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Here, we report the development of an accessible, two\u2010choice self\u2010administration zebrafish assay (SAZA) to study the neurobiology of addiction. Using this assay, we first demonstrated that, although zebrafish avoid higher concentrations of alcohol, they are attracted to low concentrations. Pre\u2010exposure to alcohol did not change this relative preference, but acute exposure to an alcohol deterrent approved for human use decreased alcohol self\u2010administration. A pigment mutant used in whole\u2010brain imaging studies displayed a similar relative alcohol preference profile; however, mutants in\n                    <jats:italic>CCSER1<\/jats:italic>\n                    , a gene associated with alcohol dependence in human genetic studies, showed a reversal in relative preference. The presence of a biphasic response (hormesis) in zebrafish validated a key aspect of vertebrate responses to alcohol. SAZA adds a new dimension for discovering novel alcohol deterrents and studying the neurogenetics of addiction using the zebrafish.\n                  <\/jats:p>","DOI":"10.1111\/adb.13126","type":"journal-article","created":{"date-parts":[[2022,1,14]],"date-time":"2022-01-14T02:15:43Z","timestamp":1642126543000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Contingent stimulus delivery assay for zebrafish reveals a role for CCSER1 in alcohol preference"],"prefix":"10.1111","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5791-8707","authenticated-orcid":false,"given":"Fatima Megala","family":"Nathan","sequence":"first","affiliation":[{"name":"Yale\u2010NUS College  Singapore 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