{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T02:02:00Z","timestamp":1784685720156,"version":"3.55.0"},"reference-count":51,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T00:00:00Z","timestamp":1777334400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T00:00:00Z","timestamp":1777334400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2013\/01158\u20107"],"award-info":[{"award-number":["2013\/01158\u20107"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2014\/27198\u20108"],"award-info":[{"award-number":["2014\/27198\u20108"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2021\/13092\u20107"],"award-info":[{"award-number":["2021\/13092\u20107"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2023\/13554\u20106"],"award-info":[{"award-number":["2023\/13554\u20106"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Addiction Biology"],"published-print":{"date-parts":[[2026,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Alcohol use disorder (AUD) is a chronic and progressive disease that affects individuals worldwide. AUD individuals exhibit epigenetic alterations across tissues, mainly in the brain. Despite advances in the field, there is a gap in epigenome\u2010wide association studies (EWAS) conducted in a Brazilian cohort that is genetically and culturally diverse. This study aimed to describe an exploratory EWAS conducted in Brazilian males with AUD and to identify DNA methylation signatures associated with brain\u2010related biological pathways. Peripheral blood samples were collected from 54 patients with AUD in the early stages of withdrawal (AUD group) and 70 control individuals (CON group) (Brazil, S\u00e3o Paulo). Genome\u2010wide DNA methylation was assessed using the Infinium MethylationEPIC BeadChip v2.0. We identified four differentially methylated loci in genes involved in intracellular signalling and cellular stress responses. Focusing on the nervous system, enrichment analyses revealed pathways related to neuronal\/axon development and synaptic organization hypomethylated in the AUD group (\n                    <jats:italic>p<\/jats:italic>\n                    . adjust &lt;\u20090.05). Differentially methylated regions (DMRs) showed hypomethylation in regulatory and exonic regions of\n                    <jats:italic>ABAT, DLX5, PHGDH, TRPM2<\/jats:italic>\n                    and\n                    <jats:italic>GABBR1<\/jats:italic>\n                    genes, which are within the enriched pathways. In conclusion, to the best of our knowledge, this is the first EWAS in a Brazilian cohort with AUD to identify alterations in DNA methylation, highlighting genes and pathways involved in neurogenesis, synaptic and GABAergic signalling. These epigenetic modifications suggest an impact of chronic alcohol consumption on critical biological processes of the CNS, potentially contributing to the cognitive and behavioural impairments observed in AUD. The results reinforce the importance of epigenetic studies in mixed\u2010race populations.\n                  <\/jats:p>","DOI":"10.1111\/adb.70161","type":"journal-article","created":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T10:18:00Z","timestamp":1777371480000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Blood\u2010Based Epigenetic Signatures in Brazilian Males With Alcohol Use Disorder"],"prefix":"10.1111","volume":"31","author":[{"given":"La\u00eds","family":"da Silva Pereira\u2010Rufino","sequence":"first","affiliation":[{"name":"Departamento de Morfologia e Gen\u00e9tica, Escola Paulista de Medicina Universidade Federal de S\u00e3o Paulo \u2013 UNIFESP  S\u00e3o Paulo SP Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raissa Mazzer","family":"de Sino Romano","sequence":"additional","affiliation":[{"name":"Departamento de Morfologia e Gen\u00e9tica, Escola Paulista de Medicina Universidade Federal de S\u00e3o Paulo \u2013 UNIFESP  S\u00e3o Paulo SP Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Regiane Chiavelli","family":"Lamim","sequence":"additional","affiliation":[{"name":"Departamento de Oncologia Cl\u00ednica e Experimental, Escola Paulista de Medicina Universidade Federal de S\u00e3o Paulo \u2013 UNIFESP  S\u00e3o Paulo SP Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rafael","family":"Conte","sequence":"additional","affiliation":[{"name":"Departamento de Morfologia e Gen\u00e9tica, Escola Paulista de Medicina Universidade Federal de S\u00e3o Paulo \u2013 UNIFESP  S\u00e3o Paulo SP Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Denise Ribeiro","family":"Gobbo","sequence":"additional","affiliation":[{"name":"Departamento de Morfologia e Gen\u00e9tica, Escola Paulista de Medicina Universidade Federal de S\u00e3o Paulo \u2013 UNIFESP  S\u00e3o Paulo SP Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcelo Carvalho","family":"da Concei\u00e7\u00e3o","sequence":"additional","affiliation":[{"name":"Departamento de Morfologia e Gen\u00e9tica, Escola Paulista de Medicina Universidade Federal de S\u00e3o Paulo \u2013 UNIFESP  S\u00e3o Paulo SP Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vanessa Kiyomi","family":"Ota","sequence":"additional","affiliation":[{"name":"Departamento de Morfologia e Gen\u00e9tica, Escola Paulista de Medicina Universidade Federal de S\u00e3o Paulo \u2013 UNIFESP  S\u00e3o Paulo SP Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucas","family":"Liro","sequence":"additional","affiliation":[{"name":"Departamento de Patologia, Faculdade de Medicina Universidade de S\u00e3o Paulo \u2013 USP  S\u00e3o Paulo SP Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leslie Domenici","family":"Kulikowski","sequence":"additional","affiliation":[{"name":"Departamento de Patologia, Faculdade de Medicina Universidade de S\u00e3o Paulo \u2013 USP  S\u00e3o Paulo SP Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adriana Marcassa","family":"Tucci","sequence":"additional","affiliation":[{"name":"Departamento de Bioci\u00eancias Universidade Federal de S\u00e3o Paulo \u2013 UNIFESP  S\u00e3o Paulo SP Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5643-3321","authenticated-orcid":false,"given":"Ana Lucia","family":"de Moraes Horta","sequence":"additional","affiliation":[{"name":"Departamento de Sa\u00fade Coletiva Universidade Federal de S\u00e3o Paulo \u2013 UNIFESP  S\u00e3o Paulo SP Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana Maria","family":"Sebasti\u00e3o","sequence":"additional","affiliation":[{"name":"Instituto de Farmacologia e Neuroci\u00eancias, Faculdade de Medicina Universidade de Lisboa  Lisbon Portugal"},{"name":"Centro Cardiovascular da Universidade de Lisboa, CCUL, Faculdade de Medicina Universidade de Lisboa  Lisbon Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Lucia Oliveira","family":"Souza\u2010Formigoni","sequence":"additional","affiliation":[{"name":"Departamento de Psicobiologia, Escola Paulista de Medicina Universidade Federal de S\u00e3o Paulo \u2013 UNIFESP  S\u00e3o Paulo SP Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jo\u00e3o Ricardo Nickenig","family":"Vissoci","sequence":"additional","affiliation":[{"name":"Duke Global Health Institute Duke University  Durham North Carolina USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandre Ferro","family":"Aissa","sequence":"additional","affiliation":[{"name":"Departamento de Morfologia e Gen\u00e9tica, Escola Paulista de Medicina Universidade Federal de S\u00e3o Paulo \u2013 UNIFESP  S\u00e3o Paulo SP Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4806-3548","authenticated-orcid":false,"given":"Isabel Cristina","family":"C\u00e9spedes","sequence":"additional","affiliation":[{"name":"Departamento de Morfologia e Gen\u00e9tica, Escola Paulista de Medicina Universidade Federal de S\u00e3o Paulo \u2013 UNIFESP  S\u00e3o Paulo SP Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,4,28]]},"reference":[{"key":"e_1_2_9_2_1","unstructured":"\u201cGlobal Status Report on Alcohol and Health and Treatment of Substance Use Disorders.\u201d [Internet]. Geneva; (2024) [cited 2024 Oct 10] https:\/\/www.who.int\/publications\/i\/item\/9789240096745."},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/gbb.12864"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuint.2023.105627"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI172885"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.2174\/1871527320666210825101915"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra1402513"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.2174\/0109298673256937231004093143"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/adb.13006"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.3390\/brainsci14060554"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1037\/neu0000575"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13148-025-01893-1"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.2217\/epi-2022-0055"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41380-020-0734-4"},{"key":"e_1_2_9_15_1","article-title":"Admixture's Impact on Brazilian Population Evolution and Health","volume":"388","author":"Nunes K.","year":"2025","journal-title":"Science (1979)"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12864-025-11691-x"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-022-12127-2"},{"key":"e_1_2_9_18_1","unstructured":"R.Humeniuk S.Henry\u2010Edwards R.Ali V.Poznyak andM. G.Monteiro \u201cThe Alcohol Smoking and Substance Involvement Screening Test (ASSIST): Manual for Use in Primary Care \u201d (2010)."},{"key":"e_1_2_9_19_1","unstructured":"R Core Team \u201cA Language and Environment for Statistical Computing \u201d 8 https:\/\/www.r\u2010project.org\/. Vienna Austria: Open Access Library Journal 2020 (2020)."},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0041361"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkt090"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-11-587"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkv007"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2632"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1186\/gb-2014-15-2-r31"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1186\/1756-8935-8-6"},{"issue":"09","key":"e_1_2_9_27_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.4236\/oalib.1107821","article-title":"The Exploration of the Approach to Data Preparation for Chinese Text Analysis Based on R Language","volume":"08","author":"Li J.","year":"2021","journal-title":"OAlib"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41596-018-0103-9"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12859-017-1571-6"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btr671"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41596-024-01020-z"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btr260"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1093\/aje\/kwz032"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41380-021-01378-6"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13148-025-01818-y"},{"key":"e_1_2_9_36_1","doi-asserted-by":"crossref","unstructured":"L.Zillich S.D'Augello D.Avetyan et\u00a0al. \u201cDNA Methylation Signatures of Alcohol Use Disorder \u2013 A Large\u2010Scale Meta\u2010Analysis in the Psychiatric Genomics Consortium \u201d (2025).","DOI":"10.1101\/2025.08.29.25334705"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms241210130"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.3389\/fgene.2024.1345410"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymgme.2025.109149"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2017.10.032"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.3389\/fncir.2023.1218737"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2215-0366(16)00104-8"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.202684"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells11111739"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pneurobio.2020.101896"},{"key":"e_1_2_9_46_1","first-page":"401","article-title":"Phosphoglycerate Dehydrogenase and Alzheimer's Disease","volume":"24","author":"Liu Q. Y.","year":"2025","journal-title":"EXCLI Journal"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2023.1217828"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1111\/acer.70180"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.1111\/add.15020"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.1111\/adb.12855"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.1002\/ajmg.b.32833"},{"key":"e_1_2_9_52_1","doi-asserted-by":"publisher","DOI":"10.1111\/acer.14354"}],"container-title":["Addiction Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/adb.70161","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/adb.70161","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/adb.70161","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T01:18:08Z","timestamp":1784683088000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/adb.70161"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,4,28]]},"references-count":51,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2026,5]]}},"alternative-id":["10.1111\/adb.70161"],"URL":"https:\/\/doi.org\/10.1111\/adb.70161","archive":["Portico"],"relation":{},"ISSN":["1355-6215","1369-1600"],"issn-type":[{"value":"1355-6215","type":"print"},{"value":"1369-1600","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,4,28]]},"assertion":[{"value":"2025-11-13","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-04-16","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-04-28","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70161"}}