{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,24]],"date-time":"2025-10-24T16:48:00Z","timestamp":1761324480681,"version":"3.41.2"},"reference-count":70,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2022,10,12]],"date-time":"2022-10-12T00:00:00Z","timestamp":1665532800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000027","name":"National Institute on Alcohol Abuse and Alcoholism","doi-asserted-by":"publisher","award":["F32AA027702","R01AA024798","R01AA027653"],"award-info":[{"award-number":["F32AA027702","R01AA024798","R01AA027653"]}],"id":[{"id":"10.13039\/100000027","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Addiction Biology"],"published-print":{"date-parts":[[2022,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Embryonic exposure to ethanol increases the risk for alcohol use disorder in humans and stimulates alcohol\u2010related behaviours in different animal models. Evidence in rats and zebrafish suggests that this phenomenon induced by ethanol at low\u2010moderate concentrations involves a stimulatory effect on neurogenesis and density of hypothalamic neurons expressing the peptides, hypocretin\/orexin (Hcrt) and melanin\u2010concentrating hormone (MCH), known to promote alcohol consumption. Building on our report in zebrafish showing that ethanol induces ectopic expression of Hcrt neurons outside the hypothalamus, we investigated here whether embryonic ethanol exposure also induces ectopic peptide neurons in rats similar to zebrafish and affects their morphological characteristics and if these ectopic neurons are functional and have a role in the ethanol\u2010induced disturbances in behaviour. We demonstrate in rats that ethanol at a low\u2010moderate dose, in addition to increasing Hcrt and MCH neurons in the lateral hypothalamus where they are normally concentrated, induces ectopic expression of these peptide neurons further anterior in the nucleus accumbens core and ventromedial caudate putamen where they have not been previously observed and causes morphological changes relative to normally located hypothalamic neurons. Similar to rats, embryonic ethanol exposure at a low\u2010moderate dose in zebrafish induces ectopic Hcrt neurons anterior to the hypothalamus and alters their morphology. Notably, laser ablation of these ectopic Hcrt neurons blocks the behavioural effects induced by ethanol exposure, including increased anxiety and locomotor activity. These findings suggest that the ectopic peptide neurons are functional and contribute to the ethanol\u2010induced behavioural disturbances related to the overconsumption of alcohol.<\/jats:p>","DOI":"10.1111\/adb.13238","type":"journal-article","created":{"date-parts":[[2022,10,12]],"date-time":"2022-10-12T09:25:17Z","timestamp":1665566717000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Embryonic ethanol exposure induces ectopic Hcrt and MCH neurons outside hypothalamus in rats and zebrafish: Role in ethanol\u2010induced behavioural disturbances"],"prefix":"10.1111","volume":"27","author":[{"given":"Adam D.","family":"Collier","sequence":"first","affiliation":[{"name":"Laboratory of Behavioral Neurobiology The Rockefeller University  New York New York USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nushrat","family":"Yasmin","sequence":"additional","affiliation":[{"name":"Laboratory of Behavioral Neurobiology The Rockefeller University  New York New York USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guo\u2010Qing","family":"Chang","sequence":"additional","affiliation":[{"name":"Laboratory of Behavioral Neurobiology The Rockefeller University  New York New York USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Olga","family":"Karatayev","sequence":"additional","affiliation":[{"name":"Laboratory of Behavioral Neurobiology The Rockefeller University  New York New York USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nailya","family":"Khalizova","sequence":"additional","affiliation":[{"name":"Laboratory of Behavioral Neurobiology The Rockefeller University  New York New York USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Milisia","family":"Fam","sequence":"additional","affiliation":[{"name":"Laboratory of Behavioral Neurobiology The Rockefeller University  New York New York USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abdul R.","family":"Abdulai","sequence":"additional","affiliation":[{"name":"Laboratory of Behavioral Neurobiology The Rockefeller University  New York New York USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Boyi","family":"Yu","sequence":"additional","affiliation":[{"name":"Laboratory of Behavioral Neurobiology The Rockefeller University  New York New York USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8886-4182","authenticated-orcid":false,"given":"Sarah F.","family":"Leibowitz","sequence":"additional","affiliation":[{"name":"Laboratory of Behavioral Neurobiology The Rockefeller University  New York New York USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,10,12]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/acer.13135"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.0637\u201018.2018"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010021\u201095707\u2010y"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0361\u20109230(85)90213\u20108"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.1.322"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainres.2020.146872"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978\u20100\u2010444\u201059489\u20101.00009\u20104"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/7854_2016_57"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2012.05.066"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbr.2016.01.013"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/acer.14126"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/7854_2016_59"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2021.721924"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1089\/zeb.2016.1270"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2015.09.020"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnana.2017.00110"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainres.2021.147587"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978\u20100\u201012\u2010394310\u20101.00007\u20102"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.5110\u201007.2008"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.21149"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/s000180050367"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1474\u20104422(19)30150\u20104"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1528\u20101167.2012.03466.x"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jneumeth.2008.03.002"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-814338-4.00006-4"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pnpbp.2022.110536"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2019.10.013"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.906637106"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.5500\u201011.2012"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquatox.2017.08.005"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2008.09.025"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2014.10.010"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2019.08.060"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2019.10.013"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroimage.2010.09.025"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.3389\/fncir.2015.00078"},{"volume-title":"Stereotaxic Atlas of the Developing Rat Brain","year":"1970","author":"Sherwood NM","key":"e_1_2_9_38_1"},{"key":"e_1_2_9_39_1","article-title":"Ablation of a neuronal population using a two\u2010photon laser and its assessment using calcium imaging and behavioral recording in zebrafish larvae","volume":"136","author":"Muto A","year":"2018","journal-title":"J vis Exp"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.3791\/60966"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1002\/jat.4171"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbr.2011.12.016"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-61779-597-8_2"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbr.2014.09.024"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.32607\/20758251\u20102015\u20107\u20102\u201042\u201047"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1111\/acer.14296"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1111\/acer.14482"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11481-016-9691-8"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.physbeh.2009.07.012"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1530\u20100277.2011.01520.x"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1530\u20100277.2012.01811.x"},{"key":"e_1_2_9_52_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.alcohol.2011.11.008"},{"key":"e_1_2_9_53_1","doi-asserted-by":"publisher","DOI":"10.1176\/appi.ajp.2020.20010086"},{"key":"e_1_2_9_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neubiorev.2006.06.021"},{"key":"e_1_2_9_55_1","doi-asserted-by":"publisher","DOI":"10.1080\/10298420290030532"},{"key":"e_1_2_9_56_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2021.08.028"},{"key":"e_1_2_9_57_1","doi-asserted-by":"publisher","DOI":"10.1038\/npp.2011.140"},{"key":"e_1_2_9_58_1","doi-asserted-by":"publisher","DOI":"10.1111\/adb.12946"},{"key":"e_1_2_9_59_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopsych.2018.07.022"},{"key":"e_1_2_9_60_1","doi-asserted-by":"publisher","DOI":"10.1111\/adb.12795"},{"key":"e_1_2_9_61_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0053991"},{"key":"e_1_2_9_62_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0069574"},{"key":"e_1_2_9_63_1","doi-asserted-by":"publisher","DOI":"10.1002\/bdr2.1477"},{"key":"e_1_2_9_64_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnana.2015.00002"},{"key":"e_1_2_9_65_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbr.2005.08.020"},{"key":"e_1_2_9_66_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1530\u20100277.2011.01668.x"},{"key":"e_1_2_9_67_1","doi-asserted-by":"publisher","DOI":"10.1111\/acer.13350"},{"key":"e_1_2_9_68_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.alcohol.2010.05.002"},{"key":"e_1_2_9_69_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.physbeh.2013.10.005"},{"key":"e_1_2_9_70_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.07000"},{"key":"e_1_2_9_71_1","doi-asserted-by":"publisher","DOI":"10.1159\/000514964"}],"container-title":["Addiction Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/adb.13238","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/adb.13238","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/adb.13238","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,21]],"date-time":"2023-08-21T06:23:38Z","timestamp":1692599018000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/adb.13238"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,10,12]]},"references-count":70,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2022,11]]}},"alternative-id":["10.1111\/adb.13238"],"URL":"https:\/\/doi.org\/10.1111\/adb.13238","archive":["Portico"],"relation":{},"ISSN":["1355-6215","1369-1600"],"issn-type":[{"type":"print","value":"1355-6215"},{"type":"electronic","value":"1369-1600"}],"subject":[],"published":{"date-parts":[[2022,10,12]]},"assertion":[{"value":"2022-05-03","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-09-22","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-10-12","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e13238"}}