{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T00:34:16Z","timestamp":1747182856727,"version":"3.40.5"},"reference-count":34,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2019,5,23]],"date-time":"2019-05-23T00:00:00Z","timestamp":1558569600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100005047","name":"Natural Science Foundation of Liaoning Province","doi-asserted-by":"publisher","award":["20180530004"],"award-info":[{"award-number":["20180530004"]}],"id":[{"id":"10.13039\/501100005047","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81273343"],"award-info":[{"award-number":["81273343"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Alcoholism Clin &amp;amp; Exp Res"],"published-print":{"date-parts":[[2019,7]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>The Lieber\u2013DeCarli alcoholic liquid diet is a classical method for establishing animal models of alcoholic cardiomyopathy (ACM). No study has reported whether the AIN\u201093 diet, which is widely used as a standard diet for both long\u2010term and short\u2010term studies with laboratory animals, could be used to construct the ACM animal model. The present study intended to investigate whether the AIN\u201093 diet could be used to establish a mouse ACM model.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Twenty\u2010four C57BL\/6 male mice were randomly divided into 4 equally sized groups. In ethanol (EtOH)\u2010fed groups, mice were fed a 4%\u2010EtOH (w\/v, 28% of total calories) alcoholic liquid diet of Lieber\u2013DeCarli or the AIN\u201093 diet for chronic alcohol exposure for 180\u00a0days. In control\u2010fed groups, mice were fed with non\u2010EtOH liquid diets with the same calories as EtOH\u2010fed groups. Morphological observations of the hearts and molecular investigation of the brain natriuretic peptide (BNP) were carried out by echocardiography, hematoxylin and eosin (H&amp;E) and immunohistochemistry (IHC) staining, real\u2010time quantitative polymerase chain reaction (qPCR), and enzyme\u2010linked immunosorbent assay.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Echocardiography showed that mice fed with either the 4%\u2010EtOH Lieber\u2013DeCarli diet or the 4%\u2010EtOH AIN\u201093 diet had dilated ventricles and poor cardiac function. IHC staining of BNP, qPCR of BNP mRNA, and plasma concentration of BNP showed an up\u2010regulated expression in mice fed with both the 4%\u2010EtOH Lieber\u2013DeCarli and 4%\u2010EtOH AIN\u201093 diets. Less fatty liver was also observed in mice fed the AIN\u201093 alcoholic diet than those fed the Lieber\u2013DeCarli alcoholic diet.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The AIN\u201093 alcoholic liquid diet can be used to establish ACM animal models, as with the conventional Lieber\u2013DeCarli alcoholic liquid diet.<\/jats:p><\/jats:sec>","DOI":"10.1111\/acer.14069","type":"journal-article","created":{"date-parts":[[2019,5,1]],"date-time":"2019-05-01T01:21:46Z","timestamp":1556673706000},"page":"1452-1461","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["AIN\u201093 Diet as an Alternative Model to Lieber\u2013DeCarli Diet for Alcoholic Cardiomyopathy"],"prefix":"10.1111","volume":"43","author":[{"given":"Zhipeng","family":"Cao","sequence":"first","affiliation":[{"name":"Department of Forensic Pathology School of Forensic Medicine China Medical University Shenyang 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