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Although benzodiazepines successfully mitigate such symptoms, this treatment does not significantly reduce recidivism rates in postdependent individuals. Instead, persistent affective disturbances that often emerge weeks to months after initial detoxification appear to play a significant role in relapse risk; however, it remains unclear whether genetic predispositions contribute to their emergence, severity, and\/or duration. Interestingly, significant genotypic and phenotypic differences have been observed among distinct C57BL\/6 (B6) substrains, and, in particular, C57BL\/6J (B6J) mice have been found to reliably exhibit higher voluntary ethanol (EtOH) intake and EtOH preference compared to several C57BL\/6N (B6N)\u2010derived substrains. To date, however, B6 substrains have not been directly compared on measures of acute withdrawal severity or affective\u2013behavioral disruption during extended abstinence.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Male and female B6J and B6NJ mice were exposed to either a 7\u2010day chronic intermittent EtOH vapor (CIE) protocol or to ordinary room air in inhalation chambers. Subsequently, blood EtOH concentrations and handling\u2010induced convulsions were evaluated during acute withdrawal, and mice were then tested weekly for affective behavior on the sucrose preference test, light\u2013dark box test, and forced swim test throughout 4\u00a0weeks of (forced) abstinence.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Despite documented differences in voluntary EtOH intake between these substrains, we found little evidence for substrain differences in either acute withdrawal or long\u2010term abstinence between B6J and B6NJ mice.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>In B6J and B6NJ mice, both the acute and long\u2010term sequelae of EtOH withdrawal are dependent on largely nonoverlapping gene networks relative to those underlying voluntary EtOH drinking.<\/jats:p><\/jats:sec>","DOI":"10.1111\/acer.14443","type":"journal-article","created":{"date-parts":[[2020,8,30]],"date-time":"2020-08-30T07:45:05Z","timestamp":1598773505000},"page":"2019-2030","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Affective Disruption During Forced Ethanol Abstinence in C57BL\/6J and C57BL\/6NJ Mice"],"prefix":"10.1111","volume":"44","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9676-5195","authenticated-orcid":false,"given":"Matthew C.","family":"Hartmann","sequence":"first","affiliation":[{"name":"From the Graduate School of Biomedical Science and Engineering (MCH, VK, AMR) University of Maine  Orono Maine"},{"name":"Department of Psychology (MCH, MMH, CGS, AMR) University of Maine  Orono Maine"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Megan M.","family":"Haney","sequence":"additional","affiliation":[{"name":"Department of Psychology (MCH, MMH, 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