{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,9]],"date-time":"2026-03-09T10:53:59Z","timestamp":1773053639162,"version":"3.50.1"},"reference-count":33,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2017,12,18]],"date-time":"2017-12-18T00:00:00Z","timestamp":1513555200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81270544"],"award-info":[{"award-number":["81270544"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Alcoholism Clin &amp;amp; Exp Res"],"published-print":{"date-parts":[[2018,2]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Pancreatic fibrosis is a key pathological feature of alcoholic chronic pancreatitis (ACP). Bacterial endotoxin lipopolysaccharide (LPS) is considered as an important cofactor in the fibrogenesis of ACP. However, there are limitations in the use of exogenous LPS for evaluating the role of endotoxin in ACP pathogenesis. In this study, we determined the relationship between the concentration of LPS in the portal vein and pancreatic type I collagen (Col1) content in chronic alcohol\u2010fed rats.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Male Sprague Dawley rats were divided into 2 groups and fed with Lieber\u2013DeCarli isocaloric control (CON) liquid diet or ethanol (EtOH) (15\u00a0g\/kg\/d) liquid diet. Eleven CON or EtOH rats were euthanized at the end of week 8, 9, or 10. The plasma LPS from portal vein was determined. Pancreatic inflammatory injury and fibrosis were assessed. Pancreatic stellate cells (PSCs) and macrophages were identified; pancreatic type I collagen alpha 1 (Col1A1) and Toll\u2010like receptor (TLR4) mRNA and protein were examined; pancreatic chemokines and transforming growth factor\u2010beta1 (TGF\u2010<jats:italic>\u03b2<\/jats:italic>1) were determined.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Pancreatic inflammatory scores were increased in 10\u2010week EtOH rats compared with CON rats, but there was no significant difference in collagen deposition between 2 groups. The levels of portal vein LPS and pancreatic TLR4 and Col1A1 mRNA and protein were increased in a time\u2010dependent fashion in EtOH rats, with the highest levels occurring at 10\u00a0weeks. Additionally, by 8\u00a0weeks, pancreatic TLR4 and Col1A1 mRNA in EtOH rats were statistically increased as compared to CON rats, whereas portal vein LPS remained unchanged. The number of PSCs and macrophages and expression of chemokines (MCP\u20101, MIP\u20101<jats:italic>\u03b1,<\/jats:italic> and RANTES), TGF\u2010<jats:italic>\u03b2<\/jats:italic>1, or Col1A1 were significantly increased, each of which was positively correlated with the level of portal vein LPS in 10\u2010week EtOH rats.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>These results suggest that LPS is associated with alcohol\u2010induced fibrosis in pancreatitis and targeting of bacterial endotoxin may be a promising therapeutic strategy for ACP.<\/jats:p><\/jats:sec>","DOI":"10.1111\/acer.13550","type":"journal-article","created":{"date-parts":[[2017,11,9]],"date-time":"2017-11-09T19:06:36Z","timestamp":1510254396000},"page":"306-314","source":"Crossref","is-referenced-by-count":6,"title":["Role of Gut\u2010Derived Endotoxin on Type I Collagen Production in the Rat Pancreas After Chronic Alcohol Exposure"],"prefix":"10.1111","volume":"42","author":[{"given":"Hongyan","family":"Li","sequence":"first","affiliation":[{"name":"Department of Hepatic, Biliary Pancreatic Medicine First Hospital of Jilin University Changchun China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ming","family":"Xiu","sequence":"additional","affiliation":[{"name":"Department of Hepatic, Biliary Pancreatic Medicine First Hospital of Jilin University Changchun China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuhua","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Surgical Gastroenterolog First Hospital of Jilin University Changchun China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David R.","family":"Brigstock","sequence":"additional","affiliation":[{"name":"Research Institute at Nationwide Children's Hospital Columbus Ohio"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Li","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of Hepatic, Biliary Pancreatic Medicine First Hospital of Jilin University Changchun China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Limei","family":"Qu","sequence":"additional","affiliation":[{"name":"Department of Hepatic, Biliary Pancreatic Medicine First Hospital of Jilin University Changchun China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7148-6173","authenticated-orcid":false,"given":"Runping","family":"Gao","sequence":"additional","affiliation":[{"name":"Department of Hepatic, Biliary Pancreatic Medicine First Hospital of Jilin University Changchun China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,12,18]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2012.00344"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1097\/MOG.0000000000000196"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0096864"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0053028"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v23.i3.382"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.4291\/wjgp.v7.i1.48"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/modpathol.3800613"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1530-0277.2001.tb02359.x"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1136\/gutjnl-2011-301220"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2013.09.009"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajpath.2013.07.023"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-0436.1993.tb00705.x"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(14)60649-8"},{"issue":"2","key":"e_1_2_6_15_1","first-page":"S66:213","article-title":"The role of lipopolysaccharide on macrophage\u2010mediated inflammatory response in alcoholic chronic pancreatitis","volume":"109","author":"Li YJ","year":"2014","journal-title":"J Gastroenterol"},{"key":"e_1_2_6_16_1","first-page":"197","article-title":"Liquid diet technique of ethanol administration: 1989 update","volume":"24","author":"Lieber CS","year":"1989","journal-title":"Alcohol Alcohol"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12906-015-0789-y"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.4292\/wjgpt.v8.i1.10"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1136\/gut.2005.068593"},{"key":"e_1_2_6_20_1","doi-asserted-by":"crossref","first-page":"204","DOI":"10.1007\/s10620-008-0695-y","article-title":"Effects of ethanol and its metabolites on human pancreatic stellate cells","volume":"55","author":"Masamune A","year":"2011","journal-title":"Dig Dis Sci"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1159\/000129235"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI113300"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1499-3872(15)60415-5"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.23009"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1124\/jpet.108.148643"},{"key":"e_1_2_6_26_1","doi-asserted-by":"crossref","first-page":"s88","DOI":"10.14309\/00000434-201210001-00205","article-title":"Bacteria endotoxin: a trigger factor for human chronic pancreatitis","volume":"107","author":"Tian ZZ","year":"2012","journal-title":"Am J Gastroenterol"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1136\/gut.2010.211250"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.4291\/wjgp.v5.i2.82"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2007.06.062"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.2119\/molmed.2012.00260"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0054646"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1097\/MPA.0000000000000380"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms8158"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.24018"}],"container-title":["Alcoholism: Clinical and Experimental Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Facer.13550","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/acer.13550","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,27]],"date-time":"2023-09-27T20:28:43Z","timestamp":1695846523000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/acer.13550"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,12,18]]},"references-count":33,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2018,2]]}},"alternative-id":["10.1111\/acer.13550"],"URL":"https:\/\/doi.org\/10.1111\/acer.13550","archive":["Portico"],"relation":{},"ISSN":["0145-6008","1530-0277"],"issn-type":[{"value":"0145-6008","type":"print"},{"value":"1530-0277","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,12,18]]}}}