timestamp":1759683158341},"reference-count":38,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2014,4,1]],"date-time":"2014-04-01T00:00:00Z","timestamp":1396310400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Alcoholism Clin &amp;amp; Exp Res"],"published-print":{"date-parts":[[2014,5]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Studies indicate that exposure to ethanol (<jats:styled-content style=\"fixed-case\">E<\/jats:styled-content>t<jats:styled-content style=\"fixed-case\">OH<\/jats:styled-content>) during fetal development damages cerebellar <jats:styled-content style=\"fixed-case\">P<\/jats:styled-content>urkinje cells (<jats:styled-content style=\"fixed-case\">PC<\/jats:styled-content>s). <jats:styled-content style=\"fixed-case\">PC<\/jats:styled-content> proximal dendrites receive glutamatergic input from climbing fibers (<jats:styled-content style=\"fixed-case\">CF<\/jats:styled-content>s) originating at the inferior olive. <jats:styled-content style=\"fixed-case\">CF<\/jats:styled-content> input produces a characteristic response in <jats:styled-content style=\"fixed-case\">PC<\/jats:styled-content>s known as the complex spike (<jats:styled-content style=\"fixed-case\">CS<\/jats:styled-content>). During the first 2\u00a0weeks of life in rodents (equivalent to the human third trimester of pregnancy), <jats:styled-content style=\"fixed-case\">CF<\/jats:styled-content>\u2013<jats:styled-content style=\"fixed-case\">PC<\/jats:styled-content> synapses undergo profound refinement. Here, we characterized the impact of Et<jats:styled-content style=\"fixed-case\">OH<\/jats:styled-content> exposure during this period on <jats:styled-content style=\"fixed-case\">CF<\/jats:styled-content>\u2010evoked responses in <jats:styled-content style=\"fixed-case\">PC<\/jats:styled-content>s.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Using vapor chambers, neonatal rat pups and their mothers were exposed to air or Et<jats:styled-content style=\"fixed-case\">OH<\/jats:styled-content> for 4\u00a0h\/d between postnatal day 2 (<jats:styled-content style=\"fixed-case\">P<\/jats:styled-content>2) and <jats:styled-content style=\"fixed-case\">P<\/jats:styled-content>12 (pup serum Et<jats:styled-content style=\"fixed-case\">OH<\/jats:styled-content> concentration, 0.16\u00a0g\/dl). The function of <jats:styled-content style=\"fixed-case\">CF<\/jats:styled-content>\u2013<jats:styled-content style=\"fixed-case\">PC<\/jats:styled-content> synapses was characterized using patch\u2010clamp electrophysiological techniques in acute slices from the cerebellar vermis. Experiments were performed soon after <jats:styled-content style=\"fixed-case\">E<\/jats:styled-content>t<jats:styled-content style=\"fixed-case\">OH<\/jats:styled-content> withdrawal, when perisomatic <jats:styled-content style=\"fixed-case\">CF<\/jats:styled-content>s are still being eliminated (<jats:styled-content style=\"fixed-case\">P<\/jats:styled-content>15 to <jats:styled-content style=\"fixed-case\">P<\/jats:styled-content>17), and after weaning when <jats:styled-content style=\"fixed-case\">CF<\/jats:styled-content> dendritic translocation is almost complete (<jats:styled-content style=\"fixed-case\">P<\/jats:styled-content>21 to <jats:styled-content style=\"fixed-case\">P<\/jats:styled-content>34).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Neither the baseline characteristics of the <jats:styled-content style=\"fixed-case\">CS<\/jats:styled-content> (<jats:styled-content style=\"fixed-case\">N<\/jats:styled-content>a<jats:sup>+<\/jats:sup> spike amplitude, area, coastline index, and afterhyperpolarization [<jats:styled-content style=\"fixed-case\">AHP<\/jats:styled-content>] amplitude) nor the type\u20101 metabotropic glutamate receptor (<jats:styled-content style=\"fixed-case\">mG<\/jats:styled-content>lu<jats:styled-content style=\"fixed-case\">R<\/jats:styled-content>1)\u2010mediated component of both the <jats:styled-content style=\"fixed-case\">CS<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">AHP<\/jats:styled-content> were significantly affected by <jats:styled-content style=\"fixed-case\">E<\/jats:styled-content>t<jats:styled-content style=\"fixed-case\">OH<\/jats:styled-content> exposure at <jats:styled-content style=\"fixed-case\">P<\/jats:styled-content>15 to <jats:styled-content style=\"fixed-case\">P<\/jats:styled-content>17 or <jats:styled-content style=\"fixed-case\">P<\/jats:styled-content>21 to <jats:styled-content style=\"fixed-case\">P<\/jats:styled-content>34. The m<jats:styled-content style=\"fixed-case\">G<\/jats:styled-content>lu<jats:styled-content style=\"fixed-case\">R<\/jats:styled-content>1\u2010dependent long\u2010term depression (<jats:styled-content style=\"fixed-case\">LTD<\/jats:styled-content>) of <jats:styled-content style=\"fixed-case\">CF<\/jats:styled-content>\u2010evoked excitatory postsynaptic currents was not significantly affected by <jats:styled-content style=\"fixed-case\">E<\/jats:styled-content>t<jats:styled-content style=\"fixed-case\">OH<\/jats:styled-content> exposure at <jats:styled-content style=\"fixed-case\">P<\/jats:styled-content>21 to <jats:styled-content style=\"fixed-case\">P<\/jats:styled-content>34.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Et<jats:styled-content style=\"fixed-case\">OH<\/jats:styled-content> exposure during the third trimester equivalent neither affected basal characteristics of the <jats:styled-content style=\"fixed-case\">CS<\/jats:styled-content> nor <jats:styled-content style=\"fixed-case\">CF<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">LTD<\/jats:styled-content> at rat cerebellar <jats:styled-content style=\"fixed-case\">PC<\/jats:styled-content>s from juvenile rats.<\/jats:p><\/jats:sec>","DOI":"10.1111\/acer.12362","type":"journal-article","created":{"date-parts":[[2014,4,1]],"date-time":"2014-04-01T19:33:03Z","timestamp":1396380783000},"page":"1293-1300","source":"Crossref","is-referenced-by-count":12,"title":["Third Trimester\u2010Equivalent Ethanol Exposure Does Not Alter Complex Spikes and Climbing Fiber Long\u2010Term Depression in Cerebellar Purkinje Neurons from Juvenile Rats"],"prefix":"10.1111","volume":"38","author":[{"given":"Paula A.","family":"Zamudio\u2010Bulcock","sequence":"first","affiliation":[{"name":"Department of Neurosciences School of Medicine University of New Mexico Health Sciences Center Albuquerque New Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Russell A.","family":"Morton","sequence":"additional","affiliation":[{"name":"Department of Neurosciences School of Medicine University of New Mexico Health Sciences Center Albuquerque New Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C. 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