{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T07:01:53Z","timestamp":1792479713657,"version":"4.5.0"},"reference-count":50,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2022,9,23]],"date-time":"2022-09-23T00:00:00Z","timestamp":1663891200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000050","name":"National Heart, Lung, and Blood Institute","doi-asserted-by":"publisher","award":["T32HL007736"],"award-info":[{"award-number":["T32HL007736"]}],"id":[{"id":"10.13039\/100000050","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000027","name":"National Institute on Alcohol Abuse and Alcoholism","doi-asserted-by":"publisher","award":["F31AA029597"],"award-info":[{"award-number":["F31AA029597"]}],"id":[{"id":"10.13039\/100000027","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000027","name":"National Institute on Alcohol Abuse and Alcoholism","doi-asserted-by":"publisher","award":["P50AA0222534"],"award-info":[{"award-number":["P50AA0222534"]}],"id":[{"id":"10.13039\/100000027","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000027","name":"National Institute on Alcohol Abuse and Alcoholism","doi-asserted-by":"publisher","award":["R21AA026371"],"award-info":[{"award-number":["R21AA026371"]}],"id":[{"id":"10.13039\/100000027","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Alcoholism Clin &amp;amp; Exp Res"],"published-print":{"date-parts":[[2022,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Fetal alcohol spectrum disorders (FASD) occur in children who were exposed to alcohol in utero and are manifested in a wide range of neurocognitive deficits. These deficits could be caused by alterations to the cortical microvasculature that are controlled by post\u2010transcriptional regulators such as microRNAs.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Using an established mouse model of moderate prenatal alcohol exposure (PAE), we isolated cortices (CTX) and brain microvascular endothelial cells (BMVECs) at embryonic day 18 (E18) and examined the expression of miR\u2010150\u20105p and potential downstream targets. Cellular transfections and intrauterine injections with LNA\u2122 mimics or inhibitors were used to test miR\u2010150\u20105p regulation of novel target vascular endothelial zinc finger 1 (Vezf1). Dual\u2010luciferase assays were used to assess the direct binding of miR\u2010150\u20105p to the Vezf1 3\u2032UTR. The effects of miR\u2010150\u20105p and Vezf1 on endothelial cell function were determined by in vitro migration and tube formation assays.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We found that miR\u2010150\u20105p was upregulated and Vezf1 was downregulated during PAE in the E18 CTX and BMVECs. Transfection with miR\u2010150\u20105p mimics resulted in decreased Vezf1 expression in BMVECs, while miR\u2010150\u20105p inhibition did the opposite. Dual\u2010luciferase assays revealed direct binding of miR\u2010150\u20105p with the Vezf1 3\u2032UTR. Intrauterine injections showed that miR\u2010150\u20105p regulates the expression of Vezf1 in vivo during PAE. miR\u2010150\u20105p overexpression decreased BMVEC migration and tube formation, while miR\u2010150\u20105p inhibition enhanced migration and tube formation. Vezf1 overexpression rescued the effects of the miR\u2010150\u20105p mimic. Alcohol treatment of BMVECs increased miR\u2010150\u20105p expression and inhibited migration and tube formation. Finally, miR\u2010150\u20105p inhibition and Vezf1 overexpression rescued the negative effects of alcohol on migration and tube formation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>miR\u2010150\u20105p regulation of Vezf1 results in altered endothelial cell function during alcohol exposure. Further, miR\u2010150\u20105p inhibition of Vezf1 may adversely alter the development of the cortical microvasculature during PAE and contribute to deficits seen in patients with FASD.<\/jats:p><\/jats:sec>","DOI":"10.1111\/acer.14939","type":"journal-article","created":{"date-parts":[[2022,9,16]],"date-time":"2022-09-16T01:30:01Z","timestamp":1663291801000},"page":"1953-1966","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["<scp>MicroRNA<\/scp>\u2010150\u20105p is upregulated in the brain microvasculature during prenatal alcohol exposure and inhibits the angiogenic factor Vezf1"],"prefix":"10.1111","volume":"46","author":[{"given":"Gabriela","family":"Perales","sequence":"first","affiliation":[{"name":"Department of Cell Biology and Physiology University of New Mexico Health Sciences Center  Albuquerque New Mexico USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marissa","family":"Westenskow","sequence":"additional","affiliation":[{"name":"Department of Cell Biology and Physiology University of New Mexico Health Sciences Center  Albuquerque New Mexico USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roxana","family":"Gutierrez","sequence":"additional","affiliation":[{"name":"Department of Cell Biology and Physiology University of New Mexico Health Sciences Center  Albuquerque New Mexico USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kevin K.","family":"Caldwell","sequence":"additional","affiliation":[{"name":"Department of Neurosciences University of New Mexico Health Sciences Center  Albuquerque New Mexico USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea M.","family":"Allan","sequence":"additional","affiliation":[{"name":"Department of Neurosciences University of New Mexico Health Sciences Center  Albuquerque New Mexico USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8179-4919","authenticated-orcid":false,"given":"Amy S.","family":"Gardiner","sequence":"additional","affiliation":[{"name":"Department of Cell Biology and Physiology University of New Mexico Health Sciences Center  Albuquerque New Mexico USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,9,23]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M105166200"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.RA118.002911"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1530-0277.2011.01676.x"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0165081"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(04)00045-5"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/pch\/14.4.231"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1530-0277.2011.01644.x"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-79350-7"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1177\/0271678X17728163"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/bdrc.21129"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1542\/peds.2014-2171"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1080\/15476286.2015.1048954"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.15.1168"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0705458"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.201500126"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2008.07.008"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1101\/gr.082701.108"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/acer.13026"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms3824"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1203\/00006450-199206000-00021"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1203\/00006450-199707000-00007"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature04481"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/cns.12525"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a008144"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1186\/1742-2094-11-131"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1093\/cercor\/bhu066"},{"issue":"105","key":"e_1_2_7_28_1","first-page":"53196","article-title":"Modeling encephalopathy of prematurity using prenatal hypoxia\u2010ischemia with intra\u2010amniotic lipopolysaccharide in rats","author":"Jantzie L.L.","year":"2015","journal-title":"Journal of Visualized Experiments"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1002\/ana.23699"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0103965"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2005.04.003"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40478-017-0444-6"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0115577"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1508426112"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.7150\/ijbs.27029"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.placenta.2015.10.009"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2017.21896"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1080\/10623320500227101"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00080.2005"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9378(87)80250-8"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.1269-07.2007"},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnins.2020.00519"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.1038\/mt.2008.104"},{"key":"e_1_2_7_45_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12035-020-02214-8"},{"key":"e_1_2_7_46_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-59745-242-7_9"},{"key":"e_1_2_7_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gde.2009.04.002"},{"key":"e_1_2_7_48_1","doi-asserted-by":"publisher","DOI":"10.3892\/mmr.2020.11672"},{"key":"e_1_2_7_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2007.07.021"},{"key":"e_1_2_7_50_1","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.1998.9144"},{"key":"e_1_2_7_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2010.06.010"}],"container-title":["Alcoholism: Clinical and Experimental Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/acer.14939","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/acer.14939","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/acer.14939","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,22]],"date-time":"2023-08-22T06:26:46Z","timestamp":1692685606000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/acer.14939"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,9,23]]},"references-count":50,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2022,11]]}},"alternative-id":["10.1111\/acer.14939"],"URL":"https:\/\/doi.org\/10.1111\/acer.14939","archive":["Portico"],"relation":{},"ISSN":["0145-6008","1530-0277"],"issn-type":[{"value":"0145-6008","type":"print"},{"value":"1530-0277","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,9,23]]},"assertion":[{"value":"2022-03-11","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-09-06","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-09-23","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}