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Alcohol inhibits <jats:styled-content style=\"fixed-case\">L<\/jats:styled-content>1\u2010mediated cell\u2013cell adhesion in diverse cell types and inhibits <jats:styled-content style=\"fixed-case\">L<\/jats:styled-content>1\u2010mediated neurite outgrowth in cerebellar granule neurons (<jats:styled-content style=\"fixed-case\">CGN<\/jats:styled-content>s). A recent report indicates that ethanol (<jats:styled-content style=\"fixed-case\">E<\/jats:styled-content>t<jats:styled-content style=\"fixed-case\">OH<\/jats:styled-content>) induces the translocation of <jats:styled-content style=\"fixed-case\">L<\/jats:styled-content>1 into <jats:styled-content style=\"fixed-case\">CGN<\/jats:styled-content> lipid rafts and that disruption of lipid rafts prevents <jats:styled-content style=\"fixed-case\">E<\/jats:styled-content>t<jats:styled-content style=\"fixed-case\">OH<\/jats:styled-content> inhibition of <jats:styled-content style=\"fixed-case\">L<\/jats:styled-content>1\u2010mediated neurite outgrowth. The same butanol\u2013pentanol cutoff was noted for alcohol\u2010induced translocation of <jats:styled-content style=\"fixed-case\">L<\/jats:styled-content>1 into lipid rafts that was reported previously for alcohol inhibition of <jats:styled-content style=\"fixed-case\">L<\/jats:styled-content>1 adhesion, suggesting that <jats:styled-content style=\"fixed-case\">E<\/jats:styled-content>t<jats:styled-content style=\"fixed-case\">OH<\/jats:styled-content> might inhibit <jats:styled-content style=\"fixed-case\">L<\/jats:styled-content>1 adhesion by shifting <jats:styled-content style=\"fixed-case\">L<\/jats:styled-content>1 into lipid rafts.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The <jats:styled-content style=\"fixed-case\">NIH<\/jats:styled-content>\/3<jats:styled-content style=\"fixed-case\">T<\/jats:styled-content>3 cell line, 2<jats:styled-content style=\"fixed-case\">A<\/jats:styled-content>2\u2010<jats:styled-content style=\"fixed-case\">L<\/jats:styled-content>1<jats:sub>s<\/jats:sub>, is a well\u2010characterized <jats:styled-content style=\"fixed-case\">E<\/jats:styled-content>t<jats:styled-content style=\"fixed-case\">OH<\/jats:styled-content>\u2010sensitive clonal cell line that stably expresses human <jats:styled-content style=\"fixed-case\">L<\/jats:styled-content>1. Cells were treated with 25\u00a0mM <jats:styled-content style=\"fixed-case\">E<\/jats:styled-content>t<jats:styled-content style=\"fixed-case\">OH<\/jats:styled-content>, 5\u00a0<jats:italic>\u03bc<\/jats:italic>M filipin, or both. Lipid rafts were enriched in membrane fractions by preparation of detergent\u2010resistant membrane (DRMs) fractions. Caveolin\u20101 was used as a marker of lipid rafts, and <jats:styled-content style=\"fixed-case\">L<\/jats:styled-content>1 and <jats:styled-content style=\"fixed-case\">S<\/jats:styled-content>rc were quantified by <jats:styled-content style=\"fixed-case\">W<\/jats:styled-content>estern blotting in lipid\u2010raft\u2010enriched membrane fractions and by immunohistochemistry.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>EtOH (25\u00a0mM) increased the percentage of <jats:styled-content style=\"fixed-case\">L<\/jats:styled-content>1, but not <jats:styled-content style=\"fixed-case\">S<\/jats:styled-content>rc, in 2<jats:styled-content style=\"fixed-case\">A<\/jats:styled-content>2\u2010<jats:styled-content style=\"fixed-case\">L<\/jats:styled-content>1<jats:sub>s<\/jats:sub> membrane fractions enriched in lipid rafts. Filipin, an agent known to disrupt lipid rafts, decreased the percentage of caveolin and <jats:styled-content style=\"fixed-case\">L<\/jats:styled-content>1 in <jats:styled-content style=\"fixed-case\">DRM<\/jats:styled-content>s from 2<jats:styled-content style=\"fixed-case\">A<\/jats:styled-content>2\u2010<jats:styled-content style=\"fixed-case\">L<\/jats:styled-content>1<jats:sub>s<\/jats:sub> cells. Filipin also blocked <jats:styled-content style=\"fixed-case\">E<\/jats:styled-content>t<jats:styled-content style=\"fixed-case\">OH<\/jats:styled-content>\u2010induced translocation of <jats:styled-content style=\"fixed-case\">L<\/jats:styled-content>1 into lipid rafts from 2<jats:styled-content style=\"fixed-case\">A<\/jats:styled-content>2\u2010<jats:styled-content style=\"fixed-case\">L<\/jats:styled-content>1<jats:sub>s<\/jats:sub> cells but did not significantly affect <jats:styled-content style=\"fixed-case\">L<\/jats:styled-content>1 adhesion or <jats:styled-content style=\"fixed-case\">E<\/jats:styled-content>t<jats:styled-content style=\"fixed-case\">OH<\/jats:styled-content> inhibition of <jats:styled-content style=\"fixed-case\">L<\/jats:styled-content>1 adhesion.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>These findings indicate that <jats:styled-content style=\"fixed-case\">E<\/jats:styled-content>t<jats:styled-content style=\"fixed-case\">OH<\/jats:styled-content> does not inhibit <jats:styled-content style=\"fixed-case\">L<\/jats:styled-content>1 adhesion in <jats:styled-content style=\"fixed-case\">NIH<\/jats:styled-content>\/3<jats:styled-content style=\"fixed-case\">T<\/jats:styled-content>3 cells by inducing the translocation of <jats:styled-content style=\"fixed-case\">L<\/jats:styled-content>1 into lipid rafts.<\/jats:p><\/jats:sec>","DOI":"10.1111\/acer.12556","type":"journal-article","created":{"date-parts":[[2014,11,24]],"date-time":"2014-11-24T15:41:43Z","timestamp":1416843703000},"page":"2707-2711","source":"Crossref","is-referenced-by-count":9,"title":["Effect of Lipid Raft Disruption on Ethanol Inhibition of <scp>L<\/scp>1 Adhesion"],"prefix":"10.1111","volume":"38","author":[{"given":"Xiaowei","family":"Dou","sequence":"first","affiliation":[{"name":"VA Boston Healthcare System Harvard Medical School West Roxbury 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