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Pulse\u2010wave Doppler ultrasound measurements for umbilical arteries and ascending aorta were obtained post\u2010gavage (GD12, GD14, GD18) on 2 fetuses\/litter. RNA from the non\u2010decidual (labyrinthine and junctional zone) portion of placentas was isolated and processed for RNA\u2010seq and subsequent bioinformatic analyses, and the association between transcriptomic changes and fetal phenotypes assessed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Exposure to ethanol in pregnant mice on GD10 attenuates umbilical cord blood flow transiently during gestation, and is associated with indices of IUGR, specifically decreased fetal weight and morphometric indices of cranial growth. Moreover, RNA\u2010seq of the fetal portion of the placenta demonstrated that this single exposure has lasting transcriptomic changes, including upregulation of <jats:italic>Tet3<\/jats:italic>, which is associated with spontaneous abortion. Weighted gene co\u2010expression network analysis (WGCNA) identified erythrocyte differentiation and homeostasis as important pathways associated with improved umbilical cord blood flow as gestation progresses. WGCNA also identified sensory perception of chemical stimulus\/odorant and receptor activity as important pathways associated with cranial growth.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Our data suggest that PAE perturbs the expression of placental genes relevant for placental hematopoiesis and environmental sensing, resulting in transient impairment of umbilical cord blood flow and, subsequently, IUGR.<\/jats:p><\/jats:sec>","DOI":"10.1111\/acer.14846","type":"journal-article","created":{"date-parts":[[2022,4,27]],"date-time":"2022-04-27T06:34:53Z","timestamp":1651041293000},"page":"1036-1049","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Prenatal alcohol exposure contributes to negative pregnancy outcomes by altering fetal vascular dynamics and the placental transcriptome"],"prefix":"10.1111","volume":"46","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6477-9285","authenticated-orcid":false,"given":"Marisa R.","family":"Pinson","sequence":"first","affiliation":[{"name":"Department of Neuroscience and Experimental Therapeutics Texas A&amp;M College of Medicine  Bryan Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander M.","family":"Tseng","sequence":"additional","affiliation":[{"name":"Department of Neuroscience and Experimental Therapeutics Texas A&amp;M College of Medicine  Bryan Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amy","family":"Adams","sequence":"additional","affiliation":[{"name":"Department of Neuroscience and Experimental Therapeutics Texas A&amp;M College of Medicine  Bryan Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tenley E.","family":"Lehman","sequence":"additional","affiliation":[{"name":"Department of Neuroscience and Experimental Therapeutics Texas A&amp;M College of Medicine  Bryan Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karen","family":"Chung","sequence":"additional","affiliation":[{"name":"Department of Neuroscience and Experimental Therapeutics Texas A&amp;M College of Medicine  Bryan Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jessica","family":"Gutierrez","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering University of Houston  Houston Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kirill V.","family":"Larin","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering University of Houston  Houston Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4675-7722","authenticated-orcid":false,"given":"Christina","family":"Chambers","sequence":"additional","affiliation":[{"name":"Clinical and Translational Research Institute University of California San Diego  San Diego California USA"},{"name":"Department of Pediatrics University of California San Diego  San Diego California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8359-892X","authenticated-orcid":false,"given":"Rajesh C.","family":"Miranda","sequence":"additional","affiliation":[{"name":"Department of Neuroscience and Experimental Therapeutics Texas A&amp;M College of Medicine  Bryan Texas USA"},{"name":"Women\u2019s Health in Neuroscience Program Texas A&amp;M University College of Medicine  Bryan Texas USA"},{"name":"Interdisciplinary Program of Genetics Texas A&amp;M University  College Station Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"name":"Collaborative Initiative on Fetal Alcohol Spectrum 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