{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T05:16:12Z","timestamp":1771478172836,"version":"3.50.1"},"reference-count":73,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2022,12,1]],"date-time":"2022-12-01T00:00:00Z","timestamp":1669852800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000066","name":"National Institute of Environmental Health Sciences","doi-asserted-by":"publisher","award":["K01ES033289"],"award-info":[{"award-number":["K01ES033289"]}],"id":[{"id":"10.13039\/100000066","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000066","name":"National Institute of Environmental Health Sciences","doi-asserted-by":"publisher","award":["P30ES030283"],"award-info":[{"award-number":["P30ES030283"]}],"id":[{"id":"10.13039\/100000066","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000066","name":"National Institute of Environmental Health Sciences","doi-asserted-by":"publisher","award":["P42ES023716"],"award-info":[{"award-number":["P42ES023716"]}],"id":[{"id":"10.13039\/100000066","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000066","name":"National Institute of Environmental Health Sciences","doi-asserted-by":"publisher","award":["R01AA024102"],"award-info":[{"award-number":["R01AA024102"]}],"id":[{"id":"10.13039\/100000066","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000066","name":"National Institute of Environmental Health Sciences","doi-asserted-by":"publisher","award":["R01ES032189"],"award-info":[{"award-number":["R01ES032189"]}],"id":[{"id":"10.13039\/100000066","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000066","name":"National Institute of Environmental Health Sciences","doi-asserted-by":"publisher","award":["R21ES031510"],"award-info":[{"award-number":["R21ES031510"]}],"id":[{"id":"10.13039\/100000066","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000066","name":"National Institute of Environmental Health Sciences","doi-asserted-by":"publisher","award":["R35ES028373"],"award-info":[{"award-number":["R35ES028373"]}],"id":[{"id":"10.13039\/100000066","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000066","name":"National Institute of Environmental Health Sciences","doi-asserted-by":"publisher","award":["T32ES011564"],"award-info":[{"award-number":["T32ES011564"]}],"id":[{"id":"10.13039\/100000066","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000066","name":"National Institute of Environmental Health Sciences","doi-asserted-by":"publisher","award":["T35ES014559"],"award-info":[{"award-number":["T35ES014559"]}],"id":[{"id":"10.13039\/100000066","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["P20GM113226"],"award-info":[{"award-number":["P20GM113226"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000027","name":"National Institute on Alcohol Abuse and Alcoholism","doi-asserted-by":"publisher","award":["P50AA024337"],"award-info":[{"award-number":["P50AA024337"]}],"id":[{"id":"10.13039\/100000027","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Alcohol: Clinical and Experimental Research"],"published-print":{"date-parts":[[2023,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>The prevalence of alcohol\u2010associated liver disease (ALD), a subtype of fatty liver disease (FLD), continues to rise. ALD is a major cause of preventable death. Polychlorinated biphenyl (PCB) 126 is an environmentally relevant, dioxin\u2010like pollutant whose negative metabolic effects have been well documented. In human and animal studies,\u00a0PCB\u00a0has been associated with\u00a0the severity of nonalcoholic fatty liver disease (NAFLD). However, few studies have investigated whether exposures to environmental toxicants can worsen ALD. Thus, the objective of the current study was to develop an alcohol\u2010plus\u2010toxicant model to study how an environmental pollutant, PCB 126, impacts rodent ALD pathology.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Briefly, male C57BL\/6J mice were exposed to 0.2\u00a0mg\/kg PCB 126 or corn oil vehicle four days prior to ethanol feeding using the chronic\u2010binge (10\u2010plus\u2010one) model.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Concentrations of macromolecules, including hepatic lipids, carbohydrates, and protein (albumin) were impacted. Exposure to PCB 126 exacerbated hepatic steatosis and hepatomegaly in mice exposed to the chemical and fed an ethanol diet. Gene expression and the analysis of blood chemistry showed a potential net increase and retention of hepatic lipids and reductions in lipid oxidation and clearance capabilities. Depletion of glycogen and glucose was evident, which contributes to disease progression by generating systemic malnutrition. Granulocytic immune infiltrates were present but driven solely by ethanol feeding. Hepatic albumin gene expression and plasma levels were decreased by ~50% indicating a potential compromise of liver function. Finally, gene expression analyses indicated that the aryl hydrocarbon receptor and constitutive androstane receptor were activated by PCB 126 and ethanol, respectively.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Various environmental toxicants are known to modify or enhance FLD in high\u2010fat diet models. Findings from the present study suggest that they interact with other lifestyle factors such as alcohol consumption to reprogram intermediary metabolism resulting in exacerbated ethanol\u2010associated systemic malnutrition in ALD.<\/jats:p><\/jats:sec>","DOI":"10.1111\/acer.14976","type":"journal-article","created":{"date-parts":[[2022,11,15]],"date-time":"2022-11-15T06:51:23Z","timestamp":1668495083000},"page":"60-75","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["The environmental pollutant, polychlorinated biphenyl 126, alters liver function in a rodent model of alcohol\u2010associated liver disease"],"prefix":"10.1111","volume":"47","author":[{"given":"Tyler C.","family":"Gripshover","sequence":"first","affiliation":[{"name":"Department of Pharmacology and Toxicology University of Louisville School of Medicine  Louisville Kentucky USA"},{"name":"Division of Gastroenterology, Hepatology and Nutrition, Department of Medicine, School of Medicine University of Louisville  Louisville Kentucky USA"},{"name":"University of Louisville Superfund Research Program University of Louisville  Louisville Kentucky USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Banrida","family":"Wahlang","sequence":"additional","affiliation":[{"name":"Division of Gastroenterology, Hepatology and Nutrition, Department of Medicine, School of Medicine University of Louisville  Louisville Kentucky USA"},{"name":"University of Louisville Superfund Research Program University of Louisville  Louisville Kentucky USA"},{"name":"Hepatobiology and Toxicology COBRE University of Louisville School of Medicine  Louisville Kentucky USA"},{"name":"University of Louisville Alcohol Research Center University of Louisville  Louisville Kentucky USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kimberly Z.","family":"Head","sequence":"additional","affiliation":[{"name":"Division of Gastroenterology, Hepatology and Nutrition, Department of Medicine, School of Medicine University of Louisville  Louisville Kentucky USA"},{"name":"Hepatobiology and Toxicology COBRE University of Louisville School of Medicine  Louisville Kentucky USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jamie L.","family":"Young","sequence":"additional","affiliation":[{"name":"Division of Gastroenterology, Hepatology and Nutrition, Department of Medicine, School of Medicine University of Louisville  Louisville Kentucky USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jianzhu","family":"Luo","sequence":"additional","affiliation":[{"name":"Division of Gastroenterology, Hepatology and Nutrition, Department of Medicine, School of Medicine University of Louisville  Louisville Kentucky USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Muhammad T.","family":"Mustafa","sequence":"additional","affiliation":[{"name":"The Center for Integrative Environmental Health Sciences University of Louisville  Louisville Kentucky USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Irina A.","family":"Kirpich","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Toxicology University of Louisville School of Medicine  Louisville Kentucky USA"},{"name":"Division of Gastroenterology, Hepatology and Nutrition, Department of Medicine, School of Medicine University of Louisville  Louisville Kentucky USA"},{"name":"Hepatobiology and Toxicology COBRE University of Louisville School of Medicine  Louisville Kentucky USA"},{"name":"University of Louisville Alcohol Research Center University of Louisville  Louisville Kentucky USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2560-6696","authenticated-orcid":false,"given":"Matthew C.","family":"Cave","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Toxicology University of Louisville School of Medicine  Louisville Kentucky USA"},{"name":"Division of Gastroenterology, Hepatology and Nutrition, Department of Medicine, School of Medicine University of Louisville  Louisville Kentucky USA"},{"name":"University of Louisville Superfund Research Program University of Louisville  Louisville Kentucky USA"},{"name":"Hepatobiology and Toxicology COBRE University of Louisville School of Medicine  Louisville Kentucky USA"},{"name":"University of Louisville Alcohol Research Center University of Louisville  Louisville Kentucky USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,12]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dsx.2018.03.016"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1177\/0192623314549960"},{"key":"e_1_2_9_4_1","first-page":"305","volume-title":"Toxicological profile for polychlorinated biphenyls (PCBs)","author":"ATSDR","year":"2000"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cld.2018.09.011"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2009.01.003"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1515\/bc.2010.137"},{"key":"e_1_2_9_8_1","first-page":"335","article-title":"Increased hepatocellular uptake of long chain fatty acids occurs by different mechanisms in fatty livers due to obesity or excess ethanol use, contributing to development of steatohepatitis in both settings","volume":"116","author":"Berk P.D.","year":"2005","journal-title":"Transactions of the American Clinical and Climatological Association"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2013.032"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1139\/o59-099"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.alcohol.2012.08.004"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1289\/EHP9467"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1093\/toxsci\/kfy076"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.30866"},{"issue":"2","key":"e_1_2_9_15_1","first-page":"38","article-title":"The Anniston community health survey","volume":"83","author":"Cusack C.","year":"2020","journal-title":"Journal of Environmental Health"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chemosphere.2018.10.196"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.envint.2020.105481"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v13.i37.4974"},{"key":"e_1_2_9_19_1","unstructured":"EPA. (2022)What are PCB's?. Available from:https:\/\/www3.epa.gov\/region1\/eco\/uep\/pcb.html[Accessed 24th April 2022]"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1093\/toxsci\/kfv215"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.2174\/1389201015666141122203509"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms22010049"},{"issue":"2","key":"e_1_2_9_23_1","first-page":"64","article-title":"Comorbidities and nonalcoholic fatty liver disease: the chicken, the egg, or both?","volume":"36","author":"Glass L.M.","year":"2019","journal-title":"Federal Practitioner: For the Health Care Professionals of the VA, DoD, and PHS"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1258\/ebm.2009.009249"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1093\/toxsci\/kfy004"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-pharmtox-010818-021304"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.reprotox.2016.10.001"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1006\/taap.2000.9026"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.00479-16"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00044-020-02581-w"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.apsb.2021.10.014"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cld.2018.09.009"},{"issue":"1","key":"e_1_2_9_33_1","article-title":"Combined exposure to polychlorinated biphenyls and high\u2010fat diet modifies the global epitranscriptomic landscape in mouse liver","volume":"7","author":"Klinge C.M.","year":"2021","journal-title":"Environmental Epigenetics"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.3389\/fendo.2020.00556"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.alcohol.2004.07.008"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrgastro.2015.35"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.alcohol.2013.04.004"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1057\/s41271-018-0146-8"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1530-0277.2010.01405.x"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00204-018-2235-7"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.1840400428"},{"issue":"3","key":"e_1_2_9_42_1","first-page":"167","article-title":"Pathogenesis and prevention of hepatic steatosis","volume":"11","author":"Nassir F.","year":"2015","journal-title":"Gastroenterology & Hepatology"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0184842"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12876-020-01270-7"},{"key":"e_1_2_9_45_1","first-page":"4","article-title":"NTP toxicology and carcinogenesis studies of 3,3\u2032,4,4\u2032,5\u2010pentachlorobiphenyl (PCB 126) (CAS No. 57465\u201328\u20108) in female Harlan Sprague\u2010Dawley rats (Gavage Studies)","volume":"520","author":"NTP","year":"2006","journal-title":"National Toxicology Program Technical Report Series"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1093\/toxsci\/kfx275"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jnutbio.2016.03.016"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21218351"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0230629"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijerph16183415"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21186654"},{"key":"e_1_2_9_52_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v20.i43.15955"},{"key":"e_1_2_9_53_1","doi-asserted-by":"publisher","DOI":"10.2147\/DMSO.S264851"},{"key":"e_1_2_9_54_1","doi-asserted-by":"publisher","DOI":"10.1080\/00498254.2019.1566582"},{"key":"e_1_2_9_55_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.taap.2018.10.011"},{"key":"e_1_2_9_56_1","doi-asserted-by":"publisher","DOI":"10.1262\/jrd.18062"},{"key":"e_1_2_9_57_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.physbeh.2017.03.024"},{"key":"e_1_2_9_58_1","doi-asserted-by":"publisher","DOI":"10.3390\/biom5042223"},{"key":"e_1_2_9_59_1","doi-asserted-by":"publisher","DOI":"10.1080\/07853890.2019.1693056"},{"key":"e_1_2_9_60_1","doi-asserted-by":"publisher","DOI":"10.1093\/toxsci\/kfs204"},{"key":"e_1_2_9_61_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0741-8329(99)00030-0"},{"key":"e_1_2_9_62_1","doi-asserted-by":"publisher","DOI":"10.1177\/0192623312468517"},{"key":"e_1_2_9_63_1","doi-asserted-by":"publisher","DOI":"10.1093\/toxsci\/kfu083"},{"key":"e_1_2_9_64_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40572-019-00232-w"},{"key":"e_1_2_9_65_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tox.2017.02.001"},{"key":"e_1_2_9_66_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.taap.2014.06.019"},{"key":"e_1_2_9_67_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.taap.2019.02.006"},{"key":"e_1_2_9_68_1","volume-title":"Global status report on alcohol and health 2018","author":"WHO","year":"2018"},{"key":"e_1_2_9_69_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.aax4043"},{"key":"e_1_2_9_70_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2018.10.037"},{"key":"e_1_2_9_71_1","doi-asserted-by":"publisher","DOI":"10.1093\/alcalc\/agl017"},{"key":"e_1_2_9_72_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrgastro.2017.109"},{"key":"e_1_2_9_73_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1530-0277.2012.01787.x"},{"key":"e_1_2_9_74_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0037103"}],"container-title":["Alcohol: Clinical and Experimental Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/acer.14976","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/acer.14976","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/acer.14976","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,4]],"date-time":"2024-01-04T00:18:54Z","timestamp":1704327534000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/acer.14976"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,12]]},"references-count":73,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2023,1]]}},"alternative-id":["10.1111\/acer.14976"],"URL":"https:\/\/doi.org\/10.1111\/acer.14976","archive":["Portico"],"relation":{},"ISSN":["2993-7175","2993-7175"],"issn-type":[{"value":"2993-7175","type":"print"},{"value":"2993-7175","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,12]]},"assertion":[{"value":"2022-06-03","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-11-10","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-12-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}