{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T00:45:57Z","timestamp":1760057157698,"version":"build-2065373602"},"reference-count":54,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2025,7,29]],"date-time":"2025-07-29T00:00:00Z","timestamp":1753747200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Addiction Biology"],"published-print":{"date-parts":[[2025,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Alcohol use disorder is closely related to genetic and environmental factors. However, the contribution of coding variation to alcohol use disorder susceptibility remains poorly understood. We aimed to identify genetic mutations in alcohol use disorder by whole exon sequencing. We performed whole\u2010exome sequencing in 83 patients with alcohol use disorder and compared it with exome sequences of healthy controls that were collected from the 1000 Genomes Project. GO and KEGG enrichment analysis and protein interaction analysis were performed for the mutated genes in each group. Three online protein function prediction sites were used to predict whether SNPs\/InDels cause protein coding changes. Further, we conducted a rare variant exploration. We identified 106\u2009525 SNV and 19\u2009826 InDel gene mutations in alcohol use disorder. In the healthy and alcohol use disorder groups, mutations in CNTNAP3, ZNF683, ALDPH2, CCHCR1, ZNF45 and ESRRA loci were found to be deleterious mutations in all three sites; CNTNAP3, ZNF683, ALDPH2, CCHCR1, ZNF45 and ESRRA may be potential targets for future precision treatment of alcohol use disorders, and further provide new ideas for drug development.<\/jats:p>","DOI":"10.1111\/adb.70070","type":"journal-article","created":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T02:05:04Z","timestamp":1753841104000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Genome Variation in Alcohol Use Disorder by Whole\u2010Exome Sequencing"],"prefix":"10.1111","volume":"30","author":[{"given":"Lei","family":"Liu","sequence":"first","affiliation":[{"name":"Department of Psychiatry The First Affiliated Hospital of Harbin Medical University  Harbin Heilongjiang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3838-4025","authenticated-orcid":false,"given":"Bo","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Psychology Shandong Provincial Hospital Affiliated to Shandong First Medical University  Jinan Shandong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yong","family":"Dong","sequence":"additional","affiliation":[{"name":"Department of Psychiatry Changchun Sixth Hospital  Changchun China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Li\u00a0Ping","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Psychiatry The First Special Hospital of Harbin  Harbin Heilongjiang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jing\u00a0Ying","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Psychiatry Changchun Sixth Hospital  Changchun China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jun","family":"Liu","sequence":"additional","affiliation":[{"name":"The Third Hospital of Heilongjiang Province  Harbin Heilongjiang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guang\u00a0Yu","family":"Zhou","sequence":"additional","affiliation":[{"name":"Shenyang Mental Health Center  Shenyang Liaoning China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chuan\u00a0Yi","family":"Kang","sequence":"additional","affiliation":[{"name":"Department of Psychiatry The First Affiliated Hospital of Harbin Medical University  Harbin Heilongjiang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaorui","family":"Hu","sequence":"additional","affiliation":[{"name":"Department of Psychiatry The First Affiliated Hospital of Harbin Medical University  Harbin Heilongjiang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chang","family":"Cheng","sequence":"additional","affiliation":[{"name":"Department of Psychiatry The First Affiliated Hospital of Harbin Medical University  Harbin Heilongjiang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Na","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Psychiatry The First Affiliated Hospital of Harbin Medical University  Harbin Heilongjiang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jia","family":"Lu","sequence":"additional","affiliation":[{"name":"Department of Psychiatry The First Affiliated Hospital of Harbin Medical University  Harbin Heilongjiang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huaizhi","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Psychiatry The First Affiliated Hospital of Harbin Medical University  Harbin Heilongjiang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jian","family":"Hu","sequence":"additional","affiliation":[{"name":"Department of Psychiatry The First Affiliated Hospital of Harbin Medical University  Harbin Heilongjiang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaohong","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Psychiatry The First Affiliated Hospital of Harbin Medical University  Harbin Heilongjiang China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,7,29]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuropharm"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1097\/YPG.0000000000000169"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.drugalcdep"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.5114\/amsik.2016.66401"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/acer.12792"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010019\u201011916\u20100"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1086\/383283"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/mp.2013.145"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00017.2013"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1530\u20100277.2012.01916.x"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41593\u2010018\u20100275\u20101"},{"issue":"6","key":"e_1_2_11_13_1","first-page":"391","article-title":"The Limitations of GWAS in Understanding the Genetic Basis of Complex Diseases","volume":"10","author":"Sullivan P. F.","year":"2009","journal-title":"Nature Reviews Genetics"},{"issue":"2","key":"e_1_2_11_14_1","first-page":"119","article-title":"Genome\u2010Wide Association Studies and Rare Variants in Psychiatric Genetics","volume":"29","author":"Neale B.","year":"2016","journal-title":"Current Opinion in Psychiatry"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjh.16249"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/978\u20101\u20104939\u20107471\u20109_9"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1136\/jmedgenet\u20102011\u2010100223"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/gim.2017.247"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.4306\/pi.2016.13.3.255"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/pcn.1277430293238"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/epi.16755"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586\u2010022\u201004556\u2010w"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41588\u2010022\u201001148\u20102"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpsyt.2023.121649337915799"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.3390\/jcm1013281034202351"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/acer.13614"},{"issue":"1","key":"e_1_2_11_27_1","doi-asserted-by":"crossref","first-page":"59","DOI":"10.35946\/arcr.v38.1.08","article-title":"Biology, Genetics, and Environment: Underlying Factors Influencing Alcohol Metabolism","volume":"38","author":"Wall T. L.","year":"2016","journal-title":"Alcohol Research: Current Reviews"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopsych.2025.01.020"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.31557\/APJCP.2023.24.6.2073"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.23670"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nbd.2019.104486"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gene.2012.09.006"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0147887"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.1590\/1806\u20109282.61.04.381"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2017.00535"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.1002\/ctm2.1065"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12864\u2010018\u20104810\u2010y"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexcr.2014.05.008"},{"key":"e_1_2_11_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexcr.2021.112471"},{"key":"e_1_2_11_40_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0006030"},{"key":"e_1_2_11_41_1","doi-asserted-by":"publisher","DOI":"10.3390\/biomedicines9070840"},{"key":"e_1_2_11_42_1","doi-asserted-by":"publisher","DOI":"10.4103\/idoj.IDOJ_226_20"},{"key":"e_1_2_11_43_1","doi-asserted-by":"publisher","DOI":"10.2147\/PTT.S164104"},{"key":"e_1_2_11_44_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00438\u2010020\u201001678\u20106"},{"key":"e_1_2_11_45_1","doi-asserted-by":"publisher","DOI":"10.1002\/ajmg.b.32750"},{"key":"e_1_2_11_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nbd.2023.106209"},{"key":"e_1_2_11_47_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010022\u201006010\u20103"},{"key":"e_1_2_11_48_1","doi-asserted-by":"publisher","DOI":"10.3233\/JAD\u2010230181"},{"key":"e_1_2_11_49_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrg2538"},{"key":"e_1_2_11_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tem.2008.07.005"},{"key":"e_1_2_11_51_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21051645"},{"key":"e_1_2_11_52_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mito.2008.02.001"},{"key":"e_1_2_11_53_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0006\u20103223(01)01289\u20106"},{"key":"e_1_2_11_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neulet.2008.01.076"},{"key":"e_1_2_11_55_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pnpbp.2019.01.008"}],"container-title":["Addiction Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/adb.70070","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T15:18:07Z","timestamp":1760023087000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/adb.70070"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,7,29]]},"references-count":54,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2025,8]]}},"alternative-id":["10.1111\/adb.70070"],"URL":"https:\/\/doi.org\/10.1111\/adb.70070","archive":["Portico"],"relation":{},"ISSN":["1355-6215","1369-1600"],"issn-type":[{"type":"print","value":"1355-6215"},{"type":"electronic","value":"1369-1600"}],"subject":[],"published":{"date-parts":[[2025,7,29]]},"assertion":[{"value":"2024-10-05","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-07-09","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-07-29","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70070"}}