{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,9]],"date-time":"2026-05-09T14:56:01Z","timestamp":1778338561588,"version":"3.51.4"},"reference-count":52,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2016,2,4]],"date-time":"2016-02-04T00:00:00Z","timestamp":1454544000000},"content-version":"vor","delay-in-days":3,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000027","name":"National Institute on Alcohol Abuse and Alcoholism","doi-asserted-by":"publisher","award":["AA019367"],"award-info":[{"award-number":["AA019367"]}],"id":[{"id":"10.13039\/100000027","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100008451","name":"Binghamton University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100008451","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Alcoholism Clin &amp;amp; Exp Res"],"published-print":{"date-parts":[[2016,2]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Ethanol is widely known for its depressant effects; however, the underlying neurobiological mechanisms are not clear. Calcium\u2010activated anion channels (<jats:styled-content style=\"fixed-case\">CAAC<\/jats:styled-content>s) contribute to extracellular chloride levels and thus may be involved in regulating inhibitory mechanisms within the central nervous system. Therefore, we hypothesized that<jats:styled-content style=\"fixed-case\">CAAC<\/jats:styled-content>s influence ethanol behavioral sensitivity by altering<jats:styled-content style=\"fixed-case\">CAAC<\/jats:styled-content>expression.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We assessed the role of<jats:styled-content style=\"fixed-case\">CAAC<\/jats:styled-content>s in ethanol\u2010induced loss of righting reflex (<jats:styled-content style=\"fixed-case\">LORR<\/jats:styled-content>) and locomotor activity using intracerebroventricular infusions of several nonselective<jats:styled-content style=\"fixed-case\">CAAC<\/jats:styled-content>blockers.<jats:styled-content style=\"fixed-case\">CAAC<\/jats:styled-content>expression was determined after ethanol exposure.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Ethanol\u2010induced<jats:styled-content style=\"fixed-case\">LORR<\/jats:styled-content>(4.0\u00a0g\/kg, intraperitoneally [i.p.]) was significantly attenuated by all 4<jats:styled-content style=\"fixed-case\">CAAC<\/jats:styled-content>blockers. Blocking<jats:styled-content style=\"fixed-case\">CAAC<\/jats:styled-content>s did not impact ethanol's low\u2010dose (1.5\u00a0g\/kg, i.p.) locomotor\u2010impairing effects. Biochemical analysis of<jats:styled-content style=\"fixed-case\">CAAC<\/jats:styled-content>protein expression revealed that cortical Bestrophin1 (Best1) and Tweety1 levels were reduced as early as 30\u00a0minutes following a single ethanol injection (3.5\u00a0g\/kg, intraperitoneally [i.p.]) and remained decreased 24\u00a0hours later in P2 fractions. Cortical Best1 levels were also reduced following 1.5\u00a0g\/kg. However,<jats:styled-content style=\"fixed-case\">CAAC<\/jats:styled-content>expression was unaltered in the striatum following a single ethanol exposure. Ethanol did not affect Tweety2 levels in either brain region.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>These results suggest that<jats:styled-content style=\"fixed-case\">CAAC<\/jats:styled-content>s are a major target of ethanol in\u00a0vivo<jats:italic>,<\/jats:italic>and the regulation of these channels contributes to select behavioral actions of ethanol.<\/jats:p><\/jats:sec>","DOI":"10.1111\/acer.12957","type":"journal-article","created":{"date-parts":[[2016,2,8]],"date-time":"2016-02-08T02:02:14Z","timestamp":1454896934000},"page":"301-308","source":"Crossref","is-referenced-by-count":6,"title":["Inhibitors of Calcium\u2010Activated Anion Channels Modulate Hypnotic Ethanol Responses in Adult Sprague Dawley Rats"],"prefix":"10.1111","volume":"40","author":[{"given":"Jenna M.","family":"Carter","sequence":"first","affiliation":[{"name":"Department of Psychology Center for Development and Behavioral Neuroscience Binghamton University \u2013 State University of New York Binghamton New York"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Justine D.","family":"Landin","sequence":"additional","affiliation":[{"name":"Department of Psychology Center for Development and Behavioral Neuroscience Binghamton University \u2013 State University of New York Binghamton New York"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eduardo D.","family":"Gigante","sequence":"additional","affiliation":[{"name":"Department of Psychology Center for Development and Behavioral Neuroscience Binghamton University \u2013 State University of New York Binghamton New York"},{"name":"Department of Health and Human Services Intramural Research Program National Institute on Drug Abuse National Institutes of Health Baltimore Maryland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Samuel P.","family":"Rieger","sequence":"additional","affiliation":[{"name":"Department of Psychology Center for Development and Behavioral Neuroscience Binghamton University \u2013 State University of New York Binghamton New York"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marvin R.","family":"Diaz","sequence":"additional","affiliation":[{"name":"Department of Psychology Center for Development and Behavioral Neuroscience Binghamton University \u2013 State University of New York Binghamton New York"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David F.","family":"Werner","sequence":"additional","affiliation":[{"name":"Department of Psychology Center for Development and Behavioral Neuroscience Binghamton University \u2013 State University of New York Binghamton New York"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,2,4]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1471-4159.2001.00097.x"},{"key":"e_1_2_6_3_1","first-page":"39","volume-title":"Methods of Behavior Analysis in Neuroscience","author":"Bailey KR","year":"2009"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/acer.12289"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0898-6568(94)00092-P"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(89)92149-9"},{"key":"e_1_2_6_7_1","doi-asserted-by":"crossref","first-page":"831","DOI":"10.1016\/S0026-895X(25)13076-9","article-title":"Effect of ethanol on intracellular ionized calcium concentrations in synaptosomes and hepatocytes","volume":"32","author":"Daniell LC","year":"1987","journal-title":"Mol Pharmacol"},{"key":"e_1_2_6_8_1","doi-asserted-by":"crossref","first-page":"875","DOI":"10.1016\/S0022-3565(25)22827-0","article-title":"Ethanol and inositol 1,4,5\u2010trisphosphate release calcium from separate stores of brain microsomes","volume":"250","author":"Daniell LC","year":"1989","journal-title":"J Pharmacol Exp Ther"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(03)00979-6"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1080\/13556210500352964"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/acer.12298"},{"key":"e_1_2_6_12_1","first-page":"148","article-title":"Bestrophin1 channels are insensitive to ethanol and do not mediate tonic GABAergic currents in cerebellar granule cells","volume":"5","author":"Diaz MR","year":"2011","journal-title":"Front Neurosci"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.4111-08.2009"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0082435"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1289\/ehp.9087199"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.alcohol.2014.01.011"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainres.2007.08.040"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1186\/1756-6606-6-4"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.physiol.67.032003.154341"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00022.2007"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-8993(98)00222-4"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1124\/jpet.107.126144"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1063-5823(02)53032-5"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2010.09.014"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1530-0277.1998.tb03611.x"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnins.2012.00044"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.16-08-02532.1996"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1184334"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.5284-13.2014"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1152\/jn.00565.2013"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0801068105"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(95)01456-X"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1742-7843.2012.00879.x"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1186\/1756-6606-5-38"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-3495(94)80712-X"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M002329200"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1186\/1756-6606-6-54"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.3193-09.2009"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1460-9568.2006.05298.x"},{"key":"e_1_2_6_40_1","first-page":"39","article-title":"Alcohol\u2010related morbidity and mortality","volume":"27","author":"Rehm J","year":"2003","journal-title":"Alcohol Res Health"},{"key":"e_1_2_6_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuint.2007.11.001"},{"key":"e_1_2_6_42_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00213-013-3267-6"},{"key":"e_1_2_6_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamem.2003.10.008"},{"key":"e_1_2_6_44_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2089"},{"key":"e_1_2_6_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbr.2006.05.010"},{"key":"e_1_2_6_46_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0056901"},{"key":"e_1_2_6_47_1","first-page":"144","article-title":"Alcohol and neurotransmitter interactions","volume":"21","author":"Valenzuela CF","year":"1997","journal-title":"Alcohol Health Res World"},{"key":"e_1_2_6_48_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1530-0277.2006.00220.x"},{"key":"e_1_2_6_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.alcohol.2009.09.036"},{"key":"e_1_2_6_50_1","doi-asserted-by":"publisher","DOI":"10.1002\/glia.10125"},{"key":"e_1_2_6_51_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1530-0277.2008.00832.x"},{"key":"e_1_2_6_52_1","doi-asserted-by":"publisher","DOI":"10.1186\/1756-6606-4-42"},{"key":"e_1_2_6_53_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2014.278754"}],"container-title":["Alcoholism: Clinical and Experimental Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Facer.12957","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/acer.12957","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,6,1]],"date-time":"2025-06-01T09:52:45Z","timestamp":1748771565000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/acer.12957"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,2]]},"references-count":52,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2016,2]]}},"alternative-id":["10.1111\/acer.12957"],"URL":"https:\/\/doi.org\/10.1111\/acer.12957","archive":["Portico"],"relation":{},"ISSN":["0145-6008","1530-0277"],"issn-type":[{"value":"0145-6008","type":"print"},{"value":"1530-0277","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,2]]}}}