{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,22]],"date-time":"2026-06-22T10:09:33Z","timestamp":1782122973006,"version":"3.54.5"},"reference-count":58,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2017,11,23]],"date-time":"2017-11-23T00:00:00Z","timestamp":1511395200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100006769","name":"Russian Science Foundation","doi-asserted-by":"publisher","award":["14\u201015\u201000392"],"award-info":[{"award-number":["14\u201015\u201000392"]}],"id":[{"id":"10.13039\/501100006769","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010249","name":"School of Medicine, Indiana University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100010249","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Alcoholism Clin &amp;amp; Exp Res"],"published-print":{"date-parts":[[2018,1]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Animal studies showed that alcoholic myopathy is characterized by the reduction in myofiber cross\u2010sectional area (<jats:styled-content style=\"fixed-case\">CSA<\/jats:styled-content>) and by impaired anabolic signaling. The goal of this study was to compare changes in <jats:styled-content style=\"fixed-case\">CSA<\/jats:styled-content> and fiber type composition with modifications in anabolic and catabolic signaling pathways at the early stages of alcohol misuse in humans.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Skeletal muscle samples from 7 male patients with chronic alcohol abuse (<jats:styled-content style=\"fixed-case\">AL<\/jats:styled-content>; 47.7\u00a0\u00b1\u00a02.0\u00a0years old; alcohol misuse duration 7.7\u00a0\u00b1\u00a00.6\u00a0years) were compared with muscle from a control group of 7 healthy men (C; 39.7\u00a0\u00b1\u00a05.0\u00a0years old). Biopsies from vastus lateralis muscles were taken and analyzed for the changes in fiber type composition, fiber <jats:styled-content style=\"fixed-case\">CSA<\/jats:styled-content>, and for the alterations in anabolic and catabolic signaling pathways.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">AL<\/jats:styled-content> patients did not have detectable clinical myopathy symptoms or muscle fiber atrophy, but the relative proportion of fast fibers was increased. There was a significant decrease in <jats:styled-content style=\"fixed-case\">IGF<\/jats:styled-content>\u20101 in plasma and <jats:styled-content style=\"fixed-case\">IRS<\/jats:styled-content>\u20101 protein content in muscle of <jats:styled-content style=\"fixed-case\">AL<\/jats:styled-content> group. Levels of total and phosphorylated p70S6K1, <jats:styled-content style=\"fixed-case\">GSK<\/jats:styled-content>3<jats:italic>\u03b2<\/jats:italic>, and p90<jats:styled-content style=\"fixed-case\">RSK<\/jats:styled-content>1 were not different between <jats:styled-content style=\"fixed-case\">AL<\/jats:styled-content> and C groups. Muscle of <jats:styled-content style=\"fixed-case\">AL<\/jats:styled-content> patients had increased <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> expression of <jats:styled-content style=\"fixed-case\">HSP<\/jats:styled-content>70 and <jats:styled-content style=\"fixed-case\">HSP<\/jats:styled-content>90. A marker of anabolic pathway p\u20104E\u2010<jats:styled-content style=\"fixed-case\">BP<\/jats:styled-content>1 was decreased, while catabolic markers (Mu<jats:styled-content style=\"fixed-case\">RF<\/jats:styled-content>\u20101, <jats:styled-content style=\"fixed-case\">MAF<\/jats:styled-content>bx, ubiquitinated proteins) were increased in <jats:styled-content style=\"fixed-case\">AL<\/jats:styled-content> patients when compared with C group.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>At the early stages of alcohol misuse in humans, changes in the regulation of anabolic and catabolic signaling pathways precede the development of skeletal muscle atrophy and manifestation of clinical symptoms of alcoholic myopathy.<\/jats:p><\/jats:sec>","DOI":"10.1111\/acer.13531","type":"journal-article","created":{"date-parts":[[2017,10,16]],"date-time":"2017-10-16T19:59:35Z","timestamp":1508183975000},"page":"41-52","source":"Crossref","is-referenced-by-count":18,"title":["Effect of Chronic Alcohol Abuse on Anabolic and Catabolic Signaling Pathways in Human Skeletal Muscle"],"prefix":"10.1111","volume":"42","author":[{"given":"Boris S.","family":"Shenkman","sequence":"first","affiliation":[{"name":"Institute of Biomedical Problems RAS Moscow Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Svetlana P.","family":"Belova","sequence":"additional","affiliation":[{"name":"Institute of Biomedical Problems RAS Moscow Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olga E.","family":"Zinovyeva","sequence":"additional","affiliation":[{"name":"Sechenov First Moscow State Medical University Russian Ministry of Health Moscow Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nudlya D.","family":"Samkhaeva","sequence":"additional","affiliation":[{"name":"Sechenov First Moscow State Medical University Russian Ministry of Health Moscow Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Timur M.","family":"Mirzoev","sequence":"additional","affiliation":[{"name":"Institute of Biomedical Problems RAS Moscow Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natalia A.","family":"Vilchinskaya","sequence":"additional","affiliation":[{"name":"Institute of Biomedical Problems RAS Moscow Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erzhena G.","family":"Altaeva","sequence":"additional","affiliation":[{"name":"Institute of Biomedical Problems RAS Moscow Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olga V.","family":"Turtikova","sequence":"additional","affiliation":[{"name":"Institute of Biomedical Problems RAS Moscow Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5863-7114","authenticated-orcid":false,"given":"Tatiana Y.","family":"Kostrominova","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Cell Biology Indiana University School of Medicine Northwest Gary Indiana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tatiana L.","family":"Nemirovskaya","sequence":"additional","affiliation":[{"name":"Institute of Biomedical Problems RAS Moscow Russia"},{"name":"Faculty of Basic Medicine Lomonosov Moscow State University Moscow Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,11,23]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0955-2863(03)00114-1"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.C200171200"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1113\/expphysiol.2009.050245"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phrs.2010.01.012"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.00736.2007"},{"key":"e_1_2_8_7_1","first-page":"543","article-title":"Relationship between ethanol\u2010related diseases and nutritional status in chronically alcoholic men","volume":"28","author":"Estruch R","year":"1993","journal-title":"Alcohol Alcohol"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1530-0277.2002.tb02493.x"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1080\/09595239600186021"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1053\/meta.2002.32803"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/aps.2010.161"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb0203-87"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1139\/H09-137"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.13009"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.34.9.991"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200403069"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/acer.12174"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12192-012-0375-x"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/emm.2016.81"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1236566"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbf.1740"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.3109\/00365517509095786"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1053\/meta.2002.28967"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.00083.2013"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.00181.2002"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2010.02.011"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.00177.2003"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1530-0277.2000.tb04614.x"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biocel.2005.04.013"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.00554.2003"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.00603.2006"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1357-2725(00)00081-9"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.12-204412"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1002\/mus.20743"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1385\/ENDO:29:1:73"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.metabol.2006.02.009"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1093\/ajcn\/78.2.326"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0026-0495(03)00063-5"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1002\/mus.20883"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0096378"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2010.203232"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.1113\/expphysiol.2009.050526"},{"key":"e_1_2_8_44_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0029665199001342"},{"key":"e_1_2_8_45_1","doi-asserted-by":"publisher","DOI":"10.1097\/MCO.0b013e328352b4c2"},{"key":"e_1_2_8_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0891-5849(01)00794-8"},{"key":"e_1_2_8_47_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1468-1331.2001.00303.x"},{"key":"e_1_2_8_48_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj2540631"},{"key":"e_1_2_8_49_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1996.tb00582.x"},{"key":"e_1_2_8_50_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1010336624154"},{"key":"e_1_2_8_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2004.08.026"},{"key":"e_1_2_8_52_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.abb.2015.07.011"},{"key":"e_1_2_8_53_1","first-page":"112","article-title":"Proteolytic signaling mechanisms in skeletal muscles in patients with alcohol\u2010induced muscle disease","volume":"39","author":"Shenkman BS","year":"2013","journal-title":"Fiziol Cheloveka"},{"key":"e_1_2_8_54_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.00006.2015"},{"key":"e_1_2_8_55_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2002.024935"},{"key":"e_1_2_8_56_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-59745-242-7_22"},{"key":"e_1_2_8_57_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.90862.2008"},{"key":"e_1_2_8_58_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2007.148338"},{"key":"e_1_2_8_59_1","unstructured":"World Health Organization(2000)International Guide for Monitoring Alcohol Consumption and Related Harm.Geneva Switzerland."}],"container-title":["Alcoholism: Clinical and Experimental Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Facer.13531","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/acer.13531","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,29]],"date-time":"2023-09-29T18:40:09Z","timestamp":1696012809000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/acer.13531"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,11,23]]},"references-count":58,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2018,1]]}},"alternative-id":["10.1111\/acer.13531"],"URL":"https:\/\/doi.org\/10.1111\/acer.13531","archive":["Portico"],"relation":{},"ISSN":["0145-6008","1530-0277"],"issn-type":[{"value":"0145-6008","type":"print"},{"value":"1530-0277","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,11,23]]}}}