{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T15:25:25Z","timestamp":1791559525023,"version":"4.3.4"},"reference-count":46,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2023,2,17]],"date-time":"2023-02-17T00:00:00Z","timestamp":1676592000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100010010","name":"Shanxi Province Science Foundation for Youths","doi-asserted-by":"publisher","award":["2021 (20210302124417)"],"award-info":[{"award-number":["2021 (20210302124417)"]}],"id":[{"id":"10.13039\/501100010010","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Alcohol: Clinical and Experimental Research"],"published-print":{"date-parts":[[2023,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Alcohol\u2010associated hepatic fibrosis is a widespread liver disease with no effective treatment. Recent studies have indicated that interleukin\u201022 (IL\u201022) can ameliorate alcohol\u2010associated liver disease. However, the mechanism underlying the role of IL\u201022 in alcohol\u2010associated hepatic fibrosis remains unclear. Therefore, we investigated the effect of IL\u201022 in a mouse model of alcohol\u2010associated hepatic fibrosis and its underlying mechanisms.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Alcohol\u2010associated hepatic fibrosis was induced by feeding male C57BL\/6J mice with a Lieber\u2010DeCarli liquid diet containing 4% ethyl alcohol for 8\u2009weeks and injecting them with 5% tetrachloromethane (CCl<jats:sub>4<\/jats:sub>) intraperitoneally for the last 4\u2009weeks. During the last 4\u2009weeks, IL\u201022 was also administered. We investigated the role of IL\u201022 in autophagy and the PI3K\/AKT\/mTOR signaling pathway using a 3\u2010methyladenine intraperitoneal injection in the mice treated with IL\u201022. The effects of IL\u201022 on alcohol\u2010associated hepatic fibrosis, autophagy\u2010related gene expression, and PI3K\/AKT\/mTOR activity were assessed using histopathology, biochemical analysis, transmission electron microscopy, quantitative real\u2010time PCR, immunohistochemistry, and western blotting.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Mice treated with ethanol and CCl<jats:sub>4<\/jats:sub> displayed distinct liver injuries, including hepatocyte necrosis, inflammatory cell infiltration, and hepatic fibrosis, which were substantially attenuated by IL\u201022 treatment. In addition, we found that IL\u201022 regulated the expression of autophagy\u2010related genes and inhibited the PI3K\/AKT\/mTOR pathway, as evidenced by the reduction in p\u2010PI3K, p\u2010AKT, and p\u2010mTOR expression after IL\u201022 treatment.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>IL\u201022 exerts a marked protective effect against alcohol\u2010associated hepatic fibrosis. Its effect may be partly related to the alteration of autophagy\u2010related gene expression and inhibition of the PI3K\/AKT\/mTOR pathway in the liver.<\/jats:p><\/jats:sec>","DOI":"10.1111\/acer.15021","type":"journal-article","created":{"date-parts":[[2023,2,17]],"date-time":"2023-02-17T08:47:06Z","timestamp":1676623626000},"page":"448-458","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Interleukin\u201022 alleviates alcohol\u2010associated hepatic fibrosis, inhibits autophagy, and suppresses the <scp>PI3K<\/scp>\/<scp>AKT<\/scp>\/<scp>mTOR<\/scp> pathway in mice"],"prefix":"10.1111","volume":"47","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2247-8446","authenticated-orcid":false,"given":"Yu\u2010Xi","family":"Meng","sequence":"first","affiliation":[{"name":"Shanxi Medical University  Taiyuan China"},{"name":"Department of Gastroenterology The Second Hospital of Shanxi Medical University  Taiyuan 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