{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T18:03:21Z","timestamp":1787162601566,"version":"build-2736575974"},"reference-count":52,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2017,12,4]],"date-time":"2017-12-04T00:00:00Z","timestamp":1512345600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["AA\u2010007216"],"award-info":[{"award-number":["AA\u2010007216"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Alcoholism Clin &amp; Exp Res"],"published-print":{"date-parts":[[2018,1]]},"abstract":"\n Background<\/jats:title>\n \n Alcohol (\n ALC<\/jats:styled-content>\n ) causes suppressed secretion of prepubertal luteinizing hormone\u2010releasing hormone (\n LHRH<\/jats:styled-content>\n ). Insulin\u2010like growth factor\u20101 (\n IGF<\/jats:styled-content>\n \u20101) and kisspeptin (Kp) are major regulators of\n LHRH<\/jats:styled-content>\n and are critical for puberty.\n IGF<\/jats:styled-content>\n \u20101 may be an upstream mediator of Kp in the preoptic area and rostral hypothalamic area (\n POA<\/jats:styled-content>\n \/\n RHA<\/jats:styled-content>\n ) of the rat brain, a region containing both Kp and\n LHRH<\/jats:styled-content>\n neurons. We investigated the ability of\n IGF<\/jats:styled-content>\n \u20101 to stimulate prepubertal Kp synthesis and release in\n POA<\/jats:styled-content>\n \/\n RHA<\/jats:styled-content>\n , and the potential inhibitory effects of\n ALC<\/jats:styled-content>\n .\n <\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n \n Immature female rats were administered either\n ALC<\/jats:styled-content>\n (3\u00a0g\/kg) or water via gastric gavage at 0730\u00a0hours. At 0900\u00a0hours, both groups were subdivided where half received either saline or\n IGF<\/jats:styled-content>\n \u20101 into the brain third ventricle. A second dose of\n ALC<\/jats:styled-content>\n (2\u00a0g\/kg) or water was administered at 1130\u00a0hours. Rats were killed 6\u00a0hours after injection and\n POA<\/jats:styled-content>\n \/\n RHA<\/jats:styled-content>\n region collected.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n IGF<\/jats:styled-content>\n \u20101 stimulated Kp, an action blocked by\n ALC<\/jats:styled-content>\n . Upstream to Kp,\n IGF<\/jats:styled-content>\n \u20101 receptor (\n IGF<\/jats:styled-content>\n \u20101R) activation, as demonstrated by the increase in insulin receptor substrate 1, resulted in activation of Akt, tuberous sclerosis 2, ras homologue enriched in brain, and mammalian target of rapamycin (\n mTOR<\/jats:styled-content>\n ).\n ALC<\/jats:styled-content>\n blocked the central action of\n IGF<\/jats:styled-content>\n \u20101 to induce their respective phosphorylation.\n IGF<\/jats:styled-content>\n \u20101 specificity and\n ALC<\/jats:styled-content>\n specificity for the Akt\u2010activated\n mTOR<\/jats:styled-content>\n pathway were demonstrated by the absence of effects on\n PRAS<\/jats:styled-content>\n 40. Furthermore,\n IGF<\/jats:styled-content>\n \u20101 stimulated Kp release from\n POA<\/jats:styled-content>\n \/\n RHA<\/jats:styled-content>\n incubated in\u00a0vitro.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusions<\/jats:title>\n \n IGF<\/jats:styled-content>\n \u20101 stimulates prepubertal Kp synthesis and release following activation of a\n mTOR<\/jats:styled-content>\n signaling pathway, and\n ALC<\/jats:styled-content>\n blocks this pathway at the level of\n IGF<\/jats:styled-content>\n \u20101R.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/acer.13539","type":"journal-article","created":{"date-parts":[[2017,10,26]],"date-time":"2017-10-26T09:39:49Z","timestamp":1509010789000},"page":"61-68","source":"Crossref","is-referenced-by-count":19,"title":["Regulation of Kisspeptin Synthesis and Release in the Preoptic\/Anterior Hypothalamic Region of Prepubertal Female Rats: Actions of\n IGF<\/scp>\n \u20101 and Alcohol"],"prefix":"10.1111","volume":"42","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9286-9229","authenticated-orcid":false,"given":"Jill K.","family":"Hiney","sequence":"first","affiliation":[{"name":"Department of Veterinary Integrative Biosciences College of Veterinary Medicine Texas A&M University College Station Texas"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vinod K.","family":"Srivastava","sequence":"additional","affiliation":[{"name":"Department of Veterinary Integrative Biosciences College of Veterinary Medicine Texas A&M University College Station Texas"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Danielle N.","family":"Vaden Anderson","sequence":"additional","affiliation":[{"name":"Department of Veterinary Integrative Biosciences College of Veterinary Medicine Texas A&M University College Station Texas"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicole 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