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Therefore, to contribute to ending the controversy, this study aimed to detect and compare methanogens' presence in two distinct niches with supposedly different microenvironments; both were necrotic root canals associated with AP but one from patients with type 2 diabetes mellitus (T2DM) while the other from non\u2010diabetic patients. A clinical examination was performed on 65 T2DM patients and 73 non\u2010diabetic controls. Samples from necrotic root canals were obtained, and methanogens were identified. The presence of methanogens was three times higher (27.6%) in the T2DM group than in non\u2010diabetic patients (8.2%). In addition, methanogens' presence was associated with a higher prevalence of periapical symptoms.<\/jats:p>","DOI":"10.1111\/aej.12797","type":"journal-article","created":{"date-parts":[[2023,9,16]],"date-time":"2023-09-16T05:16:05Z","timestamp":1694841365000},"page":"641-647","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Presence of methanogenic archaea in necrotic root canals of patients with or without type 2 diabetes mellitus"],"prefix":"10.1111","volume":"49","author":[{"given":"Ana I.","family":"Soza\u2010Bola\u00f1os","sequence":"first","affiliation":[{"name":"Laboratory of Multidisciplinary Dentistry Research, Facultad de Medicina Universidad Aut\u00f3noma de Quer\u00e9taro  Santiago de Quer\u00e9taro Mexico"},{"name":"Department of Endodontics, Facultad de Medicina Universidad Aut\u00f3noma de Quer\u00e9taro  Santiago de Quer\u00e9taro 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