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Despite the increasing use of NBOMes for diagnostic, research and recreational purposes, only a limited number of studies have focussed on their in vivo effect. Here, we investigated pharmacokinetics, systemic toxicity, thermoregulation in individually and group\u2010housed animals, and acute behavioural effects after subcutaneous administration of 2,5\u2010dimethoxy\u20104\u2010(2\u2010((2\u2010methoxybenzyl)amino)ethyl)benzonitrile (25CN\u2010NBOMe; 0.2, 1, and 5\u2009mg\/kg) in Wistar rats. Drug concentration peaked 1\u2009h after the administration of 5\u2009mg\/kg in both blood serum and brain tissue with a half\u2010life of 1.88 and 2.28\u2009h, respectively. According to Organisation for Economic Co\u2010operation and Development 423 toxicity assay, the drug is classified into category 3 with a lethal dose of 300\u2009mg\/kg and an estimated LD50 value of 200\u2009mg\/kg. Histological examination of organs collected from rats injected with the lethal dose revealed subtle pathological changes, highly suggestive of acute cardiovascular arrest due to malignant arrhythmia. Altered thermoregulation after 5\u2009mg\/kg was demonstrated by reduced body temperature in individually housed rats (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.01). Behavioural effects assessed by the Open Field test and Prepulse Inhibition of Startle Response revealed that the two lower doses (0.2 and 1\u2009mg\/kg) caused a reduction in locomotor activity (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.01), increased anxiety (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05) and 5\u2009mg\/kg additionally impaired sensorimotor gating (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001). In summary, 25CN\u2010NBOMe readily passes the blood\u2013brain barrier and exhibits a moderate level of toxicity and behavioural effect comparable with other NBOMes.<\/jats:p>","DOI":"10.1111\/adb.13216","type":"journal-article","created":{"date-parts":[[2022,8,4]],"date-time":"2022-08-04T10:39:39Z","timestamp":1659609579000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Pharmacokinetics, systemic toxicity, thermoregulation and acute behavioural effects of 25CN\u2010NBOMe"],"prefix":"10.1111","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1653-822X","authenticated-orcid":false,"given":"Kl\u00e1ra","family":"\u0160\u00edchov\u00e1","sequence":"first","affiliation":[{"name":"Psychedelics Research Centre National Institute of Mental Health  Klecany Czech 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