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First, we used epigenomic deconvolution to perform cell\u2010type\u2010specific methylome\u2010wide association studies within subpopulations of granulocytes\/T\u2010cells\/B\u2010cells\/monocytes in 1132 blood samples. Blood findings were then examined for overlap with AUD\u2010related associations with methylation and hydroxymethylation in 50 human post\u2010mortem brain samples. Follow\u2010up analyses investigated if overlapping findings mediated AUD\u2010associated transcription changes in the same brain samples. Lastly, we replicated our blood findings in an independent sample of 412 individuals and aimed to replicate published alcohol methylation findings using our results. Cell\u2010type\u2010specific analyses in blood identified methylome\u2010wide significant associations in monocytes and T\u2010cells. The monocyte findings were significantly enriched for AUD\u2010related methylation and hydroxymethylation in brain. Hydroxymethylation in specific sites mediated AUD\u2010associated transcription in the same brain samples. As part of the most comprehensive methylation study of AUD to date, this work involved the first cell\u2010type\u2010specific methylation study of AUD conducted in blood, identifying and replicating a finding in\n                    <jats:italic>DLGAP1<\/jats:italic>\n                    that may be a blood\u2010based biomarker of AUD. In this first study to consider the role of hydroxymethylation in AUD, we found evidence for a novel mechanism for cognitive deficits associated with AUD. Our results suggest promising new avenues for AUD research.\n                  <\/jats:p>","DOI":"10.1111\/adb.13114","type":"journal-article","created":{"date-parts":[[2021,11,18]],"date-time":"2021-11-18T01:03:02Z","timestamp":1637197382000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Dual methylation and hydroxymethylation study of alcohol use disorder"],"prefix":"10.1111","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3193-6923","authenticated-orcid":false,"given":"Shaunna L.","family":"Clark","sequence":"first","affiliation":[{"name":"Department of Psychiatry &amp; Behavioral Sciences Texas A&amp;M University  Bryan Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robin F.","family":"Chan","sequence":"additional","affiliation":[{"name":"Center for Biomarker Research and Precision Medicine Virginia Commonwealth University  Richmond Virginia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min","family":"Zhao","sequence":"additional","affiliation":[{"name":"Center for Biomarker Research and Precision Medicine Virginia Commonwealth University  Richmond Virginia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lin Y.","family":"Xie","sequence":"additional","affiliation":[{"name":"Center for Biomarker Research and Precision Medicine Virginia Commonwealth University  Richmond Virginia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William E.","family":"Copeland","sequence":"additional","affiliation":[{"name":"Department of Psychiatry University of Vermont  Burlington Vermont USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brenda W. J. H.","family":"Penninx","sequence":"additional","affiliation":[{"name":"Department of Psychiatry Amsterdam UMC, Vrije Universiteit  Amsterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karolina A.","family":"Aberg","sequence":"additional","affiliation":[{"name":"Center for Biomarker Research and Precision Medicine Virginia Commonwealth University  Richmond Virginia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edwin J. C. G.","family":"van den Oord","sequence":"additional","affiliation":[{"name":"Center for Biomarker Research and Precision Medicine Virginia Commonwealth University  Richmond Virginia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,11,17]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuropharm.2013.04.004"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41380-019-0449-6"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/acer.13138"},{"issue":"9","key":"e_1_2_9_5_1","article-title":"Epigenome\u2010wide association study and multi\u2010tissue replication of individuals with alcohol use disorder: evidence for abnormal glucocorticoid signaling pathway gene regulation","volume":"87","author":"Lohoff FW","year":"2020","journal-title":"Mol 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