{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,17]],"date-time":"2026-06-17T13:30:42Z","timestamp":1781703042234,"version":"3.54.5"},"reference-count":25,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2017,1,12]],"date-time":"2017-01-12T00:00:00Z","timestamp":1484179200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81130008"],"award-info":[{"award-number":["81130008"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Alcoholism Clin &amp;amp; Exp Res"],"published-print":{"date-parts":[[2017,2]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Alcohol abuse incurs severe medical conditions, such as thrombocytopenia and hemorrhage, but the pathogenesis is not totally understood. Alcohol has been reported to induce apoptosis in eukaryotic cells, such as hepatocyte, nerve cell, corneal fibroblasts. However, it is still unclear whether alcohol induces platelet apoptosis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Washed human platelets were pretreated with ethanol (EtOH), and apoptotic events and platelet function were detected. In in\u00a0vivo experiments, C57<jats:styled-content style=\"fixed-case\">BL<\/jats:styled-content>\/6J mice were given EtOH by gavage. Platelet counts, tail bleeding time, and the stomach were examined.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>EtOH dose dependently induces depolarization of mitochondrial inner transmembrane potential, up\u2010regulation of Bax, down\u2010regulation of Bcl\u20102, and caspase\u20103 activation. EtOH does not induce surface expression of P\u2010selectin or <jats:styled-content style=\"fixed-case\">PAC<\/jats:styled-content>\u20101 binding, whereas significantly reduces collagen\u2010, thrombin\u2010, and <jats:styled-content style=\"fixed-case\">ADP<\/jats:styled-content>\u2010induced platelet aggregation. Moreover, EtOH induces c\u2010Jun <jats:styled-content style=\"fixed-case\">NH<\/jats:styled-content>2\u2010terminal kinase activation. In an in\u00a0vivo mouse model of the acute alcoholism, EtOH significantly reduces the number of circulating platelets, prolongs the tail bleeding time, and causes gastric mucosa hemorrhage.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>These data demonstrate that EtOH induces mitochondria\u2010mediated intrinsic platelet apoptosis, results in the reduction of the number of circulating platelets, and impairs in\u00a0vivo hemostasis. These findings reveal the possible pathogenesis of hemorrhagic symptoms in patients experiencing acute alcohol intoxication.<\/jats:p><\/jats:sec>","DOI":"10.1111\/acer.13295","type":"journal-article","created":{"date-parts":[[2017,1,13]],"date-time":"2017-01-13T03:27:34Z","timestamp":1484278054000},"page":"291-298","source":"Crossref","is-referenced-by-count":34,"title":["Ethanol Induces Platelet Apoptosis"],"prefix":"10.1111","volume":"41","author":[{"given":"Lei","family":"Liu","sequence":"first","affiliation":[{"name":"Jiangsu Institute of Hematology the First Affiliated Hospital of Soochow University Collaborative Innovation Center of Hematology Key Laboratory of Thrombosis and Hemostasis Ministry of Health Suzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mengxing","family":"Chen","sequence":"additional","affiliation":[{"name":"Jiangsu Institute of Hematology the First Affiliated Hospital of Soochow University Collaborative Innovation Center of Hematology Key Laboratory of Thrombosis and Hemostasis Ministry of Health Suzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lili","family":"Zhao","sequence":"additional","affiliation":[{"name":"Jiangsu Institute of Hematology the First Affiliated Hospital of Soochow University Collaborative Innovation Center of Hematology Key Laboratory of Thrombosis and Hemostasis Ministry of Health Suzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qing","family":"Zhao","sequence":"additional","affiliation":[{"name":"Jiangsu Institute of Hematology the First Affiliated Hospital of Soochow University Collaborative Innovation Center of Hematology Key Laboratory of Thrombosis and Hemostasis Ministry of Health Suzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Renping","family":"Hu","sequence":"additional","affiliation":[{"name":"Jiangsu Institute of Hematology the First Affiliated Hospital of Soochow University 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