{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T09:43:46Z","timestamp":1785836626776,"version":"3.56.0"},"reference-count":64,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2026,7,10]],"date-time":"2026-07-10T00:00:00Z","timestamp":1783641600000},"content-version":"vor","delay-in-days":9,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2019\/01686\u20100"],"award-info":[{"award-number":["2019\/01686\u20100"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","award":["88887.695072\/2022\u201000"],"award-info":[{"award-number":["88887.695072\/2022\u201000"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Alcohol, Clinical and Experimental Research"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Decision\u2010making relies on risk assessment as a critical process that enables organisms to evaluate threats before exploring potentially dangerous areas. While adolescence is a period of heightened vulnerability to external factors such as substance use and stress, it remains unclear how these factors disrupt risk assessment, particularly at the level of behavior structure: sequential actions, organization and transition probabilities between defensive and exploratory behaviors. We investigated whether repeated ethanol intoxication or pharmacological stress during late adolescence alters behavioral structure in early adulthood.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Male and female C57BL\/6 mice received four intermittent injections of saline, ethanol (4.0\u2009g\/kg), or the \u03b12\u2010adrenergic antagonist yohimbine (2.0\u2009mg\/kg) during late adolescence (7\u20138\n                      <jats:sup>th<\/jats:sup>\n                      \u2009weeks). In early adulthood (9\n                      <jats:sup>th<\/jats:sup>\n                      week), animals were tested in the Light\u2013Dark Box and Elevated Plus Maze. In addition to measuring classical anxiety\u2010like and exploratory behavior, we focused on risk assessment behaviors (stretches\/head\u2010outs) categorized as NoGo (evaluation without entering the aversive area) or Go (evaluation followed by entry). We used 1\n                      <jats:sup>st<\/jats:sup>\n                      order transition probability\u00a0Markov chain analyses to map the transitions between these states, defining the behavioral structure.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Both ethanol and yohimbine produced persistent reductions in NoGo risk assessment behaviors in adulthood, in both sexes, with minimal effects on classical anxiety\u2010like and exploratory measures. Transition probability and correlation analyses revealed a reorganization of behavioral structure, characterized by reduced transitions toward risk assessment events and a bias toward risk\u2010taking actions.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>These findings indicate that repeated late adolescent alcohol intoxication or stress exposure induces long\u2010lasting alterations in risk assessment and decision\u2010making strategies.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/acer.70328","type":"journal-article","created":{"date-parts":[[2026,7,10]],"date-time":"2026-07-10T14:00:48Z","timestamp":1783692048000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Alcohol or Stress Exposure During Late Adolescence Impairs Risk Assessment Later in Life, Disrupting the Ethological Structure of Behavior in Mice"],"prefix":"10.1111","volume":"50","author":[{"given":"Beatriz Deo","family":"Sorigotto","sequence":"first","affiliation":[{"name":"Programa de P\u00f3s\u2010Gradua\u00e7\u00e3o em Psicobiologia, Escola Paulista de Medicina Universidade Federal de S\u00e3o Paulo  S\u00e3o Paulo Brazil"},{"name":"Departamento de Psicobiologia, Escola Paulista de Medicina Universidade Federal de S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabel Marian Hartmann","family":"de Quadros","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s\u2010Gradua\u00e7\u00e3o em Psicobiologia, Escola Paulista de Medicina Universidade Federal de S\u00e3o Paulo  S\u00e3o Paulo Brazil"},{"name":"Departamento de Psicobiologia, Escola Paulista de Medicina Universidade Federal de S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4528-2430","authenticated-orcid":false,"given":"Karina Possa","family":"Abrahao","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s\u2010Gradua\u00e7\u00e3o em Psicobiologia, Escola Paulista de Medicina Universidade Federal de S\u00e3o Paulo  S\u00e3o Paulo Brazil"},{"name":"Departamento de Fisiologia, Escola Paulista de Medicina Universidade Federal de S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,7,10]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbr.2014.02.032"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0149\u20107634(01)00053\u20107"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.alcohol.2018.04.003"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jneumeth.2013.04.022"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.physbeh.2004.03.014"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00213\u2010016\u20104395\u20106"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2018\u201000220"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0091\u20103057(02)00958\u20109"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-0348-6419-0_13"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neubiorev.2010.10.016"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-94-009-2403-1"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/0091\u20103057(93)90185\u2010v"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0014\u20102999(03)01274\u20103"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41567\u2010018\u20100093\u20100"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0008528"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neubiorev.2005.04.017"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010018\u201028381\u20102"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/adb.12164"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/0091\u20103057(80)90067\u20102"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/0091\u20103057(94)90472\u20103"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2015.10.025"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0166\u20104328(96)02245\u20100"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/2041\u2010210x.12584"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/laban1005\u201039"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/s002130050645"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuropharm.2009.05.007"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.7554\/elife.93485"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jesp.2024.104621"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1152\/jn.91039.2008"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1007\/978\u20101\u20104939\u20108994\u20102_4"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0361\u20109230(01)00569\u2010x"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0031\u20106997(25)06843\u20107"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbr.2004.09.004"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pcbi.1000229"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neubiorev.2023.105274"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1093\/ijnp\/pyae006"},{"key":"e_1_2_9_38_1","doi-asserted-by":"crossref","DOI":"10.17226\/25801","volume-title":"Guide for the Care and Use of Laboratory Animals","author":"National Research Council (US) Committee for the Update of the Guide for the Care and Use of Laboratory Animals","year":"2011"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1037\/0735\u20107044.115.2.429"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010019\u201056408\u20109"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.physbeh.2026.115277"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2023.10.024"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.3389\/fendo.2016.00134"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pbb.2006.01.007"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13293\u2010023\u201000565\u20100"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0149\u20107634(96)00058\u20109"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopsycho.2017.03.004"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.beproc.2019.103952"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.1038\/npp.2014.151"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pneurobio.2013.04.001"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.1038\/npp.2012.69"},{"key":"e_1_2_9_52_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopsych.2004.02.032"},{"key":"e_1_2_9_53_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jadohealth.2012.05.006"},{"key":"e_1_2_9_54_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrn.2018.10"},{"key":"e_1_2_9_55_1","doi-asserted-by":"publisher","DOI":"10.1093\/ijnp\/pyac038"},{"key":"e_1_2_9_56_1","doi-asserted-by":"publisher","DOI":"10.1037\/bne0000612"},{"key":"e_1_2_9_57_1","doi-asserted-by":"publisher","DOI":"10.3791\/104"},{"key":"e_1_2_9_58_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014\u20102999(00)00569\u20100"},{"key":"e_1_2_9_59_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0165\u20103806(98)00111\u20104"},{"key":"e_1_2_9_60_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jneumeth.2010.09.008"},{"key":"e_1_2_9_61_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnbeh.2020.00045"},{"key":"e_1_2_9_62_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnbeh.2023.1140724"},{"key":"e_1_2_9_63_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00213\u2010009\u20101475\u2010x"},{"key":"e_1_2_9_64_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41386\u2010021\u201001014\u20105"},{"key":"e_1_2_9_65_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pbb.2007.04.013"}],"container-title":["Alcohol, Clinical and Experimental Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/acer.70328","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/acer.70328","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/acer.70328","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T08:45:04Z","timestamp":1785833104000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/acer.70328"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7]]},"references-count":64,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2026,7]]}},"alternative-id":["10.1111\/acer.70328"],"URL":"https:\/\/doi.org\/10.1111\/acer.70328","archive":["Portico"],"relation":{},"ISSN":["2993-7175","2993-7175"],"issn-type":[{"value":"2993-7175","type":"print"},{"value":"2993-7175","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7]]},"assertion":[{"value":"2026-01-09","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-05-15","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-07-10","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70328"}}