{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T10:54:28Z","timestamp":1784544868430,"version":"3.55.0"},"reference-count":50,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2014,11,6]],"date-time":"2014-11-06T00:00:00Z","timestamp":1415232000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004737","name":"National Health Research Institutes, Taiwan","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100004737","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Addiction Biology"],"published-print":{"date-parts":[[2016,3]]},"abstract":"Abstract<\/jats:title>In this study, methamphetamine (M<\/jats:styled-content>eth)\u2010 and glutamate (G<\/jats:styled-content>lu)\u2010mediated intracellularCa++<\/jats:sup><\/jats:roman><\/jats:styled-content>(Ca++<\/jats:sup><\/jats:roman><\/jats:styled-content>i<\/jats:italic>) signals were examined in real time in primary cortical neurons overexpressing an intracellularCa++<\/jats:sup><\/jats:roman><\/jats:styled-content>probe,GCaMP5<\/jats:styled-content>, by adeno\u2010associated viral (AAV<\/jats:styled-content>) serotype 1. Binding ofCa++<\/jats:sup><\/jats:roman><\/jats:styled-content>toGCaMP<\/jats:styled-content>increased green fluorescence intensity in cells. BothM<\/jats:styled-content>eth andG<\/jats:styled-content>lu induced a rapid increase inCa++<\/jats:sup><\/jats:roman><\/jats:styled-content>i<\/jats:italic>, which was blocked byMK<\/jats:styled-content>801, suggesting thatM<\/jats:styled-content>eth enhancedCa++<\/jats:sup><\/jats:roman><\/jats:styled-content>i<\/jats:italic>throughG<\/jats:styled-content>lu receptor in neurons. TheM<\/jats:styled-content>eth\u2010mediatedCa++<\/jats:sup><\/jats:roman><\/jats:styled-content>signal was also blocked byMg++<\/jats:sup><\/jats:roman><\/jats:styled-content>, lowCa++<\/jats:sup><\/jats:roman><\/jats:styled-content>or theL<\/jats:styled-content>\u2010typeCa++<\/jats:sup><\/jats:roman><\/jats:styled-content>channel inhibitor nifedipine. The ryanodine receptor inhibitor dantrolene did not alter the initialCa++<\/jats:sup><\/jats:roman><\/jats:styled-content>influx but partially reduced the peak ofCa++<\/jats:sup><\/jats:roman><\/jats:styled-content>i<\/jats:italic>. These data suggest thatM<\/jats:styled-content>eth enhancedCa++<\/jats:sup><\/jats:roman><\/jats:styled-content>influx through membraneCa++<\/jats:sup><\/jats:roman><\/jats:styled-content>channels, which then triggered the release ofCa++<\/jats:sup><\/jats:roman><\/jats:styled-content>from the endoplasmic reticulum in the cytosol.AAV<\/jats:styled-content>\u2010GCaMP<\/jats:styled-content>5 was also injected to the parietal cortex of adult rats. Administration ofM<\/jats:styled-content>eth enhanced fluorescence in the ipsilateral cortex. Using immunohistochemistry,M<\/jats:styled-content>eth\u2010induced green fluorescence was found in theNeuN<\/jats:styled-content>\u2010containing cells in the cortex, suggesting thatM<\/jats:styled-content>eth increasedCa++<\/jats:sup><\/jats:roman><\/jats:styled-content>in neuronsin vivo<\/jats:italic>. In conclusion, we have usedin vitro<\/jats:italic>andin vivo<\/jats:italic>techniques to demonstrate a rapid increase ofCa++<\/jats:sup><\/jats:roman><\/jats:styled-content>i<\/jats:italic>byM<\/jats:styled-content>eth in cortical neurons through overexpression ofGCaMP<\/jats:styled-content>5. AsM<\/jats:styled-content>eth induces behavioral responses and neurotoxicity throughCa++<\/jats:sup><\/jats:roman><\/jats:styled-content>i<\/jats:italic>, modulation ofCa++<\/jats:sup><\/jats:roman><\/jats:styled-content>i<\/jats:italic>may be useful to reduceM<\/jats:styled-content>eth\u2010related reactions.<\/jats:p>","DOI":"10.1111\/adb.12193","type":"journal-article","created":{"date-parts":[[2014,11,6]],"date-time":"2014-11-06T08:02:48Z","timestamp":1415260968000},"page":"255-266","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Methamphetamine induces a rapid increase of intracellularCa++<\/sup><\/scp><\/scp>levels in neurons overexpressingGCaMP<\/scp>5"],"prefix":"10.1111","volume":"21","author":[{"given":"Seong\u2010Jin","family":"Yu","sequence":"first","affiliation":[{"name":"Center for Neuropsychiatric Research National Health Research Institutes Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kou\u2010Jen","family":"Wu","sequence":"additional","affiliation":[{"name":"Center for Neuropsychiatric Research National Health Research Institutes Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eun K.","family":"Bae","sequence":"additional","affiliation":[{"name":"Center for Neuropsychiatric Research National Health Research Institutes Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Man\u2010Jung","family":"Hsu","sequence":"additional","affiliation":[{"name":"Center for Neuropsychiatric Research National Health Research Institutes Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher T.","family":"Richie","sequence":"additional","affiliation":[{"name":"National Institute on Drug Abuse NIH Baltimore MD USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brandon K.","family":"Harvey","sequence":"additional","affiliation":[{"name":"National Institute on Drug Abuse NIH Baltimore MD USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yun","family":"Wang","sequence":"additional","affiliation":[{"name":"Center for Neuropsychiatric Research National Health Research Institutes Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,11,6]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.2601-12.2012"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3940(95)11765-O"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0015643"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF03033567"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuropharm.2008.04.003"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.3791\/50357"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-8993(00)02868-7"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1020769816332"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0169-328X(01)00184-X"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1471-4159.1996.66051949.x"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.2007.05142.x"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M805298200"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuro.2013.08.009"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1208\/aapsj080480"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1159\/000028195"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2007.10.007"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0893-133X(99)00116-5"},{"key":"e_1_2_7_19_1","doi-asserted-by":"crossref","first-page":"1016","DOI":"10.1016\/S0022-3565(24)29628-2","article-title":"Protein kinase C and intracellular calcium are required for amphetamine\u2010mediated dopamine release via the norepinephrine transporter in undifferentiated PC12 cells","volume":"297","author":"Kantor L","year":"2001","journal-title":"J Pharmacol Exp Ther"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1124\/jpet.104.071068"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.16-13-04231.1996"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10895-013-1312-9"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.expneurol.2007.05.023"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2010.11.062"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.2009.05998.x"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.4383-09.2010"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0015193"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0001796"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01245234"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/84397"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(94)91163-0"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-8993(03)02267-4"},{"key":"e_1_2_7_33_1","doi-asserted-by":"crossref","first-page":"6348","DOI":"10.1523\/JNEUROSCI.19-15-06348.1999","article-title":"L\u2010type Ca(2+) channels are essential for glutamate\u2010mediated CREB phosphorylation and c\u2010fos gene expression in striatal neurons","volume":"19","author":"Rajadhyaksha A","year":"1999","journal-title":"J Neurosci"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1098-2396(199710)27:2<95::AID-SYN1>3.0.CO;2-6"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2011.08.013"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.2011.07387.x"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1017\/S1740925X10000219"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2009.10.124"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms3170"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/0896-6273(95)90294-5"},{"key":"e_1_2_7_41_1","first-page":"81","article-title":"Effects of calcium antagonists on the cocaine\u2010 and methamphetamine\u2010induced conditioned place preference","volume":"27","author":"Suzuki T","year":"1992","journal-title":"Arukoru Kenkyuto Yakubutsu Ison"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0005546"},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.1398"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-201X.1971.tb10998.x"},{"key":"e_1_2_7_45_1","doi-asserted-by":"publisher","DOI":"10.1097\/00001756-200004070-00031"},{"key":"e_1_2_7_46_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.21-23-09414.2001"},{"key":"e_1_2_7_47_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1471-4159.2000.0751634.x"},{"key":"e_1_2_7_48_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10571-004-6927-6"},{"key":"e_1_2_7_49_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00213-011-2595-7"},{"key":"e_1_2_7_50_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0081750"},{"key":"e_1_2_7_51_1","first-page":"361","article-title":"Inhibition of methamphetamine\u2010induced apoptosis by the calcium channel blocker verapamil in rat cerebellar neurons","volume":"36","author":"Zhou JL","year":"2004","journal-title":"Beijing Da Xue Xue Bao"}],"container-title":["Addiction Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fadb.12193","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/adb.12193","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,5,13]],"date-time":"2025-05-13T15:08:45Z","timestamp":1747148925000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/adb.12193"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,11,6]]},"references-count":50,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2016,3]]}},"alternative-id":["10.1111\/adb.12193"],"URL":"https:\/\/doi.org\/10.1111\/adb.12193","archive":["Portico"],"relation":{},"ISSN":["1355-6215","1369-1600"],"issn-type":[{"value":"1355-6215","type":"print"},{"value":"1369-1600","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,11,6]]},"assertion":[{"value":"2014-11-06","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}