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Based on observations that ethanol (EtOH) altered intracellular mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s during development, we tested the hypothesis that plasma mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s were biomarkers for maternal alcohol exposure, and for past in utero exposure, in the neonate.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Pregnant sheep were exposed to a binge model of EtOH consumption resulting in an average peak blood alcohol content of 243\u00a0mg\/dl, for a third\u2010trimester\u2010equivalent period from gestational day 4 (GD4) to GD132. Mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> profiles were assessed by quantitative <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> analysis in plasma, erythrocyte, and leukocytes obtained from nonpregnant ewes, and plasma from pregnant ewes 24\u00a0hours following the last binge EtOH episode, and from newborn lambs, at birth on ~<jats:styled-content style=\"fixed-case\">GD<\/jats:styled-content>147.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Pregnant ewe and newborn lamb <jats:sub><jats:italic>cir<\/jats:italic><\/jats:sub>mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> profiles were similar to each other and different from nonpregnant female plasma, erythrocyte, or leukocyte mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s. Significant changes in <jats:sub><jats:italic>cir<\/jats:italic><\/jats:sub>mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> profiles were observed in the EtOH\u2010exposed ewe and, at birth, in the in utero, EtOH\u2010exposed lamb. <jats:sub><jats:italic>Cir<\/jats:italic><\/jats:sub>mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s including miR\u20109, \u201015b, \u201019b, and \u201020a were sensitive and specific measures of EtOH exposure in both pregnant ewe and newborn lamb. Additionally, EtOH exposure altered guide\u2010to\u2010passenger strand <jats:sub><jats:italic>cir<\/jats:italic><\/jats:sub>mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> ratios in the pregnant ewe, but not in the lamb.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Shared profiles between pregnant dam and neonate suggest possible maternal\u2010fetal mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> transfer. <jats:sub><jats:italic>Cir<\/jats:italic><\/jats:sub>mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s are biomarkers for alcohol exposure during pregnancy, in both mother and neonate, and may constitute an important shared endocrine biomarker that is vulnerable to the maternal environment.<\/jats:p><\/jats:sec>","DOI":"10.1111\/acer.12378","type":"journal-article","created":{"date-parts":[[2014,3,3]],"date-time":"2014-03-03T16:48:41Z","timestamp":1393865321000},"page":"1390-1400","source":"Crossref","is-referenced-by-count":55,"title":["Maternal and Neonatal Plasma Micro<scp>RNA<\/scp> Biomarkers for Fetal Alcohol Exposure in an Ovine Model"],"prefix":"10.1111","volume":"38","author":[{"given":"Sridevi","family":"Balaraman","sequence":"first","affiliation":[{"name":"Department of Neuroscience and Experimental Therapeutics, and Women's Health in Neuroscience Program Texas A&amp;M Health Science Center College of Medicine Bryan Texas"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E. 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