{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T11:42:14Z","timestamp":1784547734536,"version":"3.55.0"},"reference-count":59,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2020,4,21]],"date-time":"2020-04-21T00:00:00Z","timestamp":1587427200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000027","name":"National Institute on Alcohol Abuse and Alcoholism","doi-asserted-by":"publisher","award":["AA021788"],"award-info":[{"award-number":["AA021788"]}],"id":[{"id":"10.13039\/100000027","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000027","name":"National Institute on Alcohol Abuse and Alcoholism","doi-asserted-by":"publisher","award":["AA021883"],"award-info":[{"award-number":["AA021883"]}],"id":[{"id":"10.13039\/100000027","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000027","name":"National Institute on Alcohol Abuse and Alcoholism","doi-asserted-by":"publisher","award":["AA021891"],"award-info":[{"award-number":["AA021891"]}],"id":[{"id":"10.13039\/100000027","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000027","name":"National Institute on Alcohol Abuse and Alcoholism","doi-asserted-by":"publisher","award":["U01 AA021840"],"award-info":[{"award-number":["U01 AA021840"]}],"id":[{"id":"10.13039\/100000027","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000027","name":"National Institute on Alcohol Abuse and Alcoholism","doi-asserted-by":"publisher","award":["UH2AA026218"],"award-info":[{"award-number":["UH2AA026218"]}],"id":[{"id":"10.13039\/100000027","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000865","name":"Bill and Melinda Gates Foundation","doi-asserted-by":"publisher","award":["OPP1035237"],"award-info":[{"award-number":["OPP1035237"]}],"id":[{"id":"10.13039\/100000865","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Alcoholism Clin &amp;amp; Exp Res"],"published-print":{"date-parts":[[2020,5]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Alcoholic hepatitis (AH) is a severe inflammatory liver disease that develops in some heavy drinkers. AH patients have intense hepatic infiltration of leukocytes. Up\u2010regulation of cell adhesion molecules (CAMs) upon endothelial cell (EC) activation plays an important role in leukocyte transendothelial migration. CAMs can shed from EC surface and accumulate in the blood, serving as soluble markers for EC activation. In this study, we examined the impact of heavy drinking on expression of soluble forms of EC activation markers (CD146, ICAM\u20101, VCAM\u20101, and VEGF\u2010A) and the effect of alcohol abstinence on the reversal of these abnormalities in heavy drinkers\u00a0with and without AH.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>ELISA and multiplex immunoassays were used to measure soluble EC activation\u00a0markers in plasma samples from 79 AH patients, 66 heavy drinkers without overt liver disease (HDC), and 44 healthy controls (HC) at baseline, 31 AH patients and 30 HDC at 6\u2010month follow\u2010up, and 18 AH patients and 25 HDC at 12\u2010month follow\u2010up.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>At baseline, the 4 soluble markers were significantly up\u2010regulated in AH patients compared with HDC and HC, whereas only sVCAM\u20101 was elevated in HDC relative to HC. At follow\u2010ups, plasma levels of CD146, VCAM\u20101, and VEGF\u2010A remained higher in AH patients, even for those who stopped drinking. These dysregulated markers correlated with AH disease severity, clinical parameters, and several soluble\u00a0inflammatory factors.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The levels of soluble CD146, ICAM\u20101, VCAM\u20101, and VEGF\u2010A were highly elevated in AH patients, and alcohol abstinence did not completely reverse these abnormalities.<\/jats:p><\/jats:sec>","DOI":"10.1111\/acer.14331","type":"journal-article","created":{"date-parts":[[2020,4,4]],"date-time":"2020-04-04T15:20:29Z","timestamp":1586013629000},"page":"1075-1087","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Persistent Hyperactivation of Endothelial Cells in Patients with Alcoholic Hepatitis"],"prefix":"10.1111","volume":"44","author":[{"given":"Ying","family":"Xia","sequence":"first","affiliation":[{"name":"Department of Clinical Laboratory the Second Affiliated Hospital and Yuying Children\u2019s Hospital of Wenzhou Medical University Wenzhou Zhejiang China"},{"name":"School of Laboratory Medicine and Life Sciences Wenzhou Medical University Wenzhou Zhejiang China"},{"name":"Department of Microbiology and Immunology Indiana University School of Medicine Indianapolis Indiana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Yang","sequence":"additional","affiliation":[{"name":"School of Laboratory Medicine and Life Sciences Wenzhou Medical University Wenzhou Zhejiang China"},{"name":"Department of Microbiology and Immunology Indiana University School of Medicine Indianapolis Indiana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arun J.","family":"Sanyal","sequence":"additional","affiliation":[{"name":"Division of Gastroenterology and Hepatology Department of Medicine Virginia Commonwealth University Richmond Virginia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vijay H.","family":"Shah","sequence":"additional","affiliation":[{"name":"Division of Gastroenterology and Hepatology Mayo Clinic Rochester Minnesota"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naga P.","family":"Chalasani","sequence":"additional","affiliation":[{"name":"Division of Gastroenterology and Hepatology Department of Medicine Indiana University School of Medicine Indianapolis Indiana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qigui","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology Indiana University School of Medicine Indianapolis Indiana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoqun","family":"Zheng","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory the Second Affiliated Hospital and Yuying Children\u2019s Hospital of Wenzhou Medical University Wenzhou Zhejiang China"},{"name":"School of Laboratory Medicine and Life Sciences Wenzhou Medical University Wenzhou Zhejiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4026-314X","authenticated-orcid":false,"given":"Wei","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology Indiana University School of Medicine Indianapolis Indiana"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,4,21]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.1840190308"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1136\/gutjnl-2011-301146"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms19061642"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2016.260471"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V98.13.3677"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1161\/ATVBAHA.108.183251"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.alcohol.2007.08.004"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0741-8329(02)00206-9"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/brain\/awp289"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v20.i20.6279"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaut.2011.09.003"},{"key":"e_1_2_7_13_1","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1002\/cyto.b.20502","article-title":"CD146+ T lymphocytes are increased in both the peripheral circulation and in the synovial effusions of patients with various musculoskeletal diseases and display pro\u2010inflammatory gene profiles","volume":"78","author":"Dagur PK","year":"2010","journal-title":"Cytometry B Clin Cytom"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v21.i42.11904"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1097\/HJH.0b013e328010929c"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2013.01.020"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2172-8-29"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2005-06-2307"},{"key":"e_1_2_7_19_1","first-page":"1623","article-title":"Platelet\u2010endothelial cell adhesion molecule\u20101 and CD146: soluble levels and in situ expression of cellular adhesion molecules implicated in the cohesion of endothelial cells in idiopathic inflammatory myopathies","volume":"33","author":"Figarella\u2010Branger D","year":"2006","journal-title":"J Rheumatol"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199001113220205"},{"key":"e_1_2_7_21_1","first-page":"725","article-title":"Expression of VCAM\u20101 and E\u2010selectin in an in vivo model of endothelial activation","volume":"143","author":"Fries JW","year":"1993","journal-title":"Am J Pathol"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2018.10.023"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.179.10.6673"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/cei.12151"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2009-06-229591"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0741-8329(02)00208-2"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.96.12.4219"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.24.15099"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M009705200"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/376517a0"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1582-4934.2009.00811.x"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1093\/brain\/aws212"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.29242"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.14309\/ctg.0000000000000052"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1172\/jci.insight.86660"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cgh.2016.05.041"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1096\/fasebj.6.8.1592209"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1111\/jdv.13905"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1471-4906(03)00117-0"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cpr.2004.08.003"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.3109\/00365521.2014.985706"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1111\/1523-1747.ep12471720"},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(90)90230-C"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.23258"},{"key":"e_1_2_7_45_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.158.5.2107"},{"key":"e_1_2_7_46_1","doi-asserted-by":"publisher","DOI":"10.1080\/17474124.2018.1481747"},{"key":"e_1_2_7_47_1","first-page":"665","article-title":"Expression of intercellular adhesion molecule\u20101 in atherosclerotic plaques","volume":"140","author":"Poston RN","year":"1992","journal-title":"Am J Pathol"},{"key":"e_1_2_7_48_1","doi-asserted-by":"crossref","first-page":"3788","DOI":"10.4049\/jimmunol.147.11.3788","article-title":"A form of circulating ICAM\u20101 in human serum","volume":"147","author":"Rothlein R","year":"1991","journal-title":"J Immunol"},{"key":"e_1_2_7_49_1","first-page":"113","article-title":"Current management of alcoholic hepatitis and future therapies","volume":"4","author":"Saberi B","year":"2016","journal-title":"J Clin Transl Hepatol"},{"key":"e_1_2_7_50_1","doi-asserted-by":"publisher","DOI":"10.1080\/13556210310001656376"},{"key":"e_1_2_7_51_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10157-007-0012-7"},{"key":"e_1_2_7_52_1","doi-asserted-by":"publisher","DOI":"10.1038\/ajg.2014.262"},{"key":"e_1_2_7_53_1","first-page":"1021","article-title":"Elevated vascular cell adhesion molecule\u20101 in AIDS encephalitis induced by simian immunodeficiency virus","volume":"141","author":"Sasseville VG","year":"1992","journal-title":"Am J Pathol"},{"key":"e_1_2_7_54_1","doi-asserted-by":"publisher","DOI":"10.1177\/039463201302600206"},{"key":"e_1_2_7_55_1","doi-asserted-by":"publisher","DOI":"10.1177\/1947601911423031"},{"key":"e_1_2_7_56_1","doi-asserted-by":"publisher","DOI":"10.1042\/BC20080221"},{"key":"e_1_2_7_57_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-nutr-072610-145138"},{"key":"e_1_2_7_58_1","doi-asserted-by":"publisher","DOI":"10.1111\/cei.12434"},{"key":"e_1_2_7_59_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cgh.2018.11.033"},{"key":"e_1_2_7_60_1","doi-asserted-by":"publisher","DOI":"10.1093\/ndt\/gft256"}],"container-title":["Alcoholism: Clinical and Experimental Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Facer.14331","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/acer.14331","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/acer.14331","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/acer.14331","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,8,3]],"date-time":"2024-08-03T12:25:28Z","timestamp":1722687928000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/acer.14331"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,4,21]]},"references-count":59,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2020,5]]}},"alternative-id":["10.1111\/acer.14331"],"URL":"https:\/\/doi.org\/10.1111\/acer.14331","archive":["Portico"],"relation":{},"ISSN":["0145-6008","1530-0277"],"issn-type":[{"value":"0145-6008","type":"print"},{"value":"1530-0277","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,4,21]]},"assertion":[{"value":"2019-11-14","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-03-19","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-04-21","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}