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Many neuropeptide systems are thought to become dysregulated in AUD; however, whether repeated cycles of binge\u2010like ethanol consumption and abstinence following binge\u2010like drinking alter neuropeptide mRNA in key brain regions, such as the medial prefrontal cortex (mPFC), insular cortex (IC), amygdala, and lateral hypothalamus (LH), remains unknown.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Male and female mice underwent 0, 3, or 6 cycles of binge\u2010like ethanol consumption using the \u201cDrinking in the Dark\u201d (DID) paradigm. Brain tissue was collected either immediately following the final session of DID or after a 24\u2010h period of abstinence, and quantitative polymerase chain reaction (qPCR) was performed to assess how repeated cycles of binge\u2010like ethanol intake and abstinence alter relative mRNA expression for 22 neuropeptide\u2010related targets.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We observed that repeated cycles of binge\u2010like ethanol consumption and abstinence altered relative mRNA expression for 11 targets in the mPFC, five targets in the IC, eight targets in the amygdala, and two targets in the LH. Two of these alterations were specific to female mice, while one was specific to male mice.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>These data suggest that neuropeptide mRNA is altered by repeated cycles of binge\u2010like ethanol intake and abstinence in a brain region and sex\u2010dependent manner. The current findings provide a useful foundation from which to explore potential targets to decrease binge\u2010like ethanol consumption and prevent the development of AUD.<\/jats:p><\/jats:sec>","DOI":"10.1111\/acer.15536","type":"journal-article","created":{"date-parts":[[2025,1,31]],"date-time":"2025-01-31T10:59:51Z","timestamp":1738321191000},"page":"573-586","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Repeated cycles of binge\u2010like ethanol consumption and abstinence alter neuropeptide m<scp>RNA<\/scp> in prefrontal and insular cortex, amygdala, and lateral hypothalamus of male and female <scp>C<\/scp>57<scp>BL<\/scp>\/6<scp>J<\/scp> mice"],"prefix":"10.1111","volume":"49","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3046-504X","authenticated-orcid":false,"given":"Anne M.","family":"Dankert","sequence":"first","affiliation":[{"name":"Department of Psychology &amp; Neuroscience University of North Carolina at Chapel Hill  Chapel Hill North Carolina USA"},{"name":"The Bowles Center for Alcohol Studies University of North Carolina at Chapel Hill  Chapel Hill North Carolina USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4747-4495","authenticated-orcid":false,"given":"Thomas L.","family":"Kash","sequence":"additional","affiliation":[{"name":"The Bowles Center for Alcohol Studies University of North Carolina at Chapel Hill  Chapel Hill North Carolina USA"},{"name":"Department of Pharmacology University of North Carolina at Chapel Hill  Chapel Hill North Carolina USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8908-9256","authenticated-orcid":false,"given":"Todd E.","family":"Thiele","sequence":"additional","affiliation":[{"name":"Department of Psychology &amp; 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