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In rats, cue\u2010induced craving progressively intensifies (\u2018incubates\u2019) during withdrawal from extended\u2010access self\u2010administration of several classes of misused drugs, including the psychostimulants cocaine and methamphetamine. For these psychostimulants, incubation is associated with strengthening of excitatory synapses in the nucleus accumbens (NAc) through incorporation of \u03b1\u2010amino\u20103\u2010hydroxy\u20105\u2010methyl\u20104\u2010isoxazole propionic acid receptors that lack the GluA2 subunit and are therefore Ca<jats:sup>2+<\/jats:sup>\u2010permeable (CP\u2010AMPARs). Once CP\u2010AMPAR upregulation occurs, their stimulation is required for expression of incubation. It is not known if a similar mechanism contributes to incubation of oxycodone craving. Using male rats, we established that incubation occurs by withdrawal day (WD) 15 and persists through WD30. Then, using cell\u2010surface biotinylation, we found that surface levels of the AMPAR subunit GluA1 but not GluA2 are elevated in NAc core and shell of oxycodone rats on WD15, although this wanes by WD30. Next, using intra\u2010NAc injection of the selective CP\u2010AMPAR antagonist Naspm before a seeking test, we demonstrate that CP\u2010AMPAR blockade in either subregion decreases oxycodone seeking on WD15 or WD30 (after incubation), but not WD1, and has no effect in saline self\u2010administering animals. The Naspm results suggest CP\u2010AMPARs persist in synapses through WD30 even if total cell surface levels wane. These results suggest that a common neurobiological mechanism contributes to expression of incubation of craving for oxycodone and psychostimulants.<\/jats:p>","DOI":"10.1111\/adb.13237","type":"journal-article","created":{"date-parts":[[2022,10,21]],"date-time":"2022-10-21T05:49:32Z","timestamp":1666331372000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":32,"title":["GluA2\u2010lacking AMPA receptors in the nucleus accumbens core and shell contribute to the incubation of oxycodone craving in male rats"],"prefix":"10.1111","volume":"27","author":[{"given":"Benjamin","family":"Wong","sequence":"first","affiliation":[{"name":"Department of Psychology and Neuroscience North Central College  Naperville Illinois USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexa R.","family":"Zimbelman","sequence":"additional","affiliation":[{"name":"Department of Psychology and Neuroscience North Central College  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