{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T15:01:55Z","timestamp":1790694115892,"version":"4.1.0"},"reference-count":39,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T00:00:00Z","timestamp":1788739200000},"content-version":"vor","delay-in-days":6,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100000027","name":"National Institute on Alcohol Abuse and Alcoholism","doi-asserted-by":"publisher","award":["K23AA024704"],"award-info":[{"award-number":["K23AA024704"]}],"id":[{"id":"10.13039\/100000027","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000027","name":"National Institute on Alcohol Abuse and Alcoholism","doi-asserted-by":"publisher","award":["R01AA031640"],"award-info":[{"award-number":["R01AA031640"]}],"id":[{"id":"10.13039\/100000027","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["P20GM130414"],"award-info":[{"award-number":["P20GM130414"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["3P20GM130414\u201004S1"],"award-info":[{"award-number":["3P20GM130414\u201004S1"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Alcohol, Clinical and Experimental Research"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Alcohol consumption is a major contributor to global chronic disease, with growing evidence indicating health risks even at low levels of intake. However, mechanistic understanding of these risks relies heavily on preclinical models and observational data, leaving a critical gap in controlled experimental evidence regarding how alcohol perturbs human biological systems in\u00a0vivo.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      The present study utilized plasma samples from a randomized, placebo\u2010controlled trial to evaluate the effects of low\u2010dose (0.35\u2009g\/kg) and moderate\u2010dose (0.60\u2009g\/kg) alcohol on disease\u2010relevant biomarkers in 32 healthy adults (mean age\u2009=\u200925.0\u2009\u00b1\u20093.8\u2009years; 21 female\/11 male), characterized by light (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u200915) or heavy (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u200917) drinking. This design enabled evaluation of effects across dose, timescale, and drinking history, as well as assessment of their interactions. Plasma was collected at prebeverage baseline and hourly for 4\u2009h afterward. Immunoassays quantified 10 disease\u2010related biomarkers: adiponectin, angiogenin, D\u2010dimer, high\u2010sensitivity C\u2010reactive protein (hsCRP), Intercellular Adhesion Molecule\u20101 (ICAM\u20101), Lipocalin\u20102 (LCN2), Matrix Metalloproteinase\u20107 (MMP\u20107), Matrix Metalloproteinase\u20109 (MMP\u20109), soluble Receptor for Advanced Glycation End\u2010products (sRAGE), and Triggering Receptor Expressed on Myeloid cells 2 (TREM2).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Main effects of group indicated that even in this young healthy sample, heavy drinking status was associated with higher levels of adiponectin, angiogenin, ICAM\u20101, LCN2, and sRAGE, a profile suggesting altered vascular and metabolic activity. Acute alcohol administration induced changes in sRAGE and hsCRP. Specifically, moderate\u2010dose alcohol triggered an increase in the immunoglobulin sRAGE, which may reflect an acute compensatory response to inflammation and\/or oxidative stress. Compared to placebo, hsCRP was lower in the low\u2010dose alcohol condition; however, this finding should be interpreted in light of CRP biology. MMP\u20107, MMP\u20109, and LCN2 showed time\u2010dependent fluctuations that were independent of experimental condition, highlighting the critical importance of placebo\u2010controlled designs to account for diurnal\/postprandial variation in immune biomarkers.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Findings provide translational evidence that alcohol is associated with multisystem biomarker changes relevant to chronic disease and that alcohol\u2010related biomarker perturbations vary by dose and chronicity.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/acer.70362","type":"journal-article","created":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T14:02:42Z","timestamp":1788789762000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Effects of Acute Low\u2010 and Moderate\u2010Dose Alcohol on Chronic Disease\u2010Related Biomarkers in Healthy Light and Heavy Drinkers"],"prefix":"10.1111","volume":"50","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3166-2336","authenticated-orcid":false,"given":"Mollie A.","family":"Monnig","sequence":"first","affiliation":[{"name":"Center for Alcohol and Addiction Studies Brown University  Providence Rhode Island USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samantha E.","family":"Clark","sequence":"additional","affiliation":[{"name":"Center for Alcohol and Addiction Studies Brown University  Providence Rhode Island USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter M.","family":"Monti","sequence":"additional","affiliation":[{"name":"Center for Alcohol and Addiction Studies Brown University  Providence Rhode Island 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