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One mode of EtOH\u2010induced interference in the normal developmental program appears to be through induction of apoptotic processes mediated by the Bcl\u20102 family of survival\u2010regulatory proteins. The present series of studies investigated the role of the Bcl\u20102\u2010related, pro\u2010apoptotic Bid protein, and its truncated, apoptotically active fragment, <jats:styled-content style=\"fixed-case\">tB<\/jats:styled-content>id, in developmental EtOH neurotoxicity.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Protein analyses were made via enzyme\u2010linked immunosorbent assays (ELISA) in neonatal rat cerebellum, of basal Bid, and of Bid and <jats:styled-content style=\"fixed-case\">tB<\/jats:styled-content>id, following EtOH exposure via vapor inhalation, at an age of peak EtOH sensitivity in this region (postnatal day 4 [P4]) and a later age of relative resistance (P7). ELISA analyses were also made of Bax:<jats:styled-content style=\"fixed-case\">tB<\/jats:styled-content>id heterodimers, a process which activates Bax, essential for its apoptotic functioning. Finally, in vitro assessments of the importance of <jats:styled-content style=\"fixed-case\">tB<\/jats:styled-content>id to EtOH neurotoxicity were made in cultured cerebellar granule cells, using a specific <jats:styled-content style=\"fixed-case\">tB<\/jats:styled-content>id inhibitor.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Basal levels of Bid were higher at P4 compared to P7, possibly contributing to the differential sensitivity. EtOH exposure elicited further increases in cytosolic Bid and mitochondrial <jats:styled-content style=\"fixed-case\">tB<\/jats:styled-content>id when administration was at P4, but not at P7. Bax:<jats:styled-content style=\"fixed-case\">tB<\/jats:styled-content>id heterodimers were markedly increased by EtOH exposure on P4, an increase which persisted even 2\u00a0hours after termination of treatment. Similar effects were not seen at P7. The in vitro analyses revealed that <jats:styled-content style=\"fixed-case\">tB<\/jats:styled-content>id inhibition provided complete protection against EtOH\u2010induced cell death and depressed EtOH\u2010mediated cytochrome\u2010c release.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>These results suggest that Bid\/<jats:styled-content style=\"fixed-case\">tB<\/jats:styled-content>id may be important elements in EtOH\u2010mediated neurotoxicity during CNS development. The molecular processes and interactions revealed may represent critical points which can be targeted in studies concerned with designing possible therapeutic strategies for minimizing these devastative effects.<\/jats:p><\/jats:sec>","DOI":"10.1111\/acer.12603","type":"journal-article","created":{"date-parts":[[2015,1,26]],"date-time":"2015-01-26T16:49:47Z","timestamp":1422290987000},"page":"55-63","source":"Crossref","is-referenced-by-count":9,"title":["Differential Effects of Ethanol on Bid, <scp>tB<\/scp>id, and Bax:<scp>tB<\/scp>id Interactions in Postnatal Day 4 and Postnatal Day 7 Rat Cerebellum"],"prefix":"10.1111","volume":"39","author":[{"given":"Marieta B.","family":"Heaton","sequence":"first","affiliation":[{"name":"Department of Neuroscience University of Florida College of Medicine Gainesville Florida"},{"name":"McKnight Brain Institute Gainesville Florida"},{"name":"Center for Addiction Research and Education Gainesville 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