{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T17:17:08Z","timestamp":1784567828100,"version":"3.55.0"},"reference-count":37,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2016,11,24]],"date-time":"2016-11-24T00:00:00Z","timestamp":1479945600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81270234"],"award-info":[{"award-number":["81270234"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Alcoholism Clin &amp;amp; Exp Res"],"published-print":{"date-parts":[[2017,1]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Abusive alcohol utilization of pregnant woman may cause congenital heart disease (<jats:styled-content style=\"fixed-case\">CHD<\/jats:styled-content>) of fetus, where alcohol ignites histone H3 hyperacetylation leading to abnormal development of heart morphogenesis and associated genes. Knowledge about the regularized upstream genes is little, but bone morphogenetic protein (<jats:styled-content style=\"fixed-case\">BMP<\/jats:styled-content>) signaling may actively and prominently take part in alteration in acetylation of histone H3. The supreme objective of this study was to unearth the involvement of <jats:styled-content style=\"fixed-case\">BMP<\/jats:styled-content> signaling pathway in alcohol\u2010driven hyperacetylation of histone H3 in cardiomyoblast cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Cardiomyoblast cells (H9c2 cells) were addicted with alcohol (100\u00a0mM) for 24\u00a0hours. Dorsomorphin (5\u00a0<jats:italic>\u03bc<\/jats:italic>M) was used for the inhibition of <jats:styled-content style=\"fixed-case\">BMP<\/jats:styled-content> signaling pathway. We detected the phosphorylation activity of <jats:styled-content style=\"fixed-case\">SMAD<\/jats:styled-content>1\/5\/8, <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> expression, histone acetyltransferases (<jats:styled-content style=\"fixed-case\">HAT<\/jats:styled-content>)\/histone deacetylase (<jats:styled-content style=\"fixed-case\">HDAC<\/jats:styled-content>) activity, and acetylation of histone H3.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Following alcohol exposure, phosphorylation of <jats:styled-content style=\"fixed-case\">SMAD<\/jats:styled-content>1\/5\/8 and <jats:styled-content style=\"fixed-case\">HAT<\/jats:styled-content> activities was increased to a significant extent, while histone H3 acetylation and expression of heart development\u2010related genes were also increased. The said phenomenon influenced by alcohol was reverted upon dorsomorphin treatment to the cells without effecting <jats:styled-content style=\"fixed-case\">HDAC<\/jats:styled-content> activity.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The data clearly identified that <jats:styled-content style=\"fixed-case\">BMP<\/jats:styled-content>\u2010mediated histone H3 acetylation of heart development\u2010related genes might be one of the possible cellular mechanisms to control alcohol\u2010induced expression of heart development\u2010related genes. Dorsomorphin, on the other hand, may modulate alcohol\u2010induced hyperacetylation of histone H3 through\u00a0<jats:styled-content style=\"fixed-case\">BMP<\/jats:styled-content> targeting, which could be a potential way to block <jats:styled-content style=\"fixed-case\">CHD<\/jats:styled-content>.<\/jats:p><\/jats:sec>","DOI":"10.1111\/acer.13273","type":"journal-article","created":{"date-parts":[[2016,11,24]],"date-time":"2016-11-24T12:52:49Z","timestamp":1479991969000},"page":"87-95","source":"Crossref","is-referenced-by-count":12,"title":["Alcohol Exposure Causes Overexpression of Heart Development\u2010Related Genes by Affecting the Histone H3 Acetylation via <scp>BMP<\/scp> Signaling Pathway in Cardiomyoblast Cells"],"prefix":"10.1111","volume":"41","author":[{"given":"Jin","family":"Shi","sequence":"first","affiliation":[{"name":"Heart Centre Children's Hospital of Chongqing Medical University Chongqing China"},{"name":"Key Laboratory of Developmental Disease in Childhood Ministry of Education Chongqing Medical University Chongqing China"},{"name":"Key Laboratory of Pediatrics in Chongqing Chongqing China"},{"name":"Chongqing International Science and Technology Cooperation Center for Child Development and Disorders Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weian","family":"Zhao","sequence":"additional","affiliation":[{"name":"Heart Centre Children's Hospital of Chongqing Medical University Chongqing China"},{"name":"Key Laboratory of Developmental Disease in Childhood Ministry of Education Chongqing Medical University Chongqing China"},{"name":"Key Laboratory of Pediatrics in Chongqing Chongqing China"},{"name":"Chongqing International Science and Technology Cooperation Center for Child Development and Disorders Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bo","family":"Pan","sequence":"additional","affiliation":[{"name":"Heart Centre Children's Hospital of Chongqing Medical 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