{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,27]],"date-time":"2026-03-27T17:19:36Z","timestamp":1774631976788,"version":"3.50.1"},"reference-count":63,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2022,8,2]],"date-time":"2022-08-02T00:00:00Z","timestamp":1659398400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000009","name":"Foundation for the National Institutes of Health","doi-asserted-by":"publisher","award":["R01AG067758"],"award-info":[{"award-number":["R01AG067758"]}],"id":[{"id":"10.13039\/100000009","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Alcoholism Clin &amp;amp; Exp Res"],"published-print":{"date-parts":[[2022,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Weakness is a common clinical symptom reported in individuals with chronic alcohol use disorder. However, it remains unclear whether low strength in these individuals is directly related to excessive ethanol intake, other deleterious factors (lifestyle, environment, genetics, etc.), or a combination of both. Therefore, we examined whether (and how) ethanol reduces the muscle\u2019s force\u2010producing capacity using a controlled in vivo preclinical mouse model of excessive ethanol intake.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>To establish whether chronic ethanol consumption causes weakness, C57BL\/6 female mice consumed 20% ethanol for 40\u2009weeks (following a 2\u2010week ethanol ramping period), and various measures of muscular force were quantified. Functional measures included all\u2010limb grip strength and in vivo contractility of the left ankle dorsiflexors and plantarflexors. Once confirmed that mice consuming ethanol were weaker than age\u2010matched controls, we sought to determine the potential neuromuscular mechanisms of muscle dysfunction by assessing neuromuscular excitation, muscle quantity, and muscle quality.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Mice consuming chronic ethanol were 13 to 16% weaker (<jats:italic>p<\/jats:italic>\u00a0\u2264\u20090.016) than controls (i.e., mice consuming 100% water) with the negative impact of ethanol on voluntary grip strength\u00a0(<jats:italic>\u019e<\/jats:italic><jats:sup>2<\/jats:sup>\u00a0=\u20090.603) being slightly larger than that of electrically stimulated muscle contractility (<jats:italic>\u019e<\/jats:italic><jats:sup>2<\/jats:sup>\u00a0=\u20090.482). Relative to controls, lean mass and muscle wet masses were 9 to 16% lower in ethanol\u2010consuming mice (<jats:italic>p<\/jats:italic>\u00a0\u2264\u20090.048, <jats:italic>\u019e<\/jats:italic><jats:sup>2<\/jats:sup>\u00a0\u2265\u20090.268). No significant changes were observed between groups for indices of neuromuscular excitation at the level of the motor unit, neuromuscular junction, or plasmalemma (<jats:italic>p<\/jats:italic>\u00a0\u2265\u20090.259, <jats:italic>\u019e<\/jats:italic><jats:sup>2<\/jats:sup>\u00a0\u2264\u20090.097), nor was muscle quality altered after 40\u2009weeks of 20% ethanol consumption (<jats:italic>p<\/jats:italic>\u00a0\u2265\u20090.695, <jats:italic>\u019e<\/jats:italic><jats:sup>2<\/jats:sup>\u00a0\u2264\u20090.012).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Together, these findings establish that chronic ethanol consumption in mice induces a substantial weakness in vivo that we interpret to be primarily due to muscle atrophy (i.e., reduced muscle quantity) and possibly, to a lesser degree, loss of central neural drive.<\/jats:p><\/jats:sec>","DOI":"10.1111\/acer.14907","type":"journal-article","created":{"date-parts":[[2022,7,23]],"date-time":"2022-07-23T09:09:36Z","timestamp":1658567376000},"page":"1636-1647","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Neuromuscular mechanisms of weakness in a mouse model of chronic alcoholic myopathy"],"prefix":"10.1111","volume":"46","author":[{"given":"Samantha E.","family":"Moser","sequence":"first","affiliation":[{"name":"Honors Tutorial College Ohio University  Athens Ohio USA"},{"name":"Ohio Musculoskeletal and Neurological Institute (OMNI) Ohio University  Athens Ohio USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Austin M.","family":"Brown","sequence":"additional","affiliation":[{"name":"Honors Tutorial College Ohio University  Athens Ohio USA"},{"name":"Ohio Musculoskeletal and Neurological Institute (OMNI) Ohio University  Athens Ohio USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brian C.","family":"Clark","sequence":"additional","affiliation":[{"name":"Ohio Musculoskeletal and Neurological Institute (OMNI) Ohio University  Athens Ohio USA"},{"name":"Department of Biomedical Sciences Ohio University  Athens Ohio USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W. David","family":"Arnold","sequence":"additional","affiliation":[{"name":"Department of Neurology The Ohio State University Wexner Medical Center  Columbus Ohio USA"},{"name":"Department of Physical Medicine and Rehabilitation The Ohio State University Wexner Medical Center  Columbus Ohio USA"},{"name":"Department of Neuroscience The Ohio State University Wexner Medical Center  Columbus Ohio USA"},{"name":"Department of Physiology and Cell Biology The Ohio State University Wexner Medical Center  Columbus Ohio USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2457-1911","authenticated-orcid":false,"given":"Cory W.","family":"Baumann","sequence":"additional","affiliation":[{"name":"Ohio Musculoskeletal and Neurological Institute (OMNI) Ohio University  Athens Ohio USA"},{"name":"Department of Biomedical Sciences Ohio University  Athens Ohio USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,8,2]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0955-2863(03)00114-1"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-510X(98)00266-4"},{"key":"e_1_2_8_4_1","first-page":"1","article-title":"Electrophysiological motor unit number estimation (MUNE) measuring compound muscle action potential (CMAP) in mouse hindlimb muscles","volume":"103","author":"Arnold W.D.","year":"2015","journal-title":"Journal of Visualized Experiments"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.00321.2016"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.18632\/aging.101692"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.14814\/phy2.12081"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01945792"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exger.2018.11.003"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/gerona\/63.8.829"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnmol.2016.00008"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/acer.14179"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/acer.13159"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1186\/2050-490X-1-2"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1530-0277.2010.01234.x"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/0741-8329(87)90091-7"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1530-0277.1998.tb05911.x"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1136\/jnnp.34.1.86"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1530-0277.2007.00530.x"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuropharm.2017.04.007"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.15288\/jsa.1987.48.212"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/133.4.1154"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.01058.2003"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1042\/cs0630161"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00415-018-9123-1"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00689764"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M705503200"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-nutr-071816-064642"},{"key":"e_1_2_8_29_1","first-page":"640","article-title":"Identifying characteristics of frailty in female mice using a phenotype assessment tool","volume":"74","author":"Kwak D.","year":"2019","journal-title":"The Journals of Gerontology. 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