{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,27]],"date-time":"2026-06-27T04:45:25Z","timestamp":1782535525140,"version":"3.54.5"},"reference-count":36,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2026,6,5]],"date-time":"2026-06-05T00:00:00Z","timestamp":1780617600000},"content-version":"vor","delay-in-days":4,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Alcohol, Clinical and Experimental Research"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Perinatal exposure to alcohol is a critical risk factor for long\u2010term skeletal health in humans and has been demonstrated in rodent models. Early postnatal alcohol gavage exposure in mice is used to study third trimester toxicity in humans. However, further research is required to fully characterize the effects of alcohol on bone molecular composition and distinguish them from the alterations associated with gavage\u2010related stress in mouse models.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Female mouse pups were assigned to untreated control (C), gavage control (GC), and alcohol\u2010treated (A) groups. Between postnatal days 3 and 20, the A group received ethanol (3.0\u2009g\/kg\/day) by intragastric gavage, while the GC group underwent the same gavage procedure without any solution (neither milk nor ethanol). At postnatal day 90, femoral bone molecular composition and bone metabolism were evaluated by attenuated total reflection Fourier\u2010transform infrared (ATR\u2010FTIR) spectroscopy and biochemical studies.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Several ATR\u2010FTIR\u2010derived matrix and mineral\u2010related alterations, including reduced total protein\u2010related indices, lower collagen cross\u2010link ratio, reduced mineral\u2010to\u2010matrix ratio, and increased crystallinity, were observed in both GC and A groups relative to untreated controls, indicating a substantial long\u2010term influence of the gavage procedure itself. Compared with the GC, the A group showed additional selective differences, most notably reduced relative carbonate content and increased CTX\u2010I levels. In the C group, serum 25(OH)D, PTH, and calcitonin levels differed from both GC and A groups, whereas osteocalcin was increased in the GC group relative to the C group, and no significant difference was observed between the GC and A groups.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Prolonged neonatal gavage was associated with substantial long\u2010term changes in bone molecular composition and several circulating bone\u2010related hormonal markers. Against this background, postnatal alcohol exposure was associated with additional selective differences, particularly in CTX\u2010I and relative carbonate content. These findings highlight the importance of discriminating between the effect of the procedure and the effect of alcohol in developmental models based on repeated neonatal gavage.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/acer.70331","type":"journal-article","created":{"date-parts":[[2026,6,5]],"date-time":"2026-06-05T11:54:58Z","timestamp":1780660498000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["The Effects of Early Postnatal Alcohol Exposure on Bone Molecular Composition in a Mouse Model"],"prefix":"10.1111","volume":"50","author":[{"given":"Ilknur","family":"Dursun","sequence":"first","affiliation":[{"name":"Department of Physiology, Faculty of Medicine Istanbul Health and Technology University  Istanbul Turkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Birsen","family":"Elibol","sequence":"additional","affiliation":[{"name":"Department of Medical Biology, Faculty of Medicine Istanbul Medeniyet University  Istanbul Turkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7844-6876","authenticated-orcid":false,"given":"Sebnem Garip","family":"Ustaoglu","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Faculty of Pharmacy Istanbul Aydin University  Istanbul Turkiye"},{"name":"Department of Medical Biochemistry, Faculty of Medicine Altinbas University  Istanbul Turkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,6,5]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2022.121102"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms23010044"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.saa.2020.118311"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/JBIO.202400066"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/1873\u20103468.14259"},{"key":"e_1_2_11_7_1","doi-asserted-by":"crossref","unstructured":"Boskey A. andN. 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