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Here, we aim to identify the role of miR\u2010128 on METH\u2010induced locomotor sensitization of male mice. We identified a significant increase of miR\u2010128 in the NAc of mice upon repeated\u2010intermittent METH exposure but not acute METH administration. Microinjection of adeno\u2010associated virus (AAV)\u2010miR\u2010128 over\u2010expression and inhibition constructs into the NAc of mice resulted in enhanced METH\u2010induced locomotor sensitization and attenuated effects of METH respectively. Isobaric tags for relative and absolute quantification (iTRAQ) technology and ingenuity pathway analysis (IPA) were carried out to uncover the potential molecular mechanisms underlying miR\u2010128\u2010regulated METH sensitization. Differentially expressed proteins, including 25 potential targets for miR\u2010128 were annotated in regulatory pathways that modulate dendritic spines, synaptic transmission and neuritogenesis. Of which, Arf6, Cpeb3 and Nlgn1, were found to be participating in miR\u2010128\u2010regulated METH sensitization. Consistently, METH\u2010induced abnormal changes of Arf6, Cpeb3 and Nlgn1 in the NAc of mice were also detected by qPCR and validated by western blot analysis. Thus, miR\u2010128 may contribute to METH sensitization through controlling neuroplasticity. Our study suggested miR\u2010128 was an important regulator of METH\u2010 induced sensitization and also provided the potential molecular networks of miR\u2010128 in regulating METH\u2010induced sensitization.<\/jats:p>","DOI":"10.1111\/adb.12881","type":"journal-article","created":{"date-parts":[[2020,2,14]],"date-time":"2020-02-14T13:53:01Z","timestamp":1581688381000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["Regulation of miR\u2010128 in the nucleus accumbens affects methamphetamine\u2010induced behavioral sensitization by modulating proteins involved in neuroplasticity"],"prefix":"10.1111","volume":"26","author":[{"given":"Jiaqi","family":"Li","sequence":"first","affiliation":[{"name":"College of Forensic Medicine  Xi'an Jiaotong University Health Science Center Xi'an Shaanxi 710061 China"},{"name":"The Key Laboratory of Health Ministry for Forensic Medicine, Xi'an Jiaotong University  Shaanxi 710061 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