{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,18]],"date-time":"2026-01-18T14:21:36Z","timestamp":1768746096309,"version":"3.49.0"},"reference-count":59,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2024,2,6]],"date-time":"2024-02-06T00:00:00Z","timestamp":1707177600000},"content-version":"vor","delay-in-days":5,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81971785"],"award-info":[{"award-number":["81971785"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003787","name":"Natural Science Foundation of Hebei Province","doi-asserted-by":"publisher","award":["H2022206026"],"award-info":[{"award-number":["H2022206026"]}],"id":[{"id":"10.13039\/501100003787","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Addiction Biology"],"published-print":{"date-parts":[[2024,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Recent studies found that non\u2010coding RNAs (ncRNAs) played crucial roles in drug addiction through epigenetic regulation of gene expression and underlying drug\u2010induced neuroadaptations. In this study, we characterized lncRNA transcriptome profiles in the nucleus accumbens (NAc) of mice exhibiting morphine\u2010conditioned place preference (CPP) and explored the prospective roles of novel differentially expressed lncRNA, lncLingo2 and its derived miR\u2010876\u20105p in the acquisition of opioids\u2010associated behaviours. We found that the lncLingo2 was downregulated within the NAc core (NAcC) but not in the NAc shell (NAcS). This downregulation was found to be associated with the development of morphine CPP and heroin intravenous self\u2010administration (IVSA). As Mfold software revealed that the secondary structures of lncLingo2 contained the sequence of pre\u2010miR\u2010876, transfection of LV\u2010lncLingo2 into HEK293 cells significantly upregulated miR\u2010876 expression and the changes of mature miR\u2010876 are positively correlated with lncLingo2 expression in NAcC of morphine CPP trained mice. Delivering miR\u2010876\u20105p mimics into NAcC also inhibited the acquisition of morphine CPP. Furthermore, bioinformatics analysis and dual\u2010luciferase assay confirmed that miR\u2010876\u20105p binds to its target gene, <jats:italic>Kcnn3<\/jats:italic>, selectively and regulates morphine CPP training\u2010induced alteration of <jats:italic>Kcnn3<\/jats:italic> expression. Lastly, the electrophysiological analysis indicated that the currents of small conductance calcium\u2010activated potassium (SK) channel was increased, which led to low neuronal excitability in NAcC after CPP training, and these changes were reversed by lncLingo2 overexpression. Collectively, lncLingo2 may function as a precursor of miR\u2010876\u20105p in NAcC, hence modulating the development of opioid\u2010associated behaviours in mice, which may serve as an underlying biomarker and therapeutic target of opioid addiction.<\/jats:p>","DOI":"10.1111\/adb.13375","type":"journal-article","created":{"date-parts":[[2024,2,7]],"date-time":"2024-02-07T06:48:53Z","timestamp":1707288533000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Roles of lncLingo2 and its derived miR\u2010876\u20105p in the acquisition of opioid reinforcement"],"prefix":"10.1111","volume":"29","author":[{"given":"Hongyu","family":"Yang","sequence":"first","affiliation":[{"name":"College of Forensic Medicine, Hebei Medical University, Hebei Key Laboratory of Forensic Medicine, Collaborative Innovation Center of Forensic Medical Molecular Identification, Research Unit of Digestive Tract Microecosystem Pharmacology and Toxicology Chinese Academy of Medical Sciences  Shijiazhuang Hebei Province China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiuning","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Forensic Medicine, Hebei Medical University, Hebei Key Laboratory of Forensic Medicine, Collaborative Innovation Center of Forensic Medical Molecular Identification, Research Unit of Digestive Tract Microecosystem Pharmacology and Toxicology Chinese Academy of Medical Sciences  Shijiazhuang Hebei Province China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-6627-0774","authenticated-orcid":false,"given":"Minglong","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Forensic Medicine, Hebei Medical University, Hebei Key Laboratory of Forensic Medicine, Collaborative Innovation Center of Forensic Medical Molecular Identification, Research Unit of Digestive Tract Microecosystem Pharmacology and Toxicology Chinese Academy of Medical Sciences  Shijiazhuang Hebei Province China"},{"name":"Department of Genetics Qiqihar Medical University  Qiqihar Heilongjiang Province China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yun","family":"Lu","sequence":"additional","affiliation":[{"name":"College of Forensic Medicine, Hebei Medical University, Hebei Key Laboratory of Forensic Medicine, Collaborative Innovation Center of Forensic Medical Molecular Identification, Research Unit of Digestive Tract Microecosystem Pharmacology and Toxicology Chinese Academy of Medical Sciences  Shijiazhuang Hebei Province China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bing","family":"Xie","sequence":"additional","affiliation":[{"name":"College of Forensic Medicine, Hebei Medical University, Hebei Key Laboratory of Forensic Medicine, Collaborative Innovation Center of Forensic Medical Molecular Identification, Research Unit of Digestive Tract Microecosystem Pharmacology and Toxicology Chinese Academy of Medical Sciences  Shijiazhuang Hebei Province China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shaoguang","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, Key Laboratory of Medical Biotechnology of Hebei Province Hebei Medical University  Shijiazhuang China"},{"name":"Key Laboratory of Neural and Vascular Biology Ministry of Education  Shijiazhuang Hebei Province China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hailei","family":"Yu","sequence":"additional","affiliation":[{"name":"College of Forensic Medicine, Hebei Medical University, Hebei Key Laboratory of Forensic Medicine, Collaborative Innovation Center of Forensic Medical Molecular Identification, Research Unit of Digestive Tract Microecosystem Pharmacology and Toxicology Chinese Academy of Medical Sciences  Shijiazhuang Hebei Province China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3325-371X","authenticated-orcid":false,"given":"Bin","family":"Cong","sequence":"additional","affiliation":[{"name":"College of Forensic Medicine, Hebei Medical University, Hebei Key Laboratory of Forensic Medicine, Collaborative Innovation Center of Forensic Medical Molecular Identification, Research Unit of Digestive Tract Microecosystem Pharmacology and Toxicology Chinese Academy of Medical Sciences  Shijiazhuang Hebei Province China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yixiao","family":"Luo","sequence":"additional","affiliation":[{"name":"Hunan Province People's Hospital The First\u2010Affiliated Hospital of Hunan Normal University  Changsha China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chunling","family":"Ma","sequence":"additional","affiliation":[{"name":"College of Forensic Medicine, Hebei Medical University, Hebei Key Laboratory of Forensic Medicine, Collaborative Innovation Center of Forensic Medical Molecular Identification, Research Unit of Digestive Tract Microecosystem Pharmacology and Toxicology Chinese Academy of Medical Sciences  Shijiazhuang Hebei Province China"},{"name":"Key Laboratory of Neural and Vascular Biology Ministry of Education  Shijiazhuang Hebei Province China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Di","family":"Wen","sequence":"additional","affiliation":[{"name":"College of Forensic Medicine, Hebei Medical University, Hebei Key Laboratory of Forensic Medicine, Collaborative Innovation Center of Forensic Medical Molecular Identification, Research Unit of Digestive Tract Microecosystem Pharmacology and Toxicology Chinese Academy of Medical Sciences  Shijiazhuang Hebei Province China"},{"name":"Key Laboratory of Neural and Vascular Biology Ministry of Education  Shijiazhuang Hebei Province China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,2,6]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1196\/annals.1441.020"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1385\/1\u201059259\u2010358\u20105:75"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1215070"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1101\/lm.78504"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/ejn.14072"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.coph.2020.08.007"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0163\u20101047(79)92959\u20105"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.0703\u201017.2017"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nlm.2013.06.017"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1006\/nlme.2002.4103"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/adb.12273"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1093\/ijnp\/pyv030"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1037\/a0036964"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.7150\/thno.34655"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.2829\u201020.2021"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.1918\u201020.2021"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuropharm.2013.04.004"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.3389\/fgene.2012.00106"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41398\u2010019\u20100527\u20101"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.3389\/fgene.2014.00164"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/jnc.12006"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12868\u2010015\u20100157\u20103"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpsyt.2022.915398"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1080\/13880209.2019.1651343"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopsych.2019.06.027"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41380\u2010022\u201001459\u20100"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpsychires.2023.05.057"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1523\/jneurosci.2873\u201016.2017"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41386\u2010019\u20100415\u20107"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.17446611"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.neuro.29.051605.112839"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm1784"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00036.2006"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pnpbp.2022.110515"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells11121949"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41380\u2010022\u201001651\u20102"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1093\/ijnp\/pyac004"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phrs.2021.105722"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1111\/jnc.13255"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuropharm.2004.07.005"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1038\/nn.2367"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2014.08.023"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1038\/nn1217"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0904715106"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1007\/978\u20101\u20104614\u20100332\u20106_12"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1231776"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1038\/nsmb.2480"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mcn.2004.03.002"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.1006\/mcne.2000.0842"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.2174\/0929867033457908"},{"key":"e_1_2_9_52_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1460\u20109568.2003.02488.x"},{"key":"e_1_2_9_53_1","doi-asserted-by":"publisher","DOI":"10.1038\/nn1101"},{"key":"e_1_2_9_54_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1601\u2010183X.2008.00416.x"},{"key":"e_1_2_9_55_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuropharm.2010.10.024"},{"key":"e_1_2_9_56_1","doi-asserted-by":"publisher","DOI":"10.7150\/thno.44893"},{"key":"e_1_2_9_57_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnmol.2018.00258"},{"key":"e_1_2_9_58_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.0105\u201019.2019"},{"key":"e_1_2_9_59_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopsych.2021.10.004"},{"key":"e_1_2_9_60_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41380\u2010020\u20100816\u20103"}],"container-title":["Addiction Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/adb.13375","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,12,5]],"date-time":"2024-12-05T08:14:47Z","timestamp":1733386487000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/adb.13375"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,2]]},"references-count":59,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2024,2]]}},"alternative-id":["10.1111\/adb.13375"],"URL":"https:\/\/doi.org\/10.1111\/adb.13375","archive":["Portico"],"relation":{},"ISSN":["1355-6215","1369-1600"],"issn-type":[{"value":"1355-6215","type":"print"},{"value":"1369-1600","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,2]]},"assertion":[{"value":"2023-11-14","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-01-15","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-02-06","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e13375"}}