{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T16:10:17Z","timestamp":1785255017587,"version":"3.55.0"},"reference-count":51,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2021,11,25]],"date-time":"2021-11-25T00:00:00Z","timestamp":1637798400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["P20GM125528"],"award-info":[{"award-number":["P20GM125528"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000049","name":"National Institute on Aging","doi-asserted-by":"publisher","award":["P30AG050911"],"award-info":[{"award-number":["P30AG050911"]}],"id":[{"id":"10.13039\/100000049","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000049","name":"National Institute on Aging","doi-asserted-by":"publisher","award":["R01AG052606"],"award-info":[{"award-number":["R01AG052606"]}],"id":[{"id":"10.13039\/100000049","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000049","name":"National Institute on Aging","doi-asserted-by":"publisher","award":["R01AG059430"],"award-info":[{"award-number":["R01AG059430"]}],"id":[{"id":"10.13039\/100000049","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000026","name":"National Institute on Drug Abuse","doi-asserted-by":"publisher","award":["R01DA032701"],"award-info":[{"award-number":["R01DA032701"]}],"id":[{"id":"10.13039\/100000026","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000738","name":"U.S. Department of Veterans Affairs","doi-asserted-by":"publisher","award":["I01BX003906"],"award-info":[{"award-number":["I01BX003906"]}],"id":[{"id":"10.13039\/100000738","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100001298","name":"Presbyterian Health Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100001298","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Addiction Biology"],"published-print":{"date-parts":[[2022,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Dopamine neurons in the substantia nigra (SN) and ventral tegmental area (VTA) play a central role in the reinforcing properties of abused drugs including methamphetamine and cocaine. Chronic effects of psychostimulants in the SN\/VTA also involve non\u2010dopaminergic transmitters, including glutamate and the stress\u2010related peptide corticotropin\u2010releasing factor (CRF). In the SN\/VTA, astrocytes express a variety of membrane\u2010bound neurotransmitter receptors and transporters that influence neurotransmission. CRF receptor type 2 (CRF2) activity in the VTA is important for stress\u2010induced relapse and drug\u2010seeking behaviour, but the localization of its effects is incompletely understood. Here, we first identified CRF2 transcript in astrocytes of the SN\/VTA using RNA\u2010Seq in Aldh1l1;NuTRAP mice and confirmed it using in situ hybridization (RNAscope) in wild\u2010type mice. We then used immunofluorescence to quantify the astrocytic marker protein S100\u03b2, glial\u2010specific glutamate\/aspartate transporter GLAST, and CRF2 in the SN\/VTA following 12\u2009days of treatment (i.p.) with methamphetamine (3\u2009mg\/kg), cocaine (10\u00a0mg\/kg), or saline. We observed a significant decrease in GLAST immunofluorescence in brains of psychostimulant treated mice compared with saline controls. In addition, we observed increased labelling of CRF2 in drug treated groups, a decrease in the number of S100\u03b2 positive cells, and an increase of co\u2010staining of CRF2 with both S100\u03b2 and tyrosine hydroxylase (dopamine neurons). Our results suggest a significant interaction between CRF2, GLAST, and astrocytes in the midbrain that emerges with repeated exposure to psychostimulants. These findings provide rationale for future investigation of astrocyte\u2010based strategies for altering cellular and circuit function in response to stress and drug exposure.<\/jats:p>","DOI":"10.1111\/adb.13120","type":"journal-article","created":{"date-parts":[[2021,11,26]],"date-time":"2021-11-26T11:29:19Z","timestamp":1637926159000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Repeated cocaine or methamphetamine treatment alters astrocytic CRF2 and GLAST expression in the ventral midbrain"],"prefix":"10.1111","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3971-5946","authenticated-orcid":false,"given":"Amanda L.","family":"Sharpe","sequence":"first","affiliation":[{"name":"Department of Pharmaceutical Sciences University of Oklahoma College of Pharmacy  Oklahoma City Oklahoma USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marta","family":"Trzeciak","sequence":"additional","affiliation":[{"name":"Aging and Metabolism Research Program Oklahoma Medical Research Foundation  Oklahoma City Oklahoma USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4625-659X","authenticated-orcid":false,"given":"Nicole L.","family":"Eliason","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Sciences University of Oklahoma College of Pharmacy  Oklahoma City Oklahoma USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7570-4895","authenticated-orcid":false,"given":"Harris E.","family":"Blankenship","sequence":"additional","affiliation":[{"name":"Aging and Metabolism Research Program Oklahoma Medical Research Foundation  Oklahoma City Oklahoma USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5027-9909","authenticated-orcid":false,"given":"Bre' Ana M.","family":"Byrd","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Sciences University of Oklahoma College of Pharmacy  Oklahoma City Oklahoma USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Phillip D.","family":"Douglas","sequence":"additional","affiliation":[{"name":"Aging and Metabolism Research Program Oklahoma Medical Research Foundation  Oklahoma City Oklahoma USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7027-999X","authenticated-orcid":false,"given":"Willard M.","family":"Freeman","sequence":"additional","affiliation":[{"name":"Genes and Human Disease Research Program Oklahoma Medical Research Foundation  Oklahoma City Oklahoma USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1574-530X","authenticated-orcid":false,"given":"Michael J.","family":"Beckstead","sequence":"additional","affiliation":[{"name":"Aging and Metabolism Research Program Oklahoma Medical Research Foundation  Oklahoma City Oklahoma USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,11,25]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2017.06.020"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-09131-y"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0813415106"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainres.2009.09.101"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/npp.2015.142"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00213-005-2180-z"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.0955-05.2005"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s002130050150"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00213-007-0782-3"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1460-9568.2006.04792.x"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(03)00461-6"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2007.12.029"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2007.150078"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.4773-08.2009"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/npp.2009.25"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.0911-13.2014"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/s42003-020-01418-x"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2014.085"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2016.12.087"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-27293-5"},{"key":"e_1_2_9_22_1","first-page":"375","article-title":"Paxinos and Franklin's the mouse brain in stereotaxic coordinates","author":"Paxinos G","year":"2019","journal-title":"Academic Press"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.21033"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/npp.2015.49"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00213-015-4144-2"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopsych.2016.02.029"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2011.07.101"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/jnc.15340"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1111\/ejn.13113"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neubiorev.2018.04.014"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.21576"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.1393-11.2011"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1093\/ijnp\/pyu073"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1038\/npp.2016.138"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1002\/1096-9861(20001211)428:2<191::AID-CNE1>3.0.CO;2-U"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrn2274"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrn.2016.165"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature01476"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.psych.56.091103.070229"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1177\/1073858413520347"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41386-021-01090-7"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.7916485"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1152\/jn.2000.83.3.1307"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1038\/cdd.2009.131"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1116916"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuint.2007.06.026"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tips.2006.10.008"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainresrev.2007.05.004"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2014.10.017"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.1152\/jn.00094.2012"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.1988.tb03036.x"},{"key":"e_1_2_9_52_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00213-011-2433-y"}],"container-title":["Addiction Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/adb.13120","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/adb.13120","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/adb.13120","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,22]],"date-time":"2024-01-22T08:44:40Z","timestamp":1705913080000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/adb.13120"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,11,25]]},"references-count":51,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2022,3]]}},"alternative-id":["10.1111\/adb.13120"],"URL":"https:\/\/doi.org\/10.1111\/adb.13120","archive":["Portico"],"relation":{},"ISSN":["1355-6215","1369-1600"],"issn-type":[{"value":"1355-6215","type":"print"},{"value":"1369-1600","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,11,25]]},"assertion":[{"value":"2021-05-03","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-11-04","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-11-25","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e13120"}}