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Sixty freshly extracted premolars were stored in formalin, water, or dry conditions (<jats:italic>n<\/jats:italic>\u2009=\u200920) and scanned after 72\u2009h, 30\u2009days, 6\u2009months and 3\u2009years of extraction. The effect of the storage medium and time on the occurrence of dentinal defects was statistically evaluated. A total of 211\u2009000 images were screened revealing the existence of 11\u2009519 slices with dentinal defects. Dry conditions significantly contributed to the development of new defects in all time points. During the 3\u2010year follow\u2010up period, no new defects were detected in the teeth that were stored in water and, in a single tooth, in the formalin group, after 6\u2009months of storage.<\/jats:p>","DOI":"10.1111\/aej.12806","type":"journal-article","created":{"date-parts":[[2023,10,25]],"date-time":"2023-10-25T06:58:45Z","timestamp":1698217125000},"page":"60-68","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["The impact of long\u2010term storage conditions on the development of experimental dentinal microcracks"],"prefix":"10.1111","volume":"50","author":[{"given":"Gustavo","family":"De\u2010Deus","sequence":"first","affiliation":[{"name":"Department of Endodontics Fluminense Federal University  Niter\u00f3i Rio de Janeiro Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabio Nakao","family":"Arashiro","sequence":"additional","affiliation":[{"name":"Department of Endodontics School of 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